The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by Lactobacillus plantarum NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens

Abstract

Interleukin-17B (IL-17B) is a protective cytokine of the IL-17 family and plays an essential role in the regulation of mucosal inflammation. However, little is known about the role of IL-17B in the control of viral infections. In this study, a recombinant Lactobacillus plantarum, designated as NC8-ChIL17B, was constructed to express the chicken IL-17B (ChIL-17B) gene. The recombinant ChIL17B (rChIL17B) protein was about 14 kDa and was anchored to the surface of NC8 cells. In vitro, it was found that the rChIL17B protein inhibited the proliferation of the infectious bronchitis virus (IBV) through activation of nuclear factor kappa B (NF-κB) and the JAK (Janus kinase)-STAT (signal transducers and activators of transcription) signaling. Moreover, to evaluate the immunoadjuvant activities of NC8-ChIL17B, 40 three-day-old specific pathogen-free (SPF) chickens were divided into four groups. Three groups were orally vaccinated with fresh NC8, NC8-ChIL17B, and phosphate buffered saline (PBS), along with the infectious bronchitis virus vaccine, and the other group was the PBS-negative control. The results of the IBV-specific antibody titer and the concentration of the cytokines IL-2, IL-4, IL-6, and interferon gamma (IFN-γ) in sera, as well as the concentration of secretory immunoglobulin A (sIgA) in the tracheal and small intestinal mucosa, the number of cluster of differentiation 4 positive (CD4+) and cluster of differentiation 8 positive (CD8+) T cells in the blood, and the expression of immune-related genes all indicated that NC8-ChIL17B efficiently enhanced the humoral and cellular immune responses to IBV vaccine. Moreover, the viral loads in the NC8-ChIL17B- and IBV-vaccinated group were significantly lower than in the control groups, suggesting a significant promotion of the immunoprotection of IBV vaccination against the virulent IBV strain. Therefore, ChIL-17B is a promising, effective adjuvant candidate for chicken virus vaccines.

Porcine Interleukin-17 and 22 Co-Expressed by Yarrowia lipolytica Enhance Immunity and Increase Protection against Bacterial Challenge in Mice and Piglets

“…In our animal experiments, the mice in the Po1h-pINA1297-IL-17/22 group displayed an evident promotion of growth compared to the control groups. Our previous works also found similar results that fusion protein IL-4/6 engineered by the same technique or single IL-23 and IL-17B both improved the animals’ growth performance [ 28 , 29 , 30 ]. The results indicate that Po1h-pINA1297-IL-17/22 has the effect of increasing body weight gain.…”

Immune-adjuvant effect of vitamin A and probiotics supplementation on humoral response to cell culture rabies vaccine in rabbits

“…As stated in many studies, probiotics can enhance innate and adaptive immunity. This has been demonstrated in a study on fish with probiotics supplementation, showing that probiotic administration through the oral route can influence both the systemic and the local immunity of the host animals (Guo et al 2020;Simon et al 2021).…”

Rapid and Highly Efficient Genetic Transformation and Application of Interleukin-17B Expressed in Duckweed as Mucosal Vaccine Adjuvant

“…Approximately 1.0 × 10 6 cells seeded in a six-well plate were stimulated with recombination IL-17B protein (200 ng/mL) for 4 h. Total RNA was extracted from DF-1 cells using Trizol reagent and then reversely transcribed into cDNA by applying a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Japan). The primers [ 36 ] and AceQR qPCR SYBR Green Master Mix (Vazyme Biotech Co., Ltd., Nangjing, China) were used to conduct real-time PCR with Bio-Rad CFX connect (Bio-Rad, Berkeley, CA, USA). All qPCR assays followed the program: 5 mins at 95 °C, followed by 40 cycles of 10 s at 95 °C, and 30 s at 60 °C.…”

“…Interleukin-17B (IL-17B), a member of the IL-17 family, plays a key role in regulating the expression of proinflammatory cytokines. Chicken chIL-17B produced from Lactobacillus plantarum NC8 has been reported to enhance chicken’s immune responses to the oral IBV attenuated vaccine, exhibiting potential adjuvanticity on mucosal vaccination [ 36 ].…”