The complexities of proanthocyanidin biosynthesis and its regulation in plants

Yu, Keji; Song, Yushuang; Lin, Jun; Dixon, Richard A.

doi:10.1016/j.xplc.2022.100498

Cited by 47 publications

(38 citation statements)

References 176 publications

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“…The abundance of PAs and anthocyanin has been determined based on the competition between some parallel pathways. 1,5 The mechanisms of PA and anthocyanin biosynthesis, which are coregulated by structural genes and transcription factors, have been well studied at the transcriptional level in various plant species. 1,5 However, research on the post-transcriptional regulation of PA biosynthesis in cotton is relatively limited.…”

Section: Discussionmentioning

confidence: 99%

“…PA and anthocyanin content were determined by competition between parallel flavonoid pathways. 1 Our previous study showed that the empurpled mutant HS2, which was induced by a T-DNA insertion in the parental line C312, enhances anthocyanidin biosynthesis and accumulation. 39 After analyzing the omics data deposited in Sequence Read Archive (SRA) of the NCBI under the accession numbers PRJNA663418 from C312 and HS2, we selected GhmiR858, a conserved miRNA, which plays an influential role in flavonoid biosynthesis in plants, for further investigations (Figures 1A, S1, and Table S1).…”

Section: Ghmir858 Is Highly Expressed In a G Hirsutummentioning

confidence: 99%

“…ANR is the rate-limiting enzyme responsible for the conversion of anthocyanidins and their substrates to PAs. 1,5 It has been reported that MYB transcription factors typically bind to MYB binding sites (MBSs) to regulate the transcription of target genes. 43 As expected, the sequence in the GhANR (Gh_A05G152700) promoter contains three MBSs: AACCTAA, CAACTG, and CAGTTG.…”

Section: Ghtt2l Induces the Activities Of Ghanr Through Binding To Itsmentioning

confidence: 99%

“…Proanthocyanidins (PAs), a kind of flavonoid whose biosynthesis begins from the central phenylpropanoid pathway, are ubiquitous oligomeric or polymeric flavan-3-ols. 1 As important secondary metabolites, PAs commonly accumulate in different tissues and organs of cash crops, such as those of the seed coat, leaf, and fiber. 2 PAs are the second most abundant phenolic polymers in plants and are vital for the regulation of growth and development, as well as for certain biotic and abiotic stress responses.…”

Section: Introductionmentioning

confidence: 99%

“…1,5 For instance, anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) are key rate-limiting enzymes responsible for the conversion of anthocyanins into the structural and initiation units of PAs. 1 MYB transcription factor TT2 interacts with transcription factors TT8 and TTG1 to regulate PA biosynthesis by activating the expression of ANR in the Arabidopsis seed coat. 6 The coexpressed MYB115 and TTG1 directly bind to the promoter of ANR1, a structural gene in the biosynthesis of PAs, to induce its expression, leading to accumulation of PAs in poplar.…”

Section: Introductionmentioning

confidence: 99%

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GhmiR858 Inhibits the Accumulation of Proanthocyanidins by Targeting GhTT2L in Cotton (Gossypium hirsutum)

Mei,

Niu,

et al. 2023

J. Agric. Food Chem.

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Proanthocyanidins (PAs) are predominantly regulated at the transcriptional level by sophisticated regulatory networks. In cotton, the role of miRNAs as key regulatory factors at the post-transcriptional level is still unclear. Here, we demonstrated that GhmiR858 negatively regulates PA accumulation in cotton leaves and calli by targeting GhTT2L. Excessive expression of GhmiR858 restrained the expression of GhTT2L, resulting in a significant decrease in PA abundance. Conversely, a reduction in GhmiR858 activity upregulated GhTT2L, which increased PA accumulation. Additionally, GhTT2L was found to positively regulate PA accumulation in both cotton and Arabidopsis. Further analyses showed that GhTT2L interacted with transcription factor GhTTG1, which directly binds to the GhANR promoter, to facilitate its transcription. This study provides new information to guide future studies of the PA regulatory mechanisms affected by miRNAs as well as the breeding of novel varieties of colored cotton with rich PAs.

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Section: Discussionmentioning

confidence: 99%

Section: Ghmir858 Is Highly Expressed In a G Hirsutummentioning

confidence: 99%

Section: Ghtt2l Induces the Activities Of Ghanr Through Binding To Itsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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GhmiR858 Inhibits the Accumulation of Proanthocyanidins by Targeting GhTT2L in Cotton (Gossypium hirsutum)

Mei,

Niu,

et al. 2023

J. Agric. Food Chem.

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Biosynthetic pathway analysis combined with feature‐based molecular networking to comprehensively characterize the chemical constituents in seeds ofSterculia lychnophora

Liu,

Yang

et al. 2024

Phytochemical Analysis

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IntroductionThe seeds of Sterculia lychnophora Hance, commonly known as Pangdahai (PDH) in Chinese, have found extensive use in both culinary and traditional medicinal practices. However, a comprehensive understanding of the chemical composition of PDH has been lacking.ObjectivesThis study proposes a strategy that integrates biosynthetic pathway analysis with feature‐based molecular networking (FBMN), aiming for a thorough and global characterization of the chemical compositions of PDH.MethodologyThe FBMN map reveals potential compounds with structural similarity, and the MS/MS fragments could be annotated based on library matches, which could predict the plausible biosynthetic pathways in PDH, accomplishing the annotation of compounds clustered in FBMN by integrating biosynthetic pathways.ResultsConsequently, 126 compounds were plausibly or unambiguously identified, including 37 phenolic acids and glycosides, 20 flavonoids and glycosides, 12 procyanidins, 21 alkaloids, 22 lipids, and 14 others. Leveraging the information, 40 compounds, including 1 unique isoquinoline alkaloid and 2 rare linear furocoumarins, were isolated and confirmed.ConclusionsThis study not only demonstrates a highly effective approach for identifying compounds within complex herbal mixtures but also establishes a robust foundation for the further development of PDH.

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The Lotus corniculatus MYB5 functions as a master regulator in proanthocyanidin biosynthesis and bioengineering

Jiang,

Li,

Xia

et al. 2024

Plant Cell Rep

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The complexities of proanthocyanidin biosynthesis and its regulation in plants

Cited by 47 publications

References 176 publications

GhmiR858 Inhibits the Accumulation of Proanthocyanidins by Targeting GhTT2L in Cotton (Gossypium hirsutum)

GhmiR858 Inhibits the Accumulation of Proanthocyanidins by Targeting GhTT2L in Cotton (Gossypium hirsutum)

Biosynthetic pathway analysis combined with feature‐based molecular networking to comprehensively characterize the chemical constituents in seeds ofSterculia lychnophora

The Lotus corniculatus MYB5 functions as a master regulator in proanthocyanidin biosynthesis and bioengineering

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