The complete mitochondrial genome of Cherskii’s sculpin ( Cottus czerskii ) (Scorpaeniformes: Cottidae)

Abstract

The complete mitogenome sequence of Cherskii's sculpin (Cottus czerskii) is determined using long PCR. The genome is 16,534 bp in length and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. The overall base composition of the heavy strand is A (26.4%), C (30.1%), T (26.1%) and G (17.5%). The control region is 857 bp in length and the A + T content of the region is 61.5%. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of Cherskii's sculpin. Mitochondrial genome analyses based on MP, NJ and Bayesian analyses yielded identical phylogenetic trees, indicating a close phylogenetic affinity of the 21 Actinopterygii species. It appears that no less than two major phyletic lineages were present in Actinopterygii. The main clades within the Perciformes and Scorpaeniformes supported are: a clade including the Scombridae, Kyphosidae and Percichthyidae; a clade (Cottidae) with the Trichodontidae as the sister taxon to Pholidae, Anarhichadidae and Zoarcidae, which was supported by bootstrap values of 92%. The five Cottus species formed a paraphyletic group with the high bootstrap value (100%) in all examinations.

Complete mitochondrial genomes forCottus asper,Cottus perifretum, andCottus rhenanus(Perciformes, Cottidae)

“…Minimum evolution and neighbour-joining trees resulted in the same tree topology as the maximum likelihood tree. With the exception of the taxa added in this paper, phylogenetic analyses revealed the same tree topologies (Figure 1) reported in recent studies of Cottus mitochondrial genomes (Balakirev et al 2016;Han et al 2016;Swanburg et al 2016). Despite limited taxonomic coverage, this new Cottus topology suggests multiple independent invasions of Asia, Europe, and North America (Figure 1).…”

Complete mitochondrial genomes of Baikal oilfishes (Perciformes: Cottoidei), earth’s deepest-swimming freshwater fishes

“…Minimum evolution and neighbor-joining trees resulted in the same tree topology as the maximum likelihood tree. With the exception of the taxa added in this paper, phylogenetic analyses revealed the same tree topologies reported in recent studies of Cottus mitochondrial genomes (Balakirev et al 2016; Han et al 2016; Swanburg et al 2016; Fast et al 2017). …”

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“…The structure and characteristic of complete mitochondrial genome of C. dzungaricus was circular with a similar number and arrangement of genes as other reported sculpins (Hwang, Byeon, & Lee, 2013; Han , Li, Zhao, & Xu, 2016;Balakirev, Saveliev, & Ayala, 2016;Fast, Aguilar, Nolte, & Sandel, 2017), but slightly different from the sequence (Genbank no. KM093860) uploaded into NCBI database (Ao et al, 2014), which was obtained by the conventional Sanger dideoxy sequencing method.…”