The complete chloroplast genome of Euonymus szechuanensis

Abstract

The complete chloroplast genome from Euonymus szechuanensis, a rare deciduous shrub of the Celastraceae endemic to China, which is classified as vulnerable on the list of Threatened species in China because species diversity decreased sharply, is determined in this study. The whole chloroplast genome sequence of Euonymus szechuanensis has been characterized by Illumina pair-end sequencing. The circular genome is 157,465 bp long, containing a large single-copy region (LSC) of 86,401 bp and a small single-copy region (SSC) of 18,472 bp, which are separated by a pair of 26,296 bp inverted repeat regions (IRs). It encodes a total of 136 genes, including 84 protein-coding genes (76 PCG species), 44 tRNA genes (30 tRNA species), and 8 ribosomal RNA genes (4 rRNA species). Most of the gene species occur as a single copy, while 19 gene species occur in double copies. The overall A þ T content of the circular genome is 62.8%, while the corresponding values of the LSC, SSC and IR regions are 65.0, 68.4 and 57.3%, respectively. Phylogenetic analysis suggested that E. szechuanensis was relatively close to Euonymus schensianus among the species analyzed. This complete chloroplast genome will provide valuable insight into conservation and restoration efforts for this rare and Threatened species.

Characterization of the Complete Chloroplast Genome and Phylogenetic Implications of Euonymus microcarpus (Oliv.) Sprague

“…The GC content of the overall cpDNA is 37.22%, whereas that of the IR regions is higher than the LSC and SSC regions ( Figure 2 ). The same thing happened in the cpDNAs of E. fortunei , E. maackii , E. phellomanus , E. japonicus , E. schensianus , E. hamiltonianus and E. szechuanensis , respectively [ 14 , 29 ]. The GC content is treated as an indicator of the local recombination rate and stability of the secondary structures [ 30 ].…”

The complete chloroplast genome of Tripterygium wilfordii Hook. f. (Celastraceae)

“… 2017 ), Euonymus szechuanensis NC_047463 (Wang et al. 2020 ), Euonymus hamiltonianus NC_037518 (unpublished), Euonymus maackii NC_057059 (unpublished), Euonymus yunnanensis MW770452 (unpublished), Euonymus phellomanus NC_057060 (unpublished), Tripterygium wilfordii MN624264 (unpublished), Tripterygium regelii MN624266 (unpublished), Tripterygium hypoglaucum MN624265 (unpublished), Celastrus orbiculatus MW316708 (unpublished), Celastrus aculeatus MW801026 (unpublished), Celastrus paniculatus OL804289 (unpublished), Catha edulis KT861471 (unpublished), Maytenus guangxiensis NC_047301 (Shi and Liu 2020 ), Salacia amplifolia NC_047214 (Lin et al. 2019 ), Monimopetalum chinense MK450440 (Pan et al.…”

Disjunction and Vicariance Between East and West Asia: A Case Study on Euonymus sect. Uniloculares Based on Plastid Genome Analysis

“…As a result, species identifications are disputed, and taxonomists are currently attempting to resolve the debate using genetic data. Wang et al (2020) constructed phylogenetic trees based on whole cp genomes, in which E. schensianus and E. szechuanensis were grouped together in a clade with 100% resolution. E. japonicus was grouped in a subclade with the latter, closely approximating our results.…”