The combined treatments of brassinolide and zeaxanthin better alleviate oxidative damage and improve hypocotyl length, biomass, and the quality of radish sprouts stored at low temperature

Li, Minghui; Zhang, Yanfen; Xu, Xihang; Chen, Ying; Chu, Jianzhou; Yao, Xiaogang

doi:10.1016/j.fochx.2022.100394

Cited by 5 publications

(4 citation statements)

References 68 publications

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“…It was also reported that radish seed treatment with 24-epibrassinolide (0.001 μmol L −1 ) could improve the antioxidant activities, as well as restrain the free radical capacity in seed sprouts under copper ion (Cu ++ ) stress [ 54 ]. Li et al [ 55 ] reported that brassinolide treatment improved the POD activity, SOD activity, and ABTS levels in radish sprouts preserved at a low temperature. Cao et al [ 56 ] revealed that BR elevated SOD and POD activities by enhancing the expression of the DET2 gene.…”

Section: Discussionmentioning

confidence: 99%

“…The melon seedlings were grown in a greenhouse at the required temperature range of 23 °C–28 °C and humidity of 50–80%. Twenty-day-old (about three leaf stage) seedlings were artificially infected with fungal spores of downy mildew disease and divided into two respective groups: the first group was simply sprayed with distilled water (ddH 2 O) to retain the moisture and marked as control (CK, without BR treatment); the second group was exogenously sprayed with different treatments of slightly modified concentrations of BR solution (0.5, 1.0, and 2.0 mg·L −1 ) [ 55 ], to check the effectiveness of BR treatment for maintaining the growth and physiological indices of the melon plant against downy mildew disease stress. Then, the developed seedlings were inspected regularly based on the development of DM symptoms, and leaves were collected at specific time periods of 6 h, 12 h, 24 h, and 48 h from the CK- and BR-treated groups.…”

Section: Methodsmentioning

confidence: 99%

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Deciphering the Enhancing Impact of Exogenous Brassinolide on Physiological Indices of Melon Plants under Downy Mildew-Induced Stress

Liu,

Xu,

Amanullah

et al. 2024

Plants

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Melon (Cucumis melo L.) is a valuable horticultural crop of the Cucurbitaceae family. Downy mildew (DM), caused by Pseudoperonospora cubensis, is a significant inhibitor of the production and quality of melon. Brassinolide (BR) is a new type of phytohormone widely used in cultivation for its broad spectrum of resistance- and defense-mechanism-improving activity. In this study, we applied various exogenous treatments (0.5, 1.0, and 2.0 mg·L−1) of BR at four distinct time periods (6 h, 12 h, 24 h, and 48 h) and explored the impact of BR on physiological indices and the genetic regulation of melon seedling leaves infected by downy-mildew-induced stress. It was mainly observed that a 2.0 mg·L−1 BR concentration effectively promoted the enhanced photosynthetic activity of seedling leaves, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis similarly exhibited an upregulated expression of the predicted regulatory genes of photosystem II (PSII) CmHCF136 (MELO3C023596.2) and CmPsbY (MELO3C010708.2), thus indicating the stability of the PSII reaction center. Furthermore, 2.0 mg·L−1 BR resulted in more photosynthetic pigments (nearly three times more than the chlorophyll contents (264.52%)) as compared to the control and other treatment groups and similarly upregulated the expression trend of the predicted key enzyme genes CmLHCP (MELO3C004214.2) and CmCHLP (MELO3C017176.2) involved in chlorophyll biosynthesis. Meanwhile, the maximum contents of soluble sugars and starch (186.95% and 164.28%) were also maintained, which were similarly triggered by the upregulated expression of the predicted genes CmGlgC (MELO3C006552.2), CmSPS (MELO3C020357.2), and CmPEPC (MELO3C018724.2), thereby maintaining osmotic adjustment and efficiency in eliminating reactive oxygen species. Overall, the exogenous 2.0 mg·L−1 BR exhibited maintained antioxidant activities, plastid membranal stability, and malondialdehyde (MDA) content. The chlorophyll fluorescence parameter values of F0 (42.23%) and Fv/Fm (36.67%) were also noticed to be higher; however, nearly three times higher levels of NPQ (375.86%) and Y (NPQ) (287.10%) were observed at 48 h of treatment as compared to all other group treatments. Increased Rubisco activity was also observed (62.89%), which suggested a significant role for elevated carbon fixation and assimilation and the upregulated expression of regulatory genes linked with Rubisco activity and the PSII reaction process. In short, we deduced that the 2.0 mg·L−1 BR application has an enhancing effect on the genetic modulation of physiological indices of melon plants against downy mildew disease stress.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Deciphering the Enhancing Impact of Exogenous Brassinolide on Physiological Indices of Melon Plants under Downy Mildew-Induced Stress

Liu,

Xu,

Amanullah

et al. 2024

Plants

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“…Various studies have indicated the efficacy of BR treatment in enhancing phenolic biosynthesis in a multitude of harvested horticultural crops, including carambola fruit, 13 tomato fruit 14 and pomegranate fruit 15 . Furthermore, the effectiveness of BR can be further enhanced through combination with other treatments, such as zeaxanthin 16 and UV‐B radiation 17 . In addition, BR regulates sugar and energy metabolism in postharvest horticultural crops during storage.…”

Section: Introductionmentioning

confidence: 99%

Exogenous brassinolide treatment regulates phenolic accumulation in mung bean sprouts through the modulation of sugar and energy metabolism

Wang,

Chen,

Guo

et al. 2023

J Sci Food Agric

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BACKGROUNDThe effects of exogenous brassinolide (BR) treatment (3.0 μM) on phenolic biosynthesis in mung bean sprouts were investigated. This investigation included the analysis of sugar content, substrates within the phenylpropane pathway, energy substances, enzymatic activity within the phenylpropane pathway, sugar metabolism and energy metabolism.RESULTSResults showed that BR treatment significantly increased the levels of total phenolics, p‐hydroxybenzoic acid p‐coumaric acid, gallic acid, fumalic acid, and caffeic acid. This enhancement was accomplished through the elevation of L‐phenylalanine levels and the activation of enzymes associated with the phenylpropane pathway in mung bean sprouts, including phenylalanine ammonia‐lyase, cinnamate 4‐hydroxylase and 4‐coumarate CoA ligase. Furthermore, BR treatment induced alterations in sugar metabolism in mung bean sprouts as evidenced by the increased levels of glucose, fructose, sucrose and phosphoenolpyruvate. Moreover, increased activity was observed for enzymes linked to sucrose metabolism and glycolysis in the BR‐treated group. Concurrently, BR treatment bolstered the levels of adenosine triphosphate and energy charge in mung bean sprouts, which was attributed to the activation of H+‐adenosine triphosphatase, Ca2+‐adenosine triphosphatase and succinic dehydrogenase.CONCLUSIONThese results suggest that BR treatment can accelerate the accumulation of phenolic compounds in mung bean sprouts. This effect is achieved not only through the activation of the phenylpropane pathway, but also through the modulation of sugar and energy metabolism. The modulation provides ample energy and a substrate for the biosynthesis of phenolics.This article is protected by copyright. All rights reserved.

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“…Additionally, EBR can increase the inhibition of photosynthesis, the activities of antioxidase and Rubisco activase (RCA), and the gene expression in wheat induced by drought and heat stress [ 10 ]. BR has been shown to alleviate oxidative damage and improve hypocotyl length, biomass, and sprout quality in radishes stored at low temperature by increasing the activities of POD and SOD [ 11 ]. EBR improved drought tolerance of tobacco by increasing the activities of SOD, POD, and CAT enzymes and the expressions of their related genes, along with a higher accumulation of osmoregulatory substances [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroids Regulate the Water Deficit and Latex Yield of Rubber Trees

Guo

Yang

et al. 2023

IJMS

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Brassinolide (BR) is an important plant hormone that regulates the growth and development of plants and the formation of yield. The yield and quality of latex from Hevea brasiliensis are regulated by phytohormones. The understanding of gene network regulation mechanism of latex formation in rubber trees is still very limited. In this research, the rubber tree variety CATAS73397 was selected to analyze the relationship between BR, water deficit resistance, and latex yield. The results showed that BR improves the vitality of rubber trees under water deficit by increasing the rate of photosynthesis, reducing the seepage of osmotic regulatory substances, increasing the synthesis of energy substances, and improving the antioxidant system. Furthermore, BR increased the yield and quality of latex by reducing the plugging index and elevating the lutoid bursting index without decreasing mercaptan, sucrose, and inorganic phosphorus. This was confirmed by an increased expression of genes related to latex flow. RNA-seq analysis further indicated that DEG encoded proteins were enriched in the MAPK signaling pathway, plant hormone signal transduction and sucrose metabolism. Phytohormone content displayed significant differences, in that trans-Zeatin, ethylene, salicylic acid, kinetin, and cytokinin were induced by BR, whereas auxin, abscisic acid, and gibberellin were not. In summary, the current research lays a foundation for comprehending the molecular mechanism of latex formation in rubber trees and explores the potential candidate genes involved in natural rubber biosynthesis to provide useful information for further research in relevant areas.

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The combined treatments of brassinolide and zeaxanthin better alleviate oxidative damage and improve hypocotyl length, biomass, and the quality of radish sprouts stored at low temperature

Cited by 5 publications

References 68 publications

Deciphering the Enhancing Impact of Exogenous Brassinolide on Physiological Indices of Melon Plants under Downy Mildew-Induced Stress

Deciphering the Enhancing Impact of Exogenous Brassinolide on Physiological Indices of Melon Plants under Downy Mildew-Induced Stress

Exogenous brassinolide treatment regulates phenolic accumulation in mung bean sprouts through the modulation of sugar and energy metabolism

Brassinosteroids Regulate the Water Deficit and Latex Yield of Rubber Trees

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