The combination of three molecular markers can be a valuable predictive tool for the prognosis of hepatocellular carcinoma patients

Abstract

Based on molecular profiling, several prognostic markers for HCC are also used in clinic, but only a few genes have been identified as useful. We collected 72 post-operative liver cancer tissue samples. Genes expression were tested by RT-PCR. Multilayer perceptron and discriminant analysis were built, and their ability to predict the prognosis of HCC patients were tested. Receiver operating characteristic (ROC) analysis was performed and multivariate analysis with Cox’s Proportional Hazard Model was used for confirming the markers’predictive efficiency for HCC patients’survival. A simple risk scoring system devised for further predicting the prognosis of liver tumor patients. Multilayer perceptron and discriminant analysis showed a very strong predictive value in evaluating liver cancer patients’prognosis. Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery. Finally, the risk scoring system revealed that patients whose total score >1 and >3 are more likely to relapse and die than patients whose total score ≤1 and ≤3. The three genes model proposed proved to be highly predictive of the HCC patients’ prognosis. Implementation of risk scoring system in clinical practice can help in evaluating survival of HCC patients after operation.

Paradoxical roles of dual oxidases in cancer biology

“…3). Contrasting these findings, Knaus and colleagues originally reported that expression of both DUOX1 and DUOX2 is typically suppressed in lung cancer cell lines compared to normal epithelial cells [52], and subsequent studies confirmed that DUOX1 is frequently suppressed in both lung and hepatic cancers[13, 53, 111]. Analysis of TCGA data confirms that DUOX1 is commonly suppressed in various cancers, primarily those of lung and skin, compared to adjacent healthy tissues (Fig.…”

“…4). Indeed, ectopic expression of DUOX1 in liver cancers was found to correlate strongly with overall patient survival and thus was proposed as a marker for patient prognosis [53], suggests that DUOX1 silencing may be of similar importance in progression of others cancers including NSCLCs.…”

“…tumor progression or metastasis, thus explaining its association with poor prognosis in e.g. HCC [53]. …”

“…As will be discussed later in this review, alternative roles for DUOXA1 independent of DUOX1 have been described that may be relevant for cancer biology. Mammalian DUOX1 and 2 were first cloned from thyrocytes and were originally characterized as thyroid oxidase for its role in thyroid hormone synthesis[122, 123], but are now known to be more widely expressed in various epithelial tissues in the lung, pancreas, placenta, gastrointestinal tract, testis, liver, prostate, salivary glands, skin, and brain[53, 111, 124–126]. …”

A sensitive three monoclonal antibodies based automatic latex particle-enhanced turbidimetric immunoassay for Golgi protein 73 detection

“…Hepatocellular carcinoma (HCC) is currently ranked among the most common primary malignant cancers worldwide and is the third and fifth leading cause of death from cancer globally in men and women, respectively123. Due to lack of effective strategies for early diagnosis and pre-clinical screening for HCC in high-risk populations, the majority of patients can be treated only with loco regional therapies, resulting in limited survival benefits and tumor recurrence in 50–80% of patients at 5 years after treatment45.…”