The complete nucleotide sequence of cucumber green mottle mosaic virus (SH strain) genomic RNA

Ugaki, Masashi; Tomiyama, Masamitsu; Kakutani, Tetsuji; Hidaka, Soh; Kiguchi, T.; Nagata, Ryuji; Satô, Takeshi; Motoyoshi, Fusao; Nishiguchi, Masamichi

doi:10.1099/0022-1317-72-7-1487

Cited by 111 publications

(64 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This hypothesis was proposed on the basis of immunochemical analyses of the colonization of rice plants by Rice yellow mottle virus (Opalka et al, 1998) and on the well-known role of calcium in the stabilization of Sobemovirus isometric capsids (Hsu et al, 1976;Opalka et al, 2000). It is known that calcium binding stabilizes TMV particles (Gallagher & Lauffer, 1983;Namba et al, 1989), and putative calcium binding sites have been described for CGMMV, watermelon strain (Wang & Stubbs, 1994), which has the same amino acid sequence in the CP as the SH strain (Ugaki et al, 1991). We could speculate that the higher efficiency of xylem transport of CGMMV at 29 versus 24 uC could be related to a greater virus accumulation and, hence, higher calcium binding in the xylem and/or to relaxation of the pit membrane at the higher temperature.…”

Section: Discussionmentioning

confidence: 99%

“…CGMMV strain SH (Ugaki et al, 1991) was grown in cucumber plants in the greenhouse and purified according to Fukuda et al (1981). Plants were inoculated in the adaxial surface of cotyledons or leaves with CGMMV particles at a final concentration of 300 mg ml 21 in 0?1 M Na 2 HPO 4 , pH 7?0.…”

Section: Methodsmentioning

confidence: 99%

“…Hence, systemic movement of plant viruses through the xylem is a relatively unexplored process. Further studies are needed for a better knowledge of the occurrence of xylem transport in different viral taxa and to analyse the mechanism of xylem transport of plant viruses and its relevance compared with systemic movement through the phloem.Cucumber green mottle mosaic virus (CGMMV) is a Tobamovirus that shares the same genetic organization and similar particle morphology as Tobacco mosaic virus (TMV) (Fukuda et al, 1981;Namba et al, 1989;Ugaki et al, 1991;Wang & Stubbs, 1994). The systemic transport of CGMMV has been analysed in our laboratory and was shown to circulate as virus particles in the phloem of infected cucumber plants (Cucumis sativus L.) (Simó n-Buela & García-Arenal, 1999).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus

Moreno

Thompson

García‐Arenal

2004

Journal of General Virology

View full text Add to dashboard Cite

Systemic movement of Cucumber green mottle mosaic virus (CGMMV) in cucumber plants was shown to be from photoassimilate source to sink, thus indicating phloem transport. Nevertheless, CGMMV was not detected by immunocytochemical procedures in the intermediary cell-sieve element complex in inoculated cotyledons, where photoassimilate loading occurs. In stem internodes, CGMMV was first localized in the companion cells of the external phloem and subsequently in all tissues except the medulla, therefore suggesting leakage of the virus from, and reloading into, the transport phloem during systemic movement. In systemically infected sink leaves, CGMMV was simultaneously detected in the xylem and phloem. Interestingly, CGMMV accumulated to high levels in the differentiating tracheids of young leaves implying that the xylem could be involved in the systemic movement of CGMMV. This possibility was tested using plants in which cell death was induced in a portion of the stem by steam treatment. At 24 6C, steam treatment effectively prevented the systemic movement of CGMMV, even though viral RNA was detected in washes of the xylem above the steamed internode suggesting that xylem circulation occurred. At 29 6C, CGMMV systemically infected steam-treated cucumber plants, indicating that CGMMV can move systemically via the xylem. Xylem transport of CGMMV was, however, less efficient than phloem transport in terms of the time required for systemic infection and the percentage of plants infected. INTRODUCTIONSystemic transport through the vascular system is a crucial step in plant virus infection. Most plant viruses are thought to move systemically through the phloem in parallel with photoassimilate transport (reviewed by Haywood et al., 2002;Lucas & Wolf, 1999;Nelson & Van Bel, 1998;Oparka & Turgeon, 1999;Thompson & Schulz, 1999). Phloem transport includes the loading (entry) of the virus into the phloem at source tissues, its circulation in the transport phloem and its unloading (exit) from the phloem at the sink tissues. Photoassimilate loading occurs presumably in the minor veins of source organs (Nelson & Van Bel, 1998;Santa Cruz, 1999) and in the case of cucurbits is thought to follow a symplastic route involving the specialized intermediary cell-sieve element complex (Turgeon, 1996). Knowledge of phloem circulation of plant viruses is far from complete, yet it is known that it parallels photoassimilate flow (Leisner et al., 1992;Oparka & Turgeon, 1999). The requirement of a functional capsid protein (CP) for systemic movement is common but not universal, depending on specific virus-host interactions (e.g. Dalmay et al., 1992;McGeachy & Barker, 2000;Petty & Jackson, 1990;Ryabov et al., 2001), and for most viruses the precise form in which the virus is transported has not been determined. Phloem unloading in the sink organs also parallels photoassimilate unloading and proceeds basipetally in the developing leaf during the sink-to-source transition (Cheng et al., 2000;Leisner et al., 1992; Roberts et al., 1997; Santa Cruz e...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus

Moreno

Thompson

García‐Arenal

2004

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…Thereafter reports were made in USA and Canada (Tian et al, 2014;Ling et al, 2014); Saudi Arabia (Al-Shahwan and Abdalla, 1992; Al-Saleh and Al- Shahwan, 1997); Iran (Moradi and Jafarpour, 2011), China and Greece (Zhang et al, 2009;Varveri et al, 2002), Korea and Myammar (Yoon et al, 2008;Kim et al, 2010), and in the Ukraine (Budzanivska et al, 2007). Several strains of CGMMV have been reported from Europe, Pakistan, India and Japan (Komuru et al, 1968; Lee, 1996;Antignus et al, 1990;Francki et al, 1986;Ugaki et al, 1991;Shim et al, 2005;Ko et al, 2006). CGMMV is mechanically and seed transmitted with a narrow host range comprising of cucurbits only and is different in host range from Tobacco mosaic virus (TMV) whose main hosts are members of the solanaceae (Antignus et al, 1990(Antignus et al, , 2001.…”

Section: Introductionmentioning

confidence: 99%

Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

Amer¹

2015

IJAB

View full text Add to dashboard Cite

To cite this paper: Amer, M.A., 2015. Biological and molecular characterization of cucumber green mottle mosaic virus affecting bottle gourd and watermelon plants in Saudi Arabia. AbstractSamples were collected from seven of each bottle gourd and watermelon plants grown in Riyadh and Hail regions of Saudi Arabia in May, 2012 showing symptom suspected to be virus infection and analyzed by a double antibody sandwich against bottle gourd and watermelon viruses. Two of watermelon and three of bottle gourd samples were found to be positive with Cucumber green mottle mosaic virus (CGMMV). However, variable results were obtained with other viruses infecting cucurbits. One sample of CGMMV positive samples from each region were homogenized in a mortar separately and used to make mechanical inoculation on 25 plant species which produced characteristic symptoms of tobamo virus infection. Five ELISA-positive samples from naturally infection were further tested and detected by RT-PCR assay using virus specific primers that amplified a 400 bp fragment in the coat protein gene. The nucleotide sequence of the PCR products from the Saudi isolates of CGMMV isolated from bottle gourd and watermelon were determined and demonstrated its similarity to the other isolates of CGMMV recorded in NCBI. The CP gene of CGMMV-Saudi Arabia isolates shared between 87.2% and 99.7% identity in nucleotide level. The synthesized cDNA probe for CGMMV detection was hybridized with nucleic acid extracts from infected samples collected from the above mentioned locations. This is the first report regarding the genetic makeup of CGMMV at the molecular level in Saudi Arabia.

show abstract

“…Crop yield losses of about 15% have been reported (1,2). CGMMV contains a 6.4 kb (+) ssRNA genome (6). The virions are rod-shaped, approximately 300 nm in length and 18 nm in diameter (5).…”

mentioning

confidence: 99%

First report of cucumber green mottle mosaic virus association with the leaf green mosaic disease of a vegetable crop, Luffa acutangula L.

Sharma¹,

Verma²,

Mishra³

et al. 2014

View full text Add to dashboard Cite

The complete nucleotide sequence of cucumber green mottle mosaic virus (SH strain) genomic RNA

Cited by 111 publications

References 37 publications

Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus

Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus

Biological and Molecular Characterization of Cucumber green mottle mosaic virus Affecting Bottle Gourd and Watermelon Plants in Saudi Arabia

First report of cucumber green mottle mosaic virus association with the leaf green mosaic disease of a vegetable crop, Luffa acutangula L.

Contact Info

Product

Resources

About