2018
DOI: 10.1128/jcm.00437-18
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The Continued Value of Disk Diffusion for Assessing Antimicrobial Susceptibility in Clinical Laboratories: Report from the Clinical and Laboratory Standards Institute Methods Development and Standardization Working Group

Abstract: Expedited pathways to antimicrobial agent approval by the U.S. Food and Drug Administration (FDA) have led to increased delays between drug approval and the availability of FDA-cleared antimicrobial susceptibility testing (AST) devices. Antimicrobial disks for use with disk diffusion testing are among the first AST devices available to clinical laboratories. However, many laboratories are reluctant to implement disk diffusion testing for a variety of reasons, including dwindling proficiency with this method, i… Show more

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Cited by 65 publications
(48 citation statements)
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“…The antibiotics tested were penicillin (PCN), oxacillin (OXA), gentamicin (GEN), ciprofloxacin (CIP), levofloxacin (LEV), moxifloxacin (MXF), erythromycin (EM), clindamycin (CM), linezolid (LZD), vancomycin (VAN), tetracycline (TET), rifampicin (RIF) and trimethoprim/ sulfamethoxazole (SXT). The tests were performed and interpreted in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines (M100, 2018) [16], and S. aureus ATCC 29213 was used as a quality control. The mec gene was detected by PCR as described by previous study [17].…”
Section: Antibiotic-resistant Testmentioning
confidence: 99%
“…The antibiotics tested were penicillin (PCN), oxacillin (OXA), gentamicin (GEN), ciprofloxacin (CIP), levofloxacin (LEV), moxifloxacin (MXF), erythromycin (EM), clindamycin (CM), linezolid (LZD), vancomycin (VAN), tetracycline (TET), rifampicin (RIF) and trimethoprim/ sulfamethoxazole (SXT). The tests were performed and interpreted in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines (M100, 2018) [16], and S. aureus ATCC 29213 was used as a quality control. The mec gene was detected by PCR as described by previous study [17].…”
Section: Antibiotic-resistant Testmentioning
confidence: 99%
“…Compact system with Gram-negative bacteria cards (bioMérieux, Lyon, France). The Etest method was employed to verify the drug sensitivity of 5 antibiotics, and the results were interpreted based on the CLSI guidelines [12] [13]. Escherichia coli (ATCC 25922) was used as the quality control strain for antimicrobial susceptibility testing.…”
Section: Antimicrobial Susceptibility Testingmentioning
confidence: 99%
“…The antibiotic resistance of Hp was measured by dilution methods with reference to the protocols of the Clinical and Laboratory Standards Institute (Wayne, PA, USA) [18] . Brie y, the concentration of Hp was regulated to be 1×10 6 CFU/ml and incubated at 37 °C for 3~5 days under microaerophilic conditions.…”
Section: Antibiotic Susceptibility Testingmentioning
confidence: 99%