The conserved ciliary protein Bug22 controls planar beating of<i>Chlamydomonas</i>flagella

Meng, Dan; Cao, Muqing; Oda, Toshiyuki; Pan, Junmin

doi:10.1242/jcs.140723

Cited by 33 publications

(47 citation statements)

References 35 publications

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“…Such observations may reflect the polarized nature of the flagellar axoneme. For example, the beak-like structures within doublet microtubules are found only in the proximal part of flagella (Hoops and Witman, 1983), as are the polyglutamylated tubulins (Kann et al, 2003) and the axonemal protein Bug22 (Meng et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Cilia Disassembly with Two Distinct Phases of Regulation

Liang

et al. 2015

Cell Reports

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Cilia and flagella are dynamic organelles that undergo assembly and disassembly during each cell cycle. They are structurally polarized, and the mechanisms by which these organelles are disassembled are incompletely understood. Here, we show that flagellar resorption occurs in two distinct phases of length-dependent regulation. A CDK-like kinase, encoded by flagellar shortening 1 (FLS1), is required for the normal rate of disassembly of only the distal part of the flagellum. Mechanistically, loss of function of FLS1 prevents the initial phosphorylation of CALK, an aurora-like kinase that regulates flagellar shortening, and induces the earlier onset of the inhibitory phosphorylation of CrKinesin13, a microtubule depolymerase, which is involved in flagellar shortening. In addition, CALK and CrKinesin13 phosphorylation can also be induced by the process of flagellar shortening itself, demonstrating an example of cilia-generated signaling not requiring the binding of a ligand or the stimulation of an ion channel.

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Section: Discussionmentioning

confidence: 99%

Cilia Disassembly with Two Distinct Phases of Regulation

Liang

et al. 2015

Cell Reports

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“…The disrupted genes were identified by cloning the flanking genomic sequences using RESDA PCR followed by sequencing [54, 55]. The DNA fragment with about 250 nt being deleted from 5’ end has replaced the nucleotides 215–221 of the IFT43 gene.…”

Section: Methodsmentioning

confidence: 99%

“…For palmelloid cells, the cells were treated with gametic autolysin to release the daughter cells followed by DIC microscopy. A previous procedure was used to examine the ultrastructure of the flagella by thin section EM [55, 59]. …”

Section: Methodsmentioning

confidence: 99%

Functional exploration of the IFT-A complex in intraflagellar transport and ciliogenesis

Zhu

Wang

et al. 2017

PLoS Genet

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Intraflagellar transport (IFT) particles or trains are composed of IFT-A and IFT-B complexes. To assess the working mechanism of the IFT-A complex in IFT and ciliogenesis, we have analyzed ift43 mutants of Chlamydomnonas in conjunction with mutants of the other IFT-A subunits. An ift43 null mutant or a mutant with a partial deletion of the IFT43 conserved domain has no or short flagella. The mutants accumulate not only IFT-B but also IFT-Ain the short flagella, which is in contrast to an ift140 null mutant. The IFT43 conserved domain is necessary and sufficient for the function of IFT43. IFT43 directly interacts with IFT121 and loss of IFT43 results in instability of IFT-A. A construct with a partial deletion of the IFT43 conserved domain is sufficient to rescue the instability phenotype of IFT-A, but results in diminishing of IFT-A at the peri-basal body region. We have further provided evidence for the direct interactions within the IFT-A complex and shown that the integrity of IFT-A is important for its stability and cellular localization. Finally, we show that both IFT43 and IFT140 are involved in mobilizing ciliary precursors from the cytoplasmic pool during flagellar regeneration, suggesting a novel role of IFT-A in transporting ciliary components in the cytoplasm to the peri-basal body region.

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“…The flanking sequence of the DNA insert was identified by RESDA PCR and sequencing (González-Ballester et al, 2005;Meng et al, 2014). To make a construct for rescuing kcn11, the KCN11 gene with an ∼2 kb fragment upstream of the start codon was cloned by PCR using Phanta Max Super-Fidelity DNA Polymerase (Vazyme Biotech Co. Ltd, Nanjing, China) from a bac clone BAC31O7 (Clemson University, USA) and a 3×HA tag sequence followed by a LC8 terminator was cloned from plasmid pKL-3xHA (kindly provided by Karl F. Lechtreck, University of Georgia, Athens, GA) (Lechtreck et al, 2009).…”

Section: Insertional Mutagenesis Gene Cloning and Other Molecular Tmentioning

confidence: 99%

“…To analyze the function of the new proteins, C. reinhardtii insertional mutants were generated by transforming with a DNA fragment harboring the paromomycin-resistance gene AphVIII (Meng et al, 2014;Sizova et al, 2001). We found that KCN11 was disrupted in one of the mutants by the insertion of this DNA fragment in the sixth exon of the gene (Fig.…”

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confidence: 99%

An organelle K+ channel is required for osmoregulation in Chlamydomonas reinhardtii

Jiang

et al. 2016

Journal of Cell Science

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Fresh water protozoa and algae face hypotonic challenges in their living environment. Many of them employ a contractile vacuole system to uptake excessive water from the cytoplasm and expel it to the environment to achieve cellular homeostasis. K + , a major osmolyte in contractile vacuole, is predicted to create higher osmolarity for water influx. Molecular mechanisms for K + permeation through the plasma membrane have been well studied. However, how K + permeates organelles such as the contractile vacuole is not clear. Here, we show that the six-transmembrane K + channel KCN11 in Chlamydomonas is exclusively localized to contractile vacuole. Ectopic expression of KCN11 in HEK293T cells results in voltage-gated K + channel activity. Disruption of the gene or mutation of key residues for K + permeability of the channel leads to dysfunction of cell osmoregulation in very hypotonic conditions. The contractile cycle is inhibited in the mutant cells with a slower rate of contractile vacuole swelling, leading to cell death. These data demonstrate a new role for six-transmembrane K + channels in contractile vacuole functioning and provide further insights into osmoregulation mediated by the contractile vacuole.

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The conserved ciliary protein Bug22 controls planar beating ofChlamydomonasflagella

Cited by 33 publications

References 35 publications

Cilia Disassembly with Two Distinct Phases of Regulation

Cilia Disassembly with Two Distinct Phases of Regulation

Functional exploration of the IFT-A complex in intraflagellar transport and ciliogenesis

An organelle K+ channel is required for osmoregulation in Chlamydomonas reinhardtii

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