The Comparative PDT Experiment of the Inactivation of HL60 on Modified TiO₂ Nanoparticles

Abstract

Four samples of modified titanium dioxide (TiO2), Fe/TiO2(2 wt%), Fe/TiO2(5 wt%), and 5-ALA/TiO2, were experimented in photodynamic therapy (PDT) on leukemia cells HL60, performing promising photocatalytic inactivation effect. Fe/TiO2and 5-ALA/TiO2were synthesized in methods of precipitation and ultrasonic methods, respectively. X-ray diffraction spectra and UV-Vis spectra were studied for the samples’ crystalline phase and redshift of absorption peak. Further, FTIR spectra and Raman spectra were obtained to examine the combination of 5-aminolevulinic (5-ALA) and TiO2nanoparticles. The toxicity of these four kinds of nanoparticles was studied through darkroom experiments. And based on the concentration which caused the same toxic effect (90%) on HL60, PDT experiments of TiO2, Fe/TiO2(2%), Fe/TiO2(5%), and ALA/TiO2were done, resulting in the fact that the photokilling efficiency was 69.7%, 71.6%, 72%, and 80.6%, respectively. Scanning electron microscope (SEM) images of the samples were also taken to study the morphology of HL60 cells before and after PDT, resulting in the fact the activation of the modified TiO2from PDT was the main cause of cell apoptosis.

Synergistic effect of TEMPO-coated TiO2 nanorods for PDT applications in MCF-7 cell line model

“…These results showed that with an increase in the UV exposure time, the cell viability decreased. The results of this research are in good agreement with the previous published data ( Lu et al, 2015 ). Toxicity occurs because there is an excess of low density lipoprotein (LPL) receptors in liver cancer tissue, which attracts more antioxidant agents occupied by TEMPO-treated TiO 2 NRs.…”

Semiconductor Quantum Dots (CdX, X = S, Te, Se) Modify Titanium Dioxide Nanoparticles for Photodynamic Inactivation of Leukemia HL60 Cancer Cells

“…As reported in our previous work, coupling TiO 2 with low band gap energy metal or nonmetal to form a heterostructure is a feasible strategy to extend the visible light response range of TiO 2 -based nanocomposites and thus improve the photocatalytic performance [13][14][15][16]. CdS, CdSe, and CdTe belong to the II-VI group cadmium-based quantum dot material [17,18].…”