The Compartmentalization of Antigen-Reactive Lymphocytes in Desensitized Guinea Pigs

Abstract

A single injection of ϵ,DNP-Lys7–10 can render previously sensitized guinea pigs specifically unreactive to subsequent intradermal challenge with that antigen. Antigen-reactive lymphocytes, as assayed by macrophage-migration in-inhibition or thymidine incorporation, were depleted from the peritoneal exudates of those animals. In contrast, it was intriguing to find that lymph node lymphocytes from such animals responded normally in the antigen-induced thymidine incorporation assay. These studies demonstrate a compartmentalization of antigen-reactive lymphocytes in desensitized animals which may account for the short-lived nature of this phenomenon.

Modulation of cellular-immune responses in vivo and in vitro by histamine receptor-bearing lymphocytes.

“…Lymph node lymphocytes from OCB-BGG immune animals were cultured in vitro for [3H] thymidine incorporation (10). 107 cells/2.0 ml were cultured in TC-199 containing 15% normal guinea pig serum and 50 ,tg/ml OCB-BGG with or without drugs for 4 days at 370C in a 5% C02-95% air atmosphere.…”

Immune Mechanisms Affecting Bovine Leucocytes during Suppression of Tuberculin Skin Sensitivity in Cattle

“…More granulomata might have been ex pected in lymphoid tissue in view of the large numbers of living BCG organisms in jected. If sensitized lymphocytes were sequestered in the lymphoid tissue, as sug gested by Schlossman et al [1971], it would be expected that they would reappear in the blood soon after the desensitizing injections ceased. However, this did not happen in the completely desensitized cattle within the time limit of our experiment.…”

“…Schlossman et al [1971], re ported that this treatment in guinea pigs was followed by unresponsiveness to antigen in peritoneal exudate cells but not lymph-node cells. This was interpreted to mean that an tigen-reactive peripheral lymphocytes in in flammatory exudates or skin were depleted but those in the lymph nodes were not af fected.…”

<i>In vitro </i>DNA Synthesis in Lymphocytes from DNCB Contact-Sensitive Guinea Pigs

“…This dissociation (14,21) can be explained on the ground of differing mechanisms of tolerance and desensitization (16). Tolerance in contact sensitivity is mediated by suppressor cells acting on the afferent branch of the immune response thus preventing the development of both effector and memory cells.…”