The complete genome of the tospovirus Zucchini lethal chlorosis virus

Abstract

BackgroundZucchini lethal chlorosis virus (ZLCV) causes significant losses in the production of cucurbits in Brazil. This virus belongs to the genus Tospovirus (family Bunyaviridae) and seems to be exclusively transmitted by Frankliniella zucchini (Thysanoptera). Tospoviruses have a tripartite and single-stranded RNA genome classified as S (Small), M (Medium) and L (Large) RNAS. Although ZLCV was identified as a member of the genus Tospovirus in 1999, its complete genome had not been sequenced until now.FindingsWe sequenced the full-length genome of two ZLCV isolates named ZLCV-SP and ZLCV-DF. The phylogenetic analysis showed that ZLCV-SP and ZLCV-DF clustered with the previously reported isolate ZLCV-BR09. Their proteins were closely related, except the non-structural protein (NSm), which was highly divergent (approximately 90 % identity). All viral proteins clustered similarly in our phylogenetic analysis, excluding that these ZLCV isolates have originated from reassortment events of different tospovirus species.ConclusionHere we report for the first time the complete genome of two ZLCV isolates that were found in the field infecting zucchini and cucumber.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0577-4) contains supplementary material, which is available to authorized users.

The Sw-5 Gene Cluster: Tomato Breeding and Research Toward Orthotospovirus Disease Control

“…Besides the aforementioned four orthotospoviruses, Sw-5b provided resistance to alstroemeria necrotic streak virus (ANSV) and impatiens necrotic spot virus (INSV) as well ( Leastro et al, 2017 ). Thus, the Sw-5b gene alone is sufficient for broad-spectrum resistance, although this applies to phylogenetically related orthotospoviruses that are clustered in the same evolutionary clade ( de Oliveira et al, 2012 ; Lima et al, 2016 ). Conversely, viruses phylogenetically distant from this “TSWV clade,” e.g., melon yellow spot virus (MYSV), are not sensed and overcome the resistance when mechanically inoculated onto these Sw-5b gene-transformed N-benthamiana plants ( Hallwass et al, 2014 ).…”

Discovering and sequencing new plant viral genomes by next‐generation sequencing: description of a practical pipeline

“…Using this method, the complete sequence of the S, M and L segments of the Zucchini lethal chlorosis virus (ZLCV, accession numbers KU681010‐KU681012), a tospovirus, was determined and recently published by Lima et al . ().…”

Multiplex RT-PCR identification of five viruses associated with the watermelon crops in the Brazilian Cerrado

“…The most important viruses that affect the watermelon plants are: (i) cucumovirus as Cucumber mosaic virus (CMV) (Roossinck, 2001); (ii) potyviruses as Papaya ringspot virus type W (PRSV-W) (Barnett, 1991;Bateson et al, 2002), Watermelon mosaic virus (WMV) (Desbiez and Lecoq, 2004;Purcifull and Hiebert, 1979), and Zucchini yellow mosaic virus (ZYMV) (Desbiez and Lecoq, 1997;Harth et al, 2017); and (iii) orthotospovirus as Zucchini lethal chlorosis viruses (ZLCV) (Camelo-García et al, 2015;Lima et al, 2016). These viruses are widely distributed in the cucurbit cultivation areas in Brazil, limiting the yield and fruit quality (Aguiar et al, 2013(Aguiar et al, , 2015Soares et al, 2016).…”