The conservation of a core virome in<i>Aedes</i>mosquitoes across different developmental stages and continents

Shi, Chengying; Zhao, Lu; Zeng, Weihua; Atoni, Evans; Hu, Xiaoming; Matthijnssens, Jelle; Yuan, Zhiming; Xia, Han

doi:10.1101/2020.04.23.058701

Cited by 11 publications

(11 citation statements)

References 64 publications

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“…Although viruses belonging to Sobemovirus genus and Luteoviridae family are known plant viruses and are of monopartite genome, Sobemo-like virus members have bi-segmented genomes and have been isolated primarily from insects [1,26]. It has been speculated that this group of viruses should be proposed as a new family [32].…”

Section: Discussionmentioning

confidence: 99%

Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species

Ribeiro

Morais

Monteiro

et al. 2020

Viruses

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Viruses are the most diverse and abundant microorganisms on earth, highly adaptive to a wide range of hosts. Viral diversity within invertebrate hosts has gained notoriety in recent years in public health as several such viruses have been of medical importance. Aedes aegypti serves as a vector for several viruses that have caused epidemics within the last year throughout Brazil; including Dengue, Zika and Chikungunya. This study aimed to identify new viral agents within Aedes aegypti mosquito in a city of the Amazonian region, where it is highly endemic. Metagenomic investigation was performed on 60 mosquito pools and viral RNA sequences present in their microbiota were characterized using genomic and phylogenetic tools. In total, we identified five putative novel virus species related to the Sobemovirus genus, Iflavirus genus and Permutatetraviridae family. These findings indicate a diverse taxonomy of viruses present in the mosquito microbiota of the Amazon, the region with the greatest invertebrate diversity in the world.

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Section: Discussionmentioning

confidence: 99%

Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species

Ribeiro

Morais

Monteiro

et al. 2020

Viruses

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“…The number of newly discovered arthropod viruses has been exponentially increasing after the development of massive parallel sequencing technologies, but in the majority of the cases, little is known about their biology and ecology as well as their impact on human health and economy [ 24 , 25 ]. It was recently shown that insects host stable populations of viruses, or core viromes, that are vertically transmitted both in laboratory colonies and in nature [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus

et al. 2021

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Triatomine assassin bugs comprise hematophagous insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Although the microbiome of these species has been investigated to some extent, only one virus infecting Triatoma infestans has been identified to date. Here, we describe for the first time seven (+) single-strand RNA viruses (RpV1-7) infecting Rhodnius prolixus, a primary vector of Chagas disease in Central and South America. We show that the RpVs belong to the Iflaviridae, Permutotetraviridae and Solemoviridae and are vertically transmitted from the mothers to the progeny via transovarial transmission. Consistent with this, all the RpVs, except RpV2 that is related to the entomopathogenic Slow bee paralysis virus, established persistent infections in our R. prolixus colony. Furthermore, we show that R. prolixus ovaries express 22-nucleotide viral siRNAs (vsiRNAs), but not viral piRNAs, that originate from the processing of dsRNA intermediates during viral replication of the RpVs. Interestingly, the permutotetraviruses and sobemoviruses display shared pools of vsiRNAs that might provide the basis for a cross-immunity system. The vsiRNAs are maternally deposited in the eggs, where they likely contribute to reduce the viral load and protect the developing embryos. Our results unveil for the first time a complex core virome in R. prolixus and begin to shed light on the RNAi-based antiviral defenses in triatomines.

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“…The term "core virome" was recently coined to describe the set of ISVs common to the majority of individuals in a mosquito population (Shi et al 2019). The term has since been divided into two categories: vertical (passed from mother to offspring) and environmental (acquired from the environment) (Shi et al 2020). To date, research on Aedes mosquitoes suggests that at the population level core viromes are maintained across developmental stages (Shi et al 2020) and over short time scales (~ 1 year) (Boyles et al 2020, Shi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The term has since been divided into two categories: vertical (passed from mother to offspring) and environmental (acquired from the environment) (Shi et al 2020). To date, research on Aedes mosquitoes suggests that at the population level core viromes are maintained across developmental stages (Shi et al 2020) and over short time scales (~ 1 year) (Boyles et al 2020, Shi et al 2020). However, it is unknown how well vertical core viromes are sustained over longer periods of time, or the degree to which environmental core viromes are transient.…”

Section: Introductionmentioning

confidence: 99%

Intrinsic variation in the vertically transmitted insect-specific core virome of Aedes aegypti

Coatsworth

Bozic

Carrillo³

et al. 2021

Preprint

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Since 2009, local outbreaks of dengue (serotypes 1-3) mediated by Aedes aegypti mosquitoes have occurred in the United States, particularly in Florida (FL). In 2016 and 2017, dengue virus serotype 4 was found alongside several insect-specific viruses (ISVs) in pools of Ae. aegypti from Manatee County, FL, in the absence of an index case. Although ISVs have been characterized in Ae. aegypti globally, the constitution of a core virome in natural populations remains unclear. Using mosquitoes sampled from Manatee County in 2018, we compared baseline ovary viromes of field (G0) and lab (Orlando) Ae. aegypti via metagenomic RNA sequencing. Across all samples, virome composition varied by sample type (field- or colony-derived). Four ISVs comprised >97% of virus sequences: a novel partiti-like virus (Partitiviridae), a previously described toti-like virus (Totiviridae), unclassified Riboviria, and four previously described orthomyxo-like viruses (Orthormyxoviridae). Whole or partial genomes for the toti-like virus, partiti-like virus, and one orthomyxo-like virus were assembled and analyzed phylogenetically. Multigenerational maintenance of these ISVs was confirmed orthogonally by RT-PCR in G0 and G7 mosquitoes, indicating vertical transmission as the mechanism for ISV sustentation. This study provides fundamental information regarding ISV ecology, persistence, and variation in Ae. aegypti in nature.

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The conservation of a core virome inAedesmosquitoes across different developmental stages and continents

Abstract: 32

Cited by 11 publications

References 64 publications

Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species

Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species

Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus

Intrinsic variation in the vertically transmitted insect-specific core virome of Aedes aegypti

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