The aim of this research was to study the effect of foliar spray with BA and B-9 on essential oil production and chemical composition of marjoram plant. The plants were sprayed with BA at the rates of 50, 100 and 150 ppm, while B-9 at the rates of 500, 1000 and 2000 ppm in addition to control treatment which sprayed with distilled water. The obtained results can be summarized as follows: BA at the rates of 50, 100 and 150 ppm or B-9 at the rates of 500, 1000 and 2000 ppm increased all recorded parameters as compared to control treatment with significant differences in most cases during the two seasons. In the first season, the highest value of essential oil percentage was produced with B-9 at 1000 ppm, while the highest values of essential oil yield were obtained with BA at 50, 150 ppm and with B-9 at 1000 ppm treatments. In the second season the highest values of essential oil percentage and essential oil yield were produced with BA at 50 and 100 ppm, respectively. The major essential oil components were linalyl acetate and terpinen-4-ol. The highest percentage of linalyl acetate was detected in the oil of the plants sprayed with B-9 at 1000 ppm in the two seasons, while in the first season, the highest percentage of terpinen-4-ol was found in oil of the plants sprayed with B-9 at 500 ppm as well as the highest percentage of terpinen-4-ol in the second season was produced from control treatment followed by B-9 at 500 ppm. In the first season, the highest values of chlorophyll a, b and carotenoids were produced with BA at 50 ppm, B-9 at 2000 and 500 ppm, respectively. While in the second season, the highest values of chlorophyll a, b and carotenoids were obtained from B-9 at 2000 ppm, BA at 150 ppm and BA at 150 ppm, respectively. The highest values of total carbohydrates percentage were resulted in B-9 at 2000 ppm in the two seasons. In the first season, the highest total seasonal yield of essential oil was obtained from B-9 at 1000 ppm, while in the second season, the highest value was produced from BA at 100 ppm. Generally in combined statistical analysis B-9 at 1000 ppm was the most effective treatment in increasing total seasonal yield of essential oil. The lowest values of all recorded parameters were produced from the control plants in the two seasons. Different cuts as general mean had a significant effect on all recorded parameters in the two seasons. The highest values of essential oil percentage, chlorophyll a, b and carotenoids were produced from the third cut in the two seasons. Also, the highest values of total carbohydrates percentage were produced from the second cut and third cut in the first and second seasons, respectively. The highest values of essential oil yield were obtained from the third cut and fourth cut in the first and second seasons, respectively. The interaction between the growth regulators (BA or B-9) and the cuts increased all recorded parameters as compared with the control in any cut during the two seasons with significant differences in most cases. Majorana hortensis plant bel...