The Content of Cytokines IL-6, IL-8, TNF-α, IL-4 and the Level of CD86 and CD163 Expression in Peritoneal Fluid Macrophages Has a Reverse Correlation with the Degree of Severity of External Genital Endometriosis

Krasnyi, Krasnyi A.M.; Sadekova, Sadekova A.A.; Sefihanov, T G; Вторушина, В В; Кречетова, Л. В.; Khilkevich, E. G.; Arakelyan, A.S.; Павлович, С. В.

doi:10.1134/s1990750820010096

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…This may be crucial for the development of endometriosis, since it is believed that the PF macrophages eliminate the endometrial cells that are brought to the abdominal cavity by retrograde menstruation. Earlier, we studied pro- and anti-inflammatory activities of macrophages in the PF of endometriosis patients and came to the conclusion that in severe endometriosis both activities did not differ from those in the control group but were increased in patients with mild endometriosis [ 13 ]. These findings indicated that macrophages may not “recognize” severe endometriosis, possibly being under modulating influence of unknown factors.…”

Section: Discussionmentioning

confidence: 99%

“…Our studies of peritoneal macrophages showed that even though the ratio of pro-inflammatory/anti-inflammatory markers decreased with increasing severity of endometriosis, there was no trend of polarization of macrophages to M1 or M2 states. There was a direct correlation between pro-and anti-inflammatory surface markers and cytokines in PF [ 13 ]. This finding indicates that the anti-inflammatory activity of peritoneal macrophages is reduced in endometriosis.…”

Section: Introductionmentioning

confidence: 99%

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The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages

Krasnyi

Sadekova

Smolnova

et al. 2022

IJMS

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The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophages in the peritoneal fluid (PF) in patients with endometriosis. The study included 54 women with endometriosis and a control group of 30 women with uterine myoma without signs of endometriosis. The levels of factors in PF were assessed by a multiplex method. Expression of GLP1R receptors, CD10, CD26 proteases, and CD86 by macrophages was evaluated using flow cytometry. It was found that in women with endometriosis, the concentrations of ghrelin, GLP-1, glucagon, and visfatin in PF were reduced (p = 0.007, p = 0.009, p = 0.002, p = 0.008, respectively). At the same time, there was a noted increase in the CD10 protease expression by peritoneal macrophages (p = 0.044). Correlation analysis showed a positive correlation of ghrelin and GLP-1 levels with CD86 macrophage expression (p = 0.044, p = 0.022, respectively) in the study group; a positive correlation was also found between the levels of GLP-1, glucagon, and visfatin with CD26 macrophage expression (p = 0.041, p = 0.048, p = 0.015, respectively) in PF. No correlations were found in the control group. These results indicate that a decrease in the levels of ghrelin, GLP-1, glucagon, and visfatin in PF may contribute to endometriosis development through their impact on the expression of pro-inflammatory markers of PF macrophages.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages

Krasnyi

Sadekova

Smolnova

et al. 2022

IJMS

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“…Дисбаланс цитокиновой активности может способствовать развитию хронического воспаления, такого как эндометриоз [3,5,6,7,8,9,11,13,15,16,17].…”

Section: Discussionunclassified

“…Krasnyi A.M. и соавт., которые исследовали уровни цитокинов (IL-6, IL-8, IL-10, TNF и др.) в сыворотке крови женщин с эндометриозом, сообщают, что развитие эндометриоза может быть обусловлено недостаточностью как противовоспалительной, так и провоспалительной активностью макрофагов [13].…”

Section: Discussionunclassified

Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene

Kazakova

Dolgikh

2021

Med. immunol.

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Excessive activity of cytokines, as well as their deficiency, promote a regulatory imbalance of immune system, which may cause persistent inflammation and, as a consequence, loss of pregnancy. The purpose of our work was to study the role of cytokine expression in the development of endometriosis in women exposed to excessive environmental contamination with hydroxybenzene. The study involved 106 women of reproductive age, divided into 4 groups according to two criteria: the presence or absence of reproductive disorders (endometriosis), as well as levels of blood contamination with hydroxybenzene compared to the reference ranges. The groups of women were comparable in age, ethnicity, and social status. The level of phenol in blood was measured by gas chromatography, and the cytokine levels were determined by enzyme immunoassay. The use of parametric and nonparametric statistical criteria as well as adjustments for multiple Bonferoni comparisons, allowed us to establish significant differences between the groups, according to the levels of IL-1β, IL-8, IL-10. The expression of interleukin 8 (IL-8) in women with endometriosis was found to be significantly higher than in healthy ones. At the same time, the observed features of IL-8, interleukin 1β (IL-1β) expression correlate with the overproduction of interleukin 10 (IL-10) associated with excessive contamination of bio-environments with hydroxybenzene. The results of using non-parametric correlation analysis of the Spearman data revealed a positive correlation between IL-10 production and hydroxybenzene levels in the blood, as well as inverse relationship between IL-10 and IL-8 expression. Thus, evidence has been obtained of the involvement of exogenous estrogen hydroxybenzene in development of reproductive disorders, probably, formed under active participation of increased anti-inflammatory IL-10 cytokine, which, by antagonism to proinflammatory mediators (e.g., IL-8), seems to promote apparent transition from acute phase of endometriosis to chronic disorder, thus reducing the reproductive potential of women. It is known that IL-8 expression significantly correlates with development of endometriosis, but excessive phenol exposure may increase the anti-inflammatory IL-10 expression, thereby suppressing the activity of IL-8. Suppressed activity of proinflammatory cytokines by phenol may lead to chronic inflammation and impaired reproductive functions.

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Synergy between Th1 and Th2 responses during endometriosis: A review of current understanding

L.A.

Shan

Hou

et al. 2023

Journal of Reproductive Immunology

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The Content of Cytokines IL-6, IL-8, TNF-α, IL-4 and the Level of CD86 and CD163 Expression in Peritoneal Fluid Macrophages Has a Reverse Correlation with the Degree of Severity of External Genital Endometriosis

Cited by 4 publications

References 19 publications

The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages

The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages

Cytokine profile features in women with reproductive disorders exposed to excessive environmental contamination with hydroxybenzene

Synergy between Th1 and Th2 responses during endometriosis: A review of current understanding

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