The comparative atopic activity of alternaria spores and mycelium

Pratt, Henry N.

doi:10.1016/s0021-8707(42)90161-8

Cited by 18 publications

(3 citation statements)

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“…Thus the antigens detected in the medium may originate main ly from the mycelium. Furthermore, the mycelia of moulds have been shown to be a mi nor source of clinically important antigens as compared to the conidia [Pratt and Crossman, 1942]. The medium was there fore chosen to compromise between reasona ble growth rate and an encouragement of co nidia formation, and the extraction was based upon the mats and did not include the medi um.…”

Section: Discussionmentioning

confidence: 99%

Cladosporium herbarum Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

Løwenstein¹,

Aukrust²,

Gravesen³

1977

Int Arch Allergy Immunol

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Freeze-dried extract of Cladosporium herbarum Link ex Fr. was obtained by growing, harvesting, extracting, centrifuging, dialysing and freeze-drying. Quantitative immunoelectrophoresis using rabbit antibodies revealed the extraction procedure to be reproducible and the extract to be composed of 57 antigens, none of which originated from the substrate used in the growth. The molecular weight distribution and the approximate molecular weight of some antigens of C. herbarum were obtained using gel filtration. The pI distribution and the approximate pIs of a few distinct antigens of C. herbarum were obtained by isoelectric focusing. Preliminary identification of 4 allergens from C. herbarum was performed by means of CRIE (crossed radioimmunoelectrophoresis).

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Section: Discussionmentioning

confidence: 99%

Cladosporium herbarum Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

Løwenstein¹,

Aukrust²,

Gravesen³

1977

Int Arch Allergy Immunol

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“…In stud ies on Alternaria alternate!, Schumacher and Jeffery [20] observed biochemical differ ences between culture filtrates from 7 differ ent isolates, and between 4 batches of cul ture filtrates from the same isolate. Pratt [18] reported that the allergenicity of the spores of this mold was 10 times higher than that of the mycelium.…”

Section: Introductionmentioning

confidence: 99%

Crossed Radioimmunoelectrophoretic Studies of Distinct Allergens in Two Extracts of Cladosporium herbarum

Aukrust¹

1979

Int Arch Allergy Immunol

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Cladosporium herbarum was supplied from two sources and extracted identically. Antisera against the extracts were produced in rabbits and two reference patterns were established using crossed immunoelectrophoresis (CIE). Both patterns showed more than 60 precipitates but less than 50% of the detectable antigens appeared to be identical in the two extracts. The allergens were identified by means of crossed radioimmunoelectrophoresis (CRIE) using sera from 35 individuals with proven or suspected allergy to C. herbarum. Four important and 10–20 less important allergens were demonstrated. Among the allergens present, there were none reacting with all patient sera. Only 1 out of 3 rabbits immunized with a suspension of broken cells of C. herbarum showed precipitating antibodies to the statistically most important allergen, while 9 rabbits immunized with aqueous extracts of the mold did not. The composition of the two extracts with respect to allergens differed. Allergens present in one extract were not always detectable in the other. The experiments also showed how CIE/CRIE with various combinations of antigens and antisera may be combined with CRIE inhibition, radioallergosorbent test (RAST) and RAST inhibition for comparing complex allergen extracts at the molecular level.

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“…

Inbalation of air-borne spores elicits tbe symptoms of mould allergy. A few investigators (Feinberg 1946, Pratt 1941, Pratt and Crossmann 1941 bave demonstrated tbat the allergen is primarily localized to tbe spores. Therefore, extracts of fungi for test or treatment purposes should always be derived from spores.

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confidence: 99%

CULTURE OF FUNGI ON SOLID MEDIUM. PRODUCTION OF DRY PREPARATIONS FOR TESTING¹

Samsøe-Jensen¹

1955

Allergy

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The comparative atopic activity of alternaria spores and mycelium

Cited by 18 publications

References 0 publications

<i>Cladosporium herbarum</i> Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

<i>Cladosporium herbarum</i> Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

Crossed Radioimmunoelectrophoretic Studies of Distinct Allergens in Two Extracts of <i>Cladosporium herbaru</i><i>m</i>

CULTURE OF FUNGI ON SOLID MEDIUM. PRODUCTION OF DRY PREPARATIONS FOR TESTING¹

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The comparative atopic activity of alternaria spores and mycelium

Cited by 18 publications

References 0 publications

<i>Cladosporium herbarum</i> Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

<i>Cladosporium herbarum</i> Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

Crossed Radioimmunoelectrophoretic Studies of Distinct Allergens in Two Extracts of <i>Cladosporium herbaru</i><i>m</i>

CULTURE OF FUNGI ON SOLID MEDIUM. PRODUCTION OF DRY PREPARATIONS FOR TESTING1

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CULTURE OF FUNGI ON SOLID MEDIUM. PRODUCTION OF DRY PREPARATIONS FOR TESTING¹