The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs

Tang, Yilong; Xu, Yan; Xiao, Zhifeng; Zhao, Yannan; Li, Jing; Han, Sufang; Chen, Lei; Dai, Bin; Chen, Bing; Wang, Hong

doi:10.1038/s41598-017-00087-x

Cited by 29 publications

(20 citation statements)

References 50 publications

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“…It has been argued that the best way to reproduce the cells' natural growth and differentiation in vitro is to mimic their microenvironments in vivo. 120,121 In this section, different 3D gelatin-based biomaterials will be discussed and compared. The techniques for creating these 3D gelatin biomaterials and their applications will be elaborated.…”

Section: Gelatin-based 3d Microenvironment For Cell Culturementioning

confidence: 99%

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Bello

Kim

et al. 2020

Tissue Engineering Part B: Reviews

399

259

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Health care and medicine were revolutionized in recent years by the development of biomaterials, such as stents, implants, personalized drug delivery systems, engineered grafts, cell sheets, and other transplantable materials. These materials not only support the growth of cells before transplantation but also serve as replacements for damaged tissues in vivo. Among the various biomaterials available, those made from natural biological sources such as extracellular proteins (collagen, fibronectin, laminin) have shown significant benefits, and thus are widely used. However, routine biomaterial-based research requires copious quantities of proteins and the use of pure and intact extracellular proteins could be highly cost ineffective. Gelatin is a molecular derivative of collagen obtained through the irreversible denaturation of collagen proteins. Gelatin shares a very close molecular structure and function with collagen and thus is often used in cell and tissue culture to replace collagen for biomaterial purposes. Recent technological advancements such as additive manufacturing, rapid prototyping, and three-dimensional printing, in general, have resulted in great strides toward the generation of functional gelatin-based materials for medical purposes. In this review, the structural and molecular similarities of gelatin to other extracellular matrix proteins are compared and analyzed. Current strategies for gelatin crosslinking and production are described and recent applications of gelatin-based biomaterials in cell culture and tissue regeneration are discussed. Finally, recent improvements in gelatin-based biomaterials for medical applications and future directions are elaborated.

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Section: Gelatin-based 3d Microenvironment For Cell Culturementioning

confidence: 99%

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Bello

Kim

et al. 2020

Tissue Engineering Part B: Reviews

399

259

View full text Add to dashboard Cite

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“…Osterix, Osteopontin, Collagen 1 A1) when compared to cells differentiated under 2D conditions. Also, cells derived from the 3D constructs differentiated in the rotational system showed significantly greater deposition capacity of the mineralized ECM when compared to cells derived from the 2D culture, as demonstrated by the Von Koss staining (Tang et al, 2017). Osteogenic differentiation of human MSCs derived from bone marrow also occurred more efficiently when grown in alginate beads.…”

Section: The Influence Of Three-dimensional Culture On Osteogenic Difmentioning

confidence: 84%

Impact of developmental origin, niche mechanics and oxygen availability on osteogenic differentiation capacity of mesenchymal stem/stromal cells

et al. 2019

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Mesenchymal Stem/Stromal Cells (MSCs) have been widely considered as a promising source of cells for tissue regeneration. Among other stem cells, they are characterized by a high osteogenic potential. Intensive studies in this field had shown that even if basic osteogenic differentiation is relatively simple, its clinical application requires more sophisticated approaches to prepare effective and safe cell therapy products. The aim of this review is to underline biological, physical and chemical factors which play a crucial role in osteogenic differentiation of MSCs. Existence of two distinct mechanisms of ossification (intramembranous and endochondral) indicate that choosing a proper source of MSCs may be critical for successful regeneration of a particular bone type. In this context, Dental Pulp Stem Cells representing a group of MSCs and originating from neural crest ( a structure responsible for development of cranial bones) are considered as the most promising for skull bone defect repair. Factors which facilitate osteogenic differentiation of MSCs include changes in forces exerted on cells during development. Thus, culturing of cells in hydrogels or on biocompatible three-dimensional scaffolds improves osteogenic differentiation of MSCs by both, the mechanotransductive and chemical impact on cells. Moreover, atmospheric oxygen concentration routinely used for cell cultures in vitro does not correspond to lower oxygen concentration present in stem cell niches. A decrease in oxygen concentration allows to create more physiological cell culture conditions, mimicking the ones in stem cell niches, which promote the MSCs stemness. Altogether, factors discussed in this review provide exciting opportunities to boost MSCs propagation and osteogenic differentiation which is crucial for successful clinical applications.

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“…In general, tissue engineered patches under dynamic conditions represented a more extensive tissue formation and ECM deposition. Wang et al also showed an enhanced cell proliferation under dynamic three-dimensional (3D) culture compared with conventional static two-dimensional (2D) and 3D cell culture conditions [39]. In addition, highthroughput microarray analysis showed that gene expressions of dynamic 3D system displayed significant differences in cell proliferation and differentiation compared with static-2D conditions [39].…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of poly-glycolic acid-based hybrid polymer starter matrices for in vitro tissue engineering

Generali

Kehl

Capulli

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Biodegradable scaffold matrixes form the basis of any in vitro tissue engineering approach by acting as a temporary matrix for cell proliferation and extracellular matrix deposition until the scaffold is replaced by neo-tissue. In this context several synthetic polymers have been investigated, however a concise systematic comparative analyses is missing. Therefore, the present study systematically compares three frequently used polymers for the in vitro engineering of extracellular matrix based on poly-glycolic acid (PGA) under static as well as dynamic conditions. Ultra-structural analysis was used to examine the polymers structure. For tissue engineering (TE) three human fibroblast cell lines were seeded on either PGA-poly-4-hydroxybutyrate (P4HB), PGA-poly-lactic acid (PLA) or PGA-poly-caprolactone (PCL) patches. These patches were analyzed after 21days of culture qualitative by histology and quantitative by determining the amount of DNA, glycosaminoglycan and hydroxyproline. We found that PGA-P4HB and PGA-PLA scaffolds enhance tissue formation significantly higher than PGA-PCL scaffolds (p<0.05). Polymer remnants were visualized by polarization microscopy. In addition, biomechanical properties of the tissue engineered patches were determined in comparison to native tissue. This study may allow future studies to specifically select certain polymer starter matrices aiming at specific tissue properties of the bioengineered constructs in vitro.

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The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs

Cited by 29 publications

References 50 publications

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Engineering and Functionalization of Gelatin Biomaterials: From Cell Culture to Medical Applications

Impact of developmental origin, niche mechanics and oxygen availability on osteogenic differentiation capacity of mesenchymal stem/stromal cells

Comparative analysis of poly-glycolic acid-based hybrid polymer starter matrices for in vitro tissue engineering

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