The complete sequence of the mitochondrial genome of <i>Saccharomyces cerevisiae</i>

Foury, Françoise; Roganti, Tiziana; Lecrenier, Nicolas; Purnelle, Bénédicte

doi:10.1016/s0014-5793(98)01467-7

Cited by 405 publications

(424 citation statements)

References 35 publications

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“…In two clones we found the 21S rRNA at one extremity of the insert and the 15S rRNA at the other extremity. This shows that the 15S rRNA and 21S rRNA genes map close to each other (about 2 kb) on the mitochondrial map of S. kluyveri, in contrast to S. cerevisiae, in which at least 30.5 kb separate 15S from 21S rDNA [20]. The COX1 gene was identi¢ed in three RSTs.…”

Section: Repeated Sequences and Extrachromosomal Sequencesmentioning

confidence: 79%

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Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

et al. 2000

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The genome of Saccharomyces kluyveri was explored through 2528 random sequence tags with an average length of 981 bp. The complete nuclear ribosomal DNA unit was found to be 8656 bp in length. Sequences homologous to retroelements of the gypsy and copia types were identified as well as numerous solo long terminal repeats. We identified at least 1406 genes homologous to Saccharomyces cerevisiae open reading frames, with on average 58.1% and 72.4% amino acid identity and similarity, respectively. In addition, by comparison with completely sequenced genomes and the SwissProt database, we found 27 novel S. kluyveri genes. Most of these genes belong to pathways or have functions absent from S. cerevisiae, such as the catabolic pathway of purines or pyrimidines, melibiose fermentation, sorbitol utilization, or degradation of pollutants. The sequences are deposited in EMBL under the accession numbers AL404849^AL407376. ß

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Section: Repeated Sequences and Extrachromosomal Sequencesmentioning

confidence: 79%

“…When we started this work, only 46 S. kluyveri DNA sequences were available in GenBank, most of them corresponding to ribosomal RNA sequences (16) and a variety of nuclear genes (20). Only four of these sequences correspond to mitochondrial genes (COX3, COX2 and ATP8) and three to the pSKL plasmid.…”

Section: Introductionmentioning

confidence: 99%

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

et al. 2000

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“…The mitochondrial DNA of K. thermotolerans was previously characterized by restriction mapping [7]. With only 23 kb it is much smaller than the 86 kb S. cerevisiae mitochondrial genome [15]. We have found only 28 RSTs corresponding to mitochondrial DNA, 22 of them form seven small contigs.…”

Section: Mitochondrial Dnamentioning

confidence: 79%

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

et al. 2000

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A genomic exploration of Kluyveromyces thermotolerans was performed by random sequence tag (RST) analysis. We sequenced 2653 RSTs corresponding to inserts sequenced from both ends. We performed a systematic comparison with a complete set of proteins from Saccharomyces cerevisiae, other completely sequenced genomes and SwissProt. We identified six mitochondrial genes and 1358^1496 nuclear genes by comparison with S. cerevisiae. In addition, 25 genes were identified by comparison with other organisms. This corresponds to about 24% of the estimated gene content of this organism. A lower level of conservation is observed with orthologues to genes of S. cerevisiae previously classified as orphans. Gene order was found to be conserved between S. cerevisiae and K. thermotolerans in 56.5% of studied cases. ß

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“…First, a series of seven S. cerevisiae data ¢les comprising: (1) the 6213 predicted protein sequences as described in [1]; (2) the intergenic and subtelomeric DNA sequences [4]; (3) the 2 Wm plasmid DNA sequence [8]; (4) the rDNA sequences extracted from the chromosome XII sequence [9]; (5) the 52 distinct tRNA gene sequences as de¢ned in [1]; (6) the Ty elements DNA sequences [10]; and (7) the mitochondrial DNA sequence [11] and its translation products.…”

Section: Databasesmentioning

confidence: 99%

Genomic Exploration of the Hemiascomycetous Yeasts: 3. Methods and strategies used for sequence analysis and annotation

et al. 2000

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The primary analysis of the sequences for our Hemiascomycete random sequence tag (RST) project was performed using a combination of classical methods for sequence comparison and contig assembly, and of specifically written scripts and computer visualization routines. Comparisons were performed first against DNA and protein sequences from Saccharomyces cerevisiae, then against protein sequences from other completely sequenced organisms and, finally, against protein sequences from all other organisms. Blast alignments were individually inspected to help recognize genes within our random genomic sequences despite the fact that only parts of them were available. For each yeast species, validated alignments were used to infer the proper genetic code, to determine codon usage preferences and to calculate their degree of sequence divergence with S. cerevisiae. The quality of each genomic library was monitored from contig analysis of the DNA sequences. Annotated sequences were submitted to the EMBL database, and the general annotation tables produced served as a basis for our comparative description of the evolution, redundancy and function of the Hemiascomycete genomes described in other articles of this issue. ß

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The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae

Cited by 405 publications

References 35 publications

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

Genomic Exploration of the Hemiascomycetous Yeasts: 9. Saccharomyces kluyveri

Genomic Exploration of the Hemiascomycetous Yeasts: 10.Kluyveromyces thermotolerans

Genomic Exploration of the Hemiascomycetous Yeasts: 3. Methods and strategies used for sequence analysis and annotation

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