The Construction and Comprehensive Analysis of Inflammation-Related ceRNA Networks and Tissue-Infiltrating Immune Cells in Ulcerative Colitis Progression

Abstract

Ulcerative colitis (UC) is a common disease with great variability in severity, with a high recurrence rate and heavy disease burden. In recent years, the different biological functions of competing endogenous RNA (ceRNA) networks of long noncoding RNAs (lncRNAs) and microRNAs (miRs) have aroused wide concerns, the ceRNA network of ulcerative colitis (UC) may have potential research value, and these expressed noncoding RNAs may be involved in the molecular basis of inflammation recurrence and progression. This study analyzed 490 colon samples associated with UC from 4 gene expression microarrays from the GEO database and identified gene modules by weighted correlation network analysis (WGCNA). CIBERSORT detected tissue-infiltrating leukocyte profiling by deconvolution of microarray data. LncBase and multiMIR were used to identify lncRNA-miRNA-mRNA interaction. We constructed a ceRNA network which includes 4 lncRNAs (SH3BP5-AS1, MIR4435-2HG, ENTPD1-AS1, and AC007750.1), 5 miRNAs (miR-141-3p, miR-191-5p, miR-192-5p, miR-194-5p, and miR196-5p), and 52 mRNAs. Those genes are involved in interleukin family signals, neutrophil degranulation, adaptive immunity, and cell adhesion pathways. lncRNA MIR4435-2HG is a variable in the decision tree for moderate-to-severe UC diagnostic prediction. Our work identifies potential regulated inflammation-related lncRNA-miRNA-mRNA regulatory axes. The regulatory axes are dysregulated during the deterioration of UC, suggesting that it is a risk factor for UC progression.

Identification of pyroptosis-related lncRNAs as potential prognostic biomarkers in patients with adenocarcinoma of the esophagogastric junction

“…ENTPD1-AS1, located on chromosome 10q24.1, and also known as RP11-429G19.2, was involved in the immune-related pathways of in ammation recurrence in ulcerative colitis [35], and was demonstrated to be a predictor of prognosis of glioblastoma multiforme [36]. Our study results revealed that the three pyroptosis-related lncRNAs used to assess the risk model were independent prognostic factors of clinical stage and could robustly predict the prognosis of AEG.…”

N6-methyladenosine-mediated SH3BP5-AS1 upregulation promotes GEM chemoresistance in pancreatic cancer by activating the Wnt signaling pathway

“…SH3BP5-AS1 is closely related to PC drug resistance. Only two studies have reported that SH3BP5-AS1 might be related to in ammation and the prognosis of head and neck tumors [9,23] ; however, the role of SH3BP5-AS1 has not been studied in PC. It is important to fully elucidate the mechanism of SH3BP5-AS1 in the drug resistance of PC.…”

Knockdown of long non‑coding RNA NEAT1 relieves inflammation of ulcerative colitis by regulating the miR‑603/FGF9 pathway

“…Despite recent efforts, the treatment and diagnosis of IBD remains a challenge in clinical studies ( 27 , 28 ). Thus, recent studies have aimed to investigate the underlying molecular mechanisms of IBD development and disease progression ( 29-31 ). lncRNAs are critical diagnostic and therapeutic biomarkers, which act as competitive endogenous RNAs ( 32 ).…”