The comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse

McCarthy, Helen Elizabeth; Bara, Jennifer J.; Brakspear, Karen Sarah; Singhrao, Simarjit Kaur; Archer, Charles W.

doi:10.1016/j.tvjl.2011.08.036

Cited by 152 publications

(166 citation statements)

References 32 publications

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“…BM-MSCs also express hypertrophic characteristics during chondrogenic differentiation [16] and, regardless, the joints sampled in these experiments were clinically normal and did not have sufficient cartilage loss to provide direct communication with the subchondral marrow compartment. In contrast, progenitors from the articular cartilage surface express a non-hypertrophic chondrogenic phenotype [37], consistent with the outcomes of our experiments. In healthy joints, it is highly plausible that the low numbers of SF-CPs are shed directly from the cartilage surfaces.…”

Section: Discussionsupporting

confidence: 90%

“…Progenitor cell populations have been identified in several intra-and peri-articular tissues; synovial membrane [34], subchondral bone marrow space [35], intra-articular adipose tissue [36], and articular cartilage surface [37]. Adipose-derived MSCs [14] and synovial membrane-derived progenitors [15] express a hypertrophic phenotype under chondrogenic conditions.…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Chen¹,

Bianchessi²,

Pondenis³

et al. 2016

Stem Cell Biol Res

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Background: Progenitor cells exist in most tissues and body fluids. Synovial fluid chondroprogenitor cells have been described in several species; however, the specific phenotypic characteristics of these cells have not been defined. This study addressed the impacts of joint location and donor age variation on synovial fluid chondroprogenitor numbers, and determined whether synovial fluid chondroprogenitors express hypertrophic characteristics or a non-endochondral phenotype in chondrogenic culture. Methods: Synovial fluid was aspirated from the joints of healthy adult horses, and cells in the aspirates were expanded through two monolayer passages. The number of colony-forming units (CFU) in each aspirate was assessed after 14 days. Population doublings (PD) and PD times were calculated during the subsequent two passages. Passage 3 cells were used to determine osteogenic and adipogenic capacities, or were transferred to pellet cultures in chondrogenic medium containing TGF-β1 or BMP-2. Bone marrowderived MSCs and primary articular chondrocytes were cultured under similar conditions to provide reference values for chondrogenesis. Chondrogenesis was assessed by measuring collagen type II protein and sulfated glycosaminoglycan secretion, expression of chondrogenic mRNAs, and induction of ALP activity. Results: CFU counts varied considerably, but the majority of aspirates contained <50 CFU/ml. PDs were consistently between 2.0 and 3.0 during both passages. PD times varied from 0.2-0.4 days. Proliferation was not significantly influenced by anatomical location or donor age. Equine synovial fluid progenitors expressed osteogenic, adipogenic and chondrogenic phenotypes under appropriate conditions. Under chondrogenic conditions, synovial fluid cells increased collagen and aggrecan mRNA expression to levels comparable to bone marrow MSCs, although collagen type II protein secretion was less than half that of articular chondrocytes. In contrast to bone marrow MSCs, synovial fluid chondroprogenitors did not express collagen type X or increase ALP activity in response to TGF-β1 or BMP-2. Consistent with these observations, Runx2 and Mef2C expression in synovial fluid chondroprogenitors was 20-40 fold lower than in bone marrow MSCs. Conclusions: Synovial fluid chondroprogenitors are capable of robust non-hypertrophic chondrogenesis, whether stimulated by TGF-β1 or by BMP-2. These results indicate that synovial fluid cells are phenotypically suitable for articular cartilage repair/regeneration, although strategies to accelerate cell proliferation and synthesize a functional cartilage matrix in vivo will be required for clinically feasible cellbased therapies.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Chen¹,

Bianchessi²,

Pondenis³

et al. 2016

Stem Cell Biol Res

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“…These cells possess characteristics similar to stem cells isolated from other adult tissues involving proliferation and differentiation potential under appropriate in vitro conditions [120][121][122][123]. They were subjected to the process of isolation, expansion, and identification in order to confirm their stem cells phenotype previously established on MSC from other adult tissues [121][122][123][124]. To date, studies investigated the presence of these cells in normal and OA cartilage.…”

Section: Chondroprogenitors Potential In Cartilage Repairmentioning

confidence: 99%

“…[105]. ACPCs may therefore be considered superior to MSCs from other tissues in cartilage repair [124,125,128,129].…”

Section: Chondroprogenitors Potential In Cartilage Repairmentioning

confidence: 99%

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

Toumi¹,

Lespessailles²,

Mazor³

2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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Although many surgical and pharmaceutical interventions are currently available for treating osteoarthritis (OA), restoration of normal cartilage function remains inefficient.In fact, because of the absence of vasculature within the articular cartilage (AC), the selfpotential for regeneration is very poor. Recently, researchers and clinicians have been focusing on alternative methods for cartilage preservation and repair. It has been shown that AC contains a population of stem cells or progenitor cells, similar to those found in many other adult tissues that are thought to be involved in the maintenance of tissue homeostasis. In the present chapter, we review the current status of stem cells potential in the treatment of early OA and discuss the possible origin of these cells and the role they might have in cartilage repair. We also review the recent progress in the field of chondroprogenitors in cartilage.

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“…The paraffin section was probed to detect type-II collagen (Developmental Studies Hybridoma Bank, Iowa City, Iowa) and type-X collagen. Control sections not exposed to the primary antibody were probed with normal serum specific to the corresponding primary antibody 8,12 . The percentage of the area of the repair tissue that exhibited positive staining (as determined by comparison with the control) was subjectively assessed.…”

Section: Postoperative Evaluationmentioning

confidence: 99%

Evaluation of Articular Cartilage Progenitor Cells for the Repair of Articular Defects in an Equine Model

Frisbie

McCarthy

Archer

et al. 2015

The Journal of Bone and Joint Surgery

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The comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse

Cited by 152 publications

References 32 publications

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

Evaluation of Articular Cartilage Progenitor Cells for the Repair of Articular Defects in an Equine Model

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