The combination of <em>Acalypha indica</em>–<em>Centella asiatica</em> extracts decreases the neuronal damage in hypoxia-induced hippocampal injury animal model

Abstract

Background: Approximately 80–85% of strokes are ischemic and lead to alterations in neuronal cell morphology and cell death. There is a lack of studies on the effect of the combination of Acalypha indica L. (AI) and Centella asiatica L. (CA) in terms of its neurotherapy property. This study was conducted to investigate the neurotherapeutic effect of the combination of AI–CA extracts in improving rat’s hippocampal neuron injury post-hypoxia.Methods: A total of 36 Sprague-Dawley rats were categorized into six groups and placed in a hypoxia chamber for 7 consecutive days. Then, they were moved to normoxia cages and treated for 7 consecutive days as follows: control group without treatment as a negative control; treatment groups were administered citicoline 50 mg/kgBW as a positive control; three different dose combinations of AI150–CA150, AI200–CA150, and AI250–CA150 mg/kgBW, respectively. Histological analyses were performed to assess the improvement in nerve cell damage in the hippocampus.Results: Treatment with citicoline significantly decreased the damage of nerve cells (30.8%); the combination of the AI–CA extracts of AI150–CA150, AI200–CA150, and AI250–CA150 also significantly decreased the damage of nerve cells (36%, 36.4%, and 30.4%, respectively) compared to the control rats (15.4%).Conclusion: The combination of AI–CA extracts decreased the neuronal damage in the hypoxia-induced hippocampal injury animal model. The improvement effect of the combination of AI–CA extracts was not significantly different to citicoline.

Hypoxia-Induced Neuroinflammation in Alzheimer’s Disease: Potential Neuroprotective Effects of Centella asiatica

“…In the same study, the authors showed that asiatic acid significantly improved cell survival and enhanced mitochondrial membrane potential in an oxygen–glucose deprivation-induced HT-22 hippocampal neuronal cell line ( Krishnamurthy et al, 2009 ). Similarly, a combination of CA extracts and Acalypha indica L (AI) decreased the neuronal cell damage in the hippocampus of hypoxia-induced injury rats model ( Farida et al, 2018 ). Furthermore, a later finding showed that CA extract exhibited its anti-oxidant effects by attenuating the reduction of body length in the hypoxia-exposed zebrafish group ( Ariani et al, 2019 ).…”

Acalypha Indica: Pemanfaatan dan Bioaktivitasnya

“…Tikus kelompokkan menjadi : kelompok kontrol tanpa treatmen sebagai kontrol negatif; kelompok perlakuan diberikan citicoline 50 mg/kgBB sebagai kontrol positif; tiga kombinasi dosis AI150-CA150, AI200 -CA150, dan AI250-CA150 mg/kgBB yang berbeda. Pemberian citicoline secara signifikan mengurangi kerusakan sel-sel saraf (30,8%); kombinasi ekstrak AI-CA dari AI150-CA150, AI200-CA150, dan AI250-CA150 juga secara signifikan mengurangi kerusakan sel-sel saraf sebanyak 36%; 36,4%; dan 30,4%, secara berurutan) dibandingkan dengan tikus kontrol (15,4%) (Farida et al 2018).…”