The complete unique long sequence and the overall genomic organization of the GA strain of Marek's disease virus

Lee, Lucy F.; Wu, Ping; Sui, Dexin; Ren, Delin; Kamil, Jeremy P.; Kung, Hsing Jien; Witter, R. L.

doi:10.1073/pnas.97.11.6091

Cited by 188 publications

(155 citation statements)

References 44 publications

Supporting

Mentioning

150

Contrasting

Unclassified

Order By: Relevance

“…1 and vIL-8 no. 4 (Table 1), which correspond to nucleotides (nt) 848 to 872 and the reverse complement of nt 1762 to 1786 of the BamHI-L fragment of the MDV genome, inclusive (31). The resulting PCR product is therefore 939 bp using the parental (RB1B) virus as template and 1948 bp using the mutant (RB1BvIL-8⌬smGFP) virus as template, due to vIL-8 deletion and expression cassette insertion.…”

Section: Cells and Viruses Msb-1 Cells (3)mentioning

confidence: 99%

Marek's Disease Virus (MDV) Encodes an Interleukin-8 Homolog (vIL-8): Characterization of the vIL-8 Protein and a vIL-8 Deletion Mutant MDV

Parcells

Lin

Dienglewicz

et al. 2001

J Virol

Self Cite

155

134

View full text Add to dashboard Cite

Chemokines induce chemotaxis, cell migration, and inflammatory responses. We report the identification of an interleukin-8 (IL-8) homolog, termed vIL-8, encoded within the genome of Marek's disease virus (MDV).The 134-amino-acid vIL-8 shares closest homology to mammalian and avian IL-8, molecules representing the prototype CXC chemokine. The gene for vIL-8 consists of three exons which map to the BamHI-L fragment within the repeats flanking the unique long region of the MDV genome. A 0.7-kb transcript encoding vIL-8 was detected in an n-butyrate-treated, MDV-transformed T-lymphoblastoid cell line, MSB-1. This induction is essentially abolished by cycloheximide and herpesvirus DNA polymerase inhibitor phosphonoacetate, indicating that vIL-8 is expressed with true late (␥ 2 ) kinetics. Baculovirus-expressed vIL-8 was found to be secreted into the medium and shown to be functional as a chemoattractant for chicken peripheral blood mononuclear cells but not for heterophils. To characterize the function of vIL-8 with respect to MDV infection in vivo, a recombinant MDV was constructed with a deletion of all three exons and a soluble-modified green fluorescent protein (smGFP) expression cassette inserted at the site of deletion. In two in vivo experiments, the vIL-8 deletion mutant (RB1BvIL-8⌬smGFP) showed a decreased level of lytic infection in comparison to its parent virus, an equal-passage-level parent virus, and to another recombinant MDV containing the insertion of a GFP expression cassette at the nonessential US2 gene. RB1BvIL-8⌬smGFP retained oncogenicity, albeit at a greatly reduced level. Nonetheless, we have been able to establish a lymphoblastoid cell line from an RB1BvIL-8⌬smGFP-induced ovarian lymphoma (MDCC-UA20) and verify the presence of a latent MDV genome lacking vIL-8. Taken together, these data describe the identification and characterization of a chemokine homolog encoded within the MDV genome that is dispensable for transformation but may affect the level of MDV in vivo lytic infection.

show abstract

Section: Cells and Viruses Msb-1 Cells (3)mentioning

confidence: 99%

Marek's Disease Virus (MDV) Encodes an Interleukin-8 Homolog (vIL-8): Characterization of the vIL-8 Protein and a vIL-8 Deletion Mutant MDV

Parcells

Lin

Dienglewicz

et al. 2001

J Virol

Self Cite

155

134

View full text Add to dashboard Cite

show abstract

“…do not in general encode chemokines, chemokine receptors or chemokine binding proteins; however, Marek's disease virus (a lymphotropic chicken a-herpesvirus) encodes an IL-8/CXCL8 like CXC chemokine, that until now remains uncharacterized (Lee et al, 2000).…”

Section: Virally Encoded Chemokines and Chemokine Receptorsmentioning

confidence: 99%

Virally encoded 7TM receptors

Rosenkilde¹,

Waldhoer²,

Lüttichau

et al. 2001

Oncogene

View full text Add to dashboard Cite

“…Horizontal spread, thus far, is the only property lacking in rRB-1B to make it a full model to study MDV biology. Despite this shortcoming, pRB-1B and its derivatives are great tools to determine the molecular determinants involved in MDV horizontal dissemination in birds by individual, consequential or concomitant repair of genes that are found to be different from authentic, wild-type MDV [17,32].…”

Section: Introductionmentioning

confidence: 99%

A full UL13 open reading frame in Marek's disease virus (MDV) is dispensable for tumor formation and feather follicle tropism and cannot restore horizontal virus transmission of rRB-1B in vivo

et al. 2007

View full text Add to dashboard Cite

-Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that is highly contagious in poultry. Recombinant RB-1B (rRB-1B) reconstituted from an infectious genome cloned as a bacterial artificial chromosome (BAC) is unable to spread horizontally, quite in contrast to parental RB-1B. This finding suggests the presence of one or several mutations in cloned relative to parental viral DNA. Sequence analyses of the pRB-1B bacmid identified a one-nucleotide insertion in the UL13 orthologous gene that causes a frame-shift mutation and thereby results in a theoretical truncated UL13 protein (176 aa vs. 513 aa in parental RB-1B). UL13 genes are conserved among alphaherpesviruses and encode protein kinases. Using two-step "en passant" mutagenesis, we restored the UL13 ORF in pRB-1B. After transfection of UL13-positive pRB-1B DNA (pRB-1B*UL13), the resulting, repaired virus did not exhibit a difference in cell-to cell spread (measured by plaque sizes) and in UL13 transcripts in culture compared to parental rRB-1B virus. Although 89% of the chickens inoculated with rRB-1B*UL13 virus developed tumors in visceral organs, none of the contact birds did. MDV antigens were clearly expressed in the feather tips of rRB-1B infected chickens, suggesting that the UL13 gene mutation did not alter virus tropism of the feather follicle. The results indicate that the correction in UL13 gene alone is not sufficient to restore in vivo spreading capabilities of the rRB-1B virus, and that other region(s) of pRB-1B might be involved in the loss-of-function phenotype. This finding also shows for the first time that a full UL13 ORF is dispensable for MDV tumor formation and feather follicle tropism.Marek's disease virus / UL13 / pathogenesis / horizontal spread / chicken

show abstract

The complete unique long sequence and the overall genomic organization of the GA strain of Marek's disease virus

Cited by 188 publications

References 44 publications

Marek's Disease Virus (MDV) Encodes an Interleukin-8 Homolog (vIL-8): Characterization of the vIL-8 Protein and a vIL-8 Deletion Mutant MDV

Marek's Disease Virus (MDV) Encodes an Interleukin-8 Homolog (vIL-8): Characterization of the vIL-8 Protein and a vIL-8 Deletion Mutant MDV

Virally encoded 7TM receptors

A full UL13 open reading frame in Marek's disease virus (MDV) is dispensable for tumor formation and feather follicle tropism and cannot restore horizontal virus transmission of rRB-1B in vivo

Contact Info

Product

Resources

About