The Comparison of Exosome RNA Concentration and its LMP-1 and p53 content Among Patients with Nasopharyngeal Cancer before and after Chemoradiation

Abstract

ObjectiveNasopharyngeal cancer (NPC) remains the highest head and neck malignancy in Asia, especially in Southeast Asia. The exosome is a nanocarrier that has a role in inhibit or promote the progression of cancer as well as the marker to knows the response of therapy depending on its carrier inside. NPC-exosome carriers including LMP-1 and p53. LMP-1 is a main oncogene of Epstein Barr Virus (EBV) the predominant cause of NPC. Wild type p53 known as the main inhibitor of cancer growth. The study aims to compare the concentration of exosomes and the characteristic of LMP-1 and p53 inside exosomes among patients with NPCResultEight patients fulfilled the study criteria. The mean age was 37,5 years old (SD = 9,9), most of them were stage III. The mean exosome RNA concentration before and after chemoradiation was 14,39 and 3,61 ng/ml with a p-value of 0,04. LMP-1 expression was documented inside the exosome. Expression of p53 in exon 4-5 and 5-6 are express well, except on exon 7-8. Furthermore, there was a significant increase of p53 expression relative to the housekeeping gene in exon 4-5 and 5-6 after chemoradiation with a p-value of 0,001 and 0,002 consecutively.