The Complete Amino Acid Sequence of Dog &amp;beta;&lt;sub&gt;2&lt;/sub&gt;-Microglobulin

Nakajima, Yuichi; Hoshi, Fumio; Higuchi, Seiichi; Kawamura, Seiichi

doi:10.1292/jvms.61.517

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…Finally, the four different bovine urine samples were analyzed for the presence of BSA. No BSA was found, suggesting that the concentration of BSA is lower than 170 nmol/l, which may be in accordance with a study of Nakajima et al [32], who showed a concentration of 307 nmol/l in bovine urine. However, in this off-line procedure the sample volume was 50 ml and the relative standard deviation was ca.…”

Section: Application To Bovine Urine Samplessupporting

confidence: 91%

Selective quantitative bioanalysis of proteins in biological fluids by on-line immunoaffinity chromatography–protein digestion–liquid chromatography–mass spectrometry

Hoos

Sudergat²,

Hoelck³

et al. 2006

Journal of Chromatography B

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Section: Application To Bovine Urine Samplessupporting

confidence: 91%

Selective quantitative bioanalysis of proteins in biological fluids by on-line immunoaffinity chromatography–protein digestion–liquid chromatography–mass spectrometry

Hoos

Sudergat²,

Hoelck³

et al. 2006

Journal of Chromatography B

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“…The protocol used for isolation of canine β 2 -m from the urine was reported previously [11]. For preparation of antiserum, a rabbit was immunized by the injection of 1 mg of β 2 -m with complete Freund's adjuvant (ORGA-NON TECHNIKA Co., Belgium) intracutaneously into its back.…”

mentioning

confidence: 99%

Determination of Canine .BETA.2-Microgloblin in Plasma and Urine by Enzyme-linked Immunosorbent Assay.

Nakajima

Hoshi

Higuchi

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. An enzyme-linked immunosorbent assay was developed for the determination of canine β 2 -microglobulin (β 2 -m) in plasma and urine. The detectable sensitivity for pure canine β 2 -m was 0.05 µg/l and the analytical range was 0.1 to 50 µg/l. The mean analytical recovery when pure canine β 2 -m was added to normal plasma was 101.9%. The mean analytical recovery in the urine was 102.1%. The intra-day variation coefficient was 3.1% in plasma, 4.3% in serum and 1.9% in urine. No difference was found between the concentration of β 2 -m in plasma and serum (n=17). The concentration of β 2 -m in the plasma of normal dogs was 1.82 ± 0.57 mg/l (n=31). The mean excretion in 24 hr urine collected from normal dogs was 17.6 ± 9.2 µg/l, 0.22 ± 0.12 µg/kg of body weight or 14.2 ± 9.4 µg/g of urine creatinine. The β 2 -m creatinine index of random urine samples was 23.5 ± 16.6 µg/g (n=26). There was a close correlation between the β 2 -m creatinine index of 24 hr urine samples and that of random urine samples (r= 0.872). KEY WORDS: canine, ELIS, β 2 -microglobulin.J. Vet. Med. Sci. 63(3): 343-345, 2001 β 2 -microglobulin (β 2 -m) is a low molecular weight protein that is present on the surface of all nucleated cells [12]. This protein is the light chain of the class I major histocompatibility antigens [13]. Determination of serum β 2 -m and the urine β 2 -m concentration is used to evaluate renal function in man [3,9]. The determination of β 2 -m has been carried out by radioimmunoassay [4], enzyme-linked Immunosorbent assay (ELISA) [2] and immunoturbidimetry [1], but there are no reports on the measurement of β 2 -m concentrations in dog body fluids. In this study, we developed an ELISA test for determination of β 2 -m in the plasma, serum and urine of dogs.Purified canine β 2 -m and antiserum were prepared in our laboratory. The protocol used for isolation of canine β 2 -m from the urine was reported previously [11]. For preparation of antiserum, a rabbit was immunized by the injection of 1 mg of β 2 -m with complete Freund's adjuvant (ORGA-NON TECHNIKA Co., Belgium) intracutaneously into its back. The rabbit was injected every three weeks for a total of 3 times. The IgG fraction of the antiserum was precipitated with ammonium sulfate and then purified by means of an Econo-Pac Protein A Cartridge (Bio-Rad Laboratories, U.S.A.) according to Tarditi et al. [14]. The purified IgG was dialyzed against ammonium acetate buffer (pH 8.6, 4°C), lyophilized and stored at 4°C. The IgG fraction was conjugated with biotin (biotinyl-N-hydroxysuccinimide ester, EY Laboratories, U.S.A.) by a method similar to that of Guesdon et al. [5].Blood samples were obtained from 32 normal dogs. Plasma was isolated from 3 ml of whole blood, with heparin as anticoagulant. In addition, simultaneous serum samples were taken from 17 dogs. Twenty-four hour urine samples and random urine samples were collected from 26 normal dogs, and each urine sample was stored in several PE-tubes. Five µl of Tween 20 (Kantokagaku Co., Ltd., Japan) was added to ...

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“…However, only two peaks, those occurring at m/z=11,688 and 11,829 Da exhibited decreases in intensity that were microcystin dose-dependent. The 11.8 kDa peak is consistent with β2-microglobulin, the light chain of the class J major histocompatibility antigens whose amino acid composition is similar among dogs, humans, mice, and rabbits, and is found free in biological fluids (Nakajima et al, 1999). The 11.8 kDa peak is also consistent with the molecular weight of murine serum amyloid A2 protein (SAA2; Yamamoto and Migita, 1985).…”

Section: Serum Protein Profilesmentioning

confidence: 55%

The toxicity of microcystin LR in mice following 7 days of inhalation exposure

Benson

Hutt

Rein

et al. 2005

Toxicon

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Microcystins, a family of cyclic heptapeptides produced by the cyanobacteria, Microcystis aeruginosa, have documented hepatotoxic and tumor promoting activities. The purpose of this study was to evaluate the toxicity of inhaled microcystin LR (microcystin). Male BALB/c mice were exposed by nose-only inhalation to 260-265 μg microcystin/m 3 for 7 days. The low-, mid-and highdose groups were exposed for 0.5, 1, and 2 h, respectively. Control animals were sham exposed to aerosolized vehicle. Treatment-related microscopic lesions were observed only in the nasal cavity of the mid-and high-dose groups. These lesions consisted of minimal to moderate multifocal degeneration and necrosis of the respiratory epithelium, with variable neutrophilic inflammation and minimal to marked degeneration, necrosis, and atrophy of the olfactory epithelium. The no-adverseeffect dose for the nasal lesions was approximately 3 μg/kg body weight, or 20 ng/cm 2 of nasal epithelium. In serum, only two protein peaks, occurring at m/zs of 11,688 and 11,829 Da, exhibited decreases in intensity that were microcystin dose-dependent. While these proteins have not been positively identified, they may be useful in the future as biomarkers of microcystin exposure in humans.

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The Complete Amino Acid Sequence of Dog β<sub>2</sub>-Microglobulin

Cited by 6 publications

References 28 publications

Selective quantitative bioanalysis of proteins in biological fluids by on-line immunoaffinity chromatography–protein digestion–liquid chromatography–mass spectrometry

Selective quantitative bioanalysis of proteins in biological fluids by on-line immunoaffinity chromatography–protein digestion–liquid chromatography–mass spectrometry

Determination of Canine .BETA.2-Microgloblin in Plasma and Urine by Enzyme-linked Immunosorbent Assay.

The toxicity of microcystin LR in mice following 7 days of inhalation exposure

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