The complete chloroplast genome sequence of <i>Erigeron breviscapus</i> and <i>Erigeron multiradiatus</i> (Asteraceae)

Li, Zhong-Ji; Liu, Yingying; Yang, Cong-Wei; Zhou, Qian; Li, Guodong

doi:10.1080/23802359.2019.1683478

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…The following sequences were used: Solidago altissima PP108063 (present study), Erigeron canadensis MT806101 (Oh and Park 2023 ), Dichrocephala benthamii ON751565 (unpublished), E. annuus OL350834 (unpublished), Aster ageratoides MW813970 (unpublished), Symphyotrichum subulatum MN541093 (Hu 2020 ), E. multiradiatus NC056169 (Li et al. 2019 ), E. breviscapus MN449489 (Li et al. 2019 ), Eclipta Alba MF993496 (Kim et al.…”

Section: Resultsmentioning

confidence: 99%

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The complete chloroplast genome assembly of Solidago altissima (Lineaus, 1753) (Astereae; Asteraceae)

Kang,

Son,

Kim

2024

Mitochondrial DNA Part B

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This study aims to report the complete chloroplast genome of Solidago altissima L., a globally recognized invasive plant. The complete genome length of S. altissima is 152,961 bp; S. altissima has a typical quadripartite structure (including a large single copy of 84,829 bp, a small single copy of 18,084 bp, and two inverted repeat regions of 25,024 bp), which is commonly found in angiosperms. The genome contains 129 genes, consisting of 85 coding sequences, 36 tRNA genes, and 8 rRNA genes. To understand the phylogenetic relationship between S. altissima and its related species, maximum likelihood analysis was performed. The results revealed that S. altissima is closely related to Symphyotrichum subulatum . The findings of the present study could provide fundamental data for the future phylogenetic and evolutionary studies, while also research on species invasion and resolving complexity of S. altissima .

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Section: Resultsmentioning

confidence: 99%

“… 2019 ), E. breviscapus MN449489 (Li et al. 2019 ), Eclipta Alba MF993496 (Kim et al. 2017 ), Lagenophora cuchumatanica KX063879 (Vargas et al.…”

Section: Resultsmentioning

confidence: 99%

The complete chloroplast genome assembly of Solidago altissima (Lineaus, 1753) (Astereae; Asteraceae)

Kang,

Son,

Kim

2024

Mitochondrial DNA Part B

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“…While complete plastome sequences for certain plant groups have recently been generated as genomic references and for phylogenomic investigations at various taxonomic levels [ 17 , 19 , 22 , 23 , 24 , 25 ], very little is known about the plastomic genomes reference of Erigeron and related genera, including Conyza [ 26 , 27 ]. In particular, the plastomes of tribe Astereae from the Southern Hemisphere, including subtribe Conyzinae, are poorly represented, and both specific and higher-level relationships in Erigeron are poorly understood [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Chloroplast Genome Provides Insights into Molecular Evolution and Species Relationship of Fleabanes (Erigeron: Tribe Astereae, Asteraceae) in the Juan Fernández Islands, Chile

Kim,

Yang,

Cho

et al. 2024

Plants

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Erigeron represents the third largest genus on the Juan Fernández Islands, with six endemic species, five of which occur exclusively on the younger Alejandro Selkirk Island with one species on both islands. While its continental sister species is unknown, Erigeron on the Juan Fernández Islands appears to be monophyletic and most likely evolved from South American progenitor species. We characterized the complete chloroplast genomes of five Erigeron species, including accessions of E. fernandezia and one each from Alejandro Selkirk and Robinson Crusoe Islands, with the purposes of elucidating molecular evolution and phylogenetic relationships. We found highly conserved chloroplast genomes in size, gene order and contents, and further identified several mutation hotspot regions. In addition, we found two positively selected chloroplast genes (ccsA and ndhF) among species in the islands. The complete plastome sequences confirmed the monophyly of Erigeron in the islands and corroborated previous phylogenetic relationships among species. New findings in the current study include (1) two major lineages, E. turricola–E. luteoviridis and E. fernandezia–E. ingae–E. rupicola, (2) the non-monophyly of E. fernandezia occurring on the two islands, and (3) the non-monophyly of the alpine species E. ingae complex.

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“…However, with the in-depth study of E. breviscapus chloroplasts, the current research on the genetic background of this species mainly focuses on the use of the chloroplast genome of E. breviscapus for phylogenetic research and germplasm resource protection ( Li et al, 2019 ; Meng et al, 2019 ), Meng et al (2019) used the Illumina sequencing platform to obtain a chloroplast genome with a size of 152,183bp, but the article only briefly mentioned the basic structural information of the species’s chloroplast genome and the type and number of genes, and the phylogenetic position of E. breviscapus was determined. Li et al (2019) used the Illumina sequencing platform to obtain a chloroplast genome with a size of 152,367bp, and only briefly introduced the basic structural information of the species’s chloroplast genome and the type and number of genes in the article, and the phylogenetic analysis was conducted to infer phylogenetic position of E. breviscapus and Erigeron multiradiatus within the family of Asteraceae, the above two reports did not mention the test data and its quality control. Both of the above mentioned studies lacked systematic prediction of the gene structure of the chloroplast genome of E. breviscapus and bioinformatics analysis such as codon usage bias analysis and simple sequence repeat (SSR) analysis.…”

Section: Introductionmentioning

confidence: 99%

Complete Chloroplast Genome Sequence of Erigeron breviscapus and Characterization of Chloroplast Regulatory Elements

Ouyang

Guo

et al. 2021

Front. Plant Sci.

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Erigeron breviscapus is a famous medicinal plant. However, the limited chloroplast genome information of E. breviscapus, especially for the chloroplast DNA sequence resources, has hindered the study of E. breviscapus chloroplast genome transformation. Here, the complete chloroplast (cp) genome of E. breviscapus was reported. This genome was 152,164bp in length, included 37.2% GC content and was structurally arranged into two 24,699bp inverted repeats (IRs) and two single-copy areas. The sizes of the large single-copy region and the small single-copy region were 84,657 and 18,109bp, respectively. The E. breviscapus cp genome consisted of 127 coding genes, including 83 protein coding genes, 36 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. For those genes, 95 genes were single copy genes and 16 genes were duplicated in two inverted regions with seven tRNAs, four rRNAs, and five protein coding genes. Then, genomic DNA of E. breviscapus was used as a template, and the endogenous 5' and 3' flanking sequences of the trnI gene and trnA gene were selected as homologous recombinant fragments in vector construction and cloned through PCR. The endogenous 5' flanking sequences of the psbA gene and rrn16S gene, the endogenous 3' flanking sequences of the psbA gene, rbcL gene, and rps16 gene and one sequence element from the psbN-psbH chloroplast operon were cloned, and certain chloroplast regulatory elements were identified. Two homologous recombination fragments and all of these elements were constructed into the cloning vector pBluescript SK (+) to yield a series of chloroplast expression vectors, which harbored the reporter gene EGFP and the selectable marker aadA gene. After identification, the chloroplast expression vectors were transformed into Escherichia coli and the function of predicted regulatory elements was confirmed by a spectinomycin resistance test and fluorescence intensity measurement. The results indicated that aadA gene and EGFP gene were efficiently expressed under the regulation of predicted regulatory elements and the chloroplast expression vector had been successfully constructed, thereby providing a solid foundation for establishing subsequent E. breviscapus chloroplast transformation system and genetic improvement of E. breviscapus.

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The complete chloroplast genome sequence of Erigeron breviscapus and Erigeron multiradiatus (Asteraceae)

Cited by 4 publications

References 8 publications

The complete chloroplast genome assembly of Solidago altissima (Lineaus, 1753) (Astereae; Asteraceae)

The complete chloroplast genome assembly of Solidago altissima (Lineaus, 1753) (Astereae; Asteraceae)

Chloroplast Genome Provides Insights into Molecular Evolution and Species Relationship of Fleabanes (Erigeron: Tribe Astereae, Asteraceae) in the Juan Fernández Islands, Chile

Complete Chloroplast Genome Sequence of Erigeron breviscapus and Characterization of Chloroplast Regulatory Elements

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