The combination of Raman microscopy and electron microscopy – Practical considerations of the influence of vacuum on Raman microscopy

The Raman Active Vibrations of Flavone and Quercetin: The Impact of Conformers and Hydrogen Bonding on Fingerprint Modes

“…Among analytical methods, Raman spectroscopy is unique due to its ability to measure detailed vibrational spectra on samples with essentially no sample preparation considering that it is a light scattering method . It can therefore be used to study molecules in their natural environment and can do so at high spatial resolution when coupled to optical, scanned probe, and electron microscopes. − Even single molecule spectra can be detected when the Raman scattering is enhanced by metal nanoparticle antennas. , DFT calculations can help identify the complex fingerprint vibrations of large molecules . Both Raman scattering and DFT have been applied to detect flavonoids and to help characterize the structure and stability of their microcrystalline powders. ,− Here, we use time dependent density functional theory (TDDFT) to identify Raman active vibrational modes of flavone and quercetin and to investigate the significant influence conformational variations and hydrogen bonding have on their spectra.…”

Pattern Formation in Catalytic H2 Oxidation on Rh: Zooming in by Correlative Microscopy

“… 24 , 25 In the last few decades, correlative analysis has made a vast impact in materials research, combining, e.g., transmission electron microscopy (TEM) with atom probe tomography (APT) 26 or Raman with SEM. 27 , 28 Using protective coatings, changes in the sample resulting from transfer (air exposure) between different devices can be avoided. 29 In catalysis, the correlative microscopy approach, often applied in different setups, 30 − 32 has reached its highest development stage when different microscopies, e.g., single-molecule fluorescence microscopy (SMF) and TEM are combined in one instrument.…”

“…The approach stems from biological research, where the first efforts of correlative light and electron microscopy (CLEM) on the same cell/tissue structures were made already in the 1970s. , In the meantime, CLEM has been extended to a broad category of methods combining any type of light and electron microscopy on the same sample . Actually, other imaging techniques such as atomic force microscopy (AFM), X-ray tomography, and scanning electron microscopy (SEM) are also successfully used in a correlative microscopy approach. , In the last few decades, correlative analysis has made a vast impact in materials research, combining, e.g., transmission electron microscopy (TEM) with atom probe tomography (APT) or Raman with SEM. , Using protective coatings, changes in the sample resulting from transfer (air exposure) between different devices can be avoided . In catalysis, the correlative microscopy approach, often applied in different setups, − has reached its highest development stage when different microscopies, e.g., single-molecule fluorescence microscopy (SMF) and TEM are combined in one instrument .…”