Shenzhen, a bustling metropolis in China, is currently grappling with the delicate balance between urban expansion and environmental preservation. This study finds that the disintegration of habitats due to urban development in Shenzhen city significantly impacts the ecosystem of animals and plants, detrimentally altering their living conditions. The study results reveal that the expansion of construction land in China has surged by an impressive 84.39% between 2000 and 2020, which has come at the cost of diminishing wetlands, farmland, and woodlands by 57.24%, 16.58%, and 1.86%, respectively. Between 1980–2020, the urban expanse of Shenzhen expanded dramatically, growing from 16.0 square kilometres to 850.2 square kilometres. This transformation was primarily driven by the rapid conversion of forested areas, which saw a staggering decrease of approximately 300% to 780 km2 by 2020. Additionally, half of Shenzhen’s expansion can be attributed to the transformation of agricultural land, with an additional 40% stemming from the clearing of forests and approximately 9% resulting from the decrease in water resources. Furthermore, non-urban green spaces, particularly built-up areas, have significantly expanded, now encompassing approximately 60% of the city in 2018. Therefore, based on the existing literature and authoritative legal texts, this study aims to examine the legal frameworks of the Shenzhen government and, along with China’s national legislation, to make room for wildlife and biodiversity conservation in a metropolis like Shenzhen.