The contractile properties of the medial gastrocnemius motor units innervated by L4 and L5 spinal nerves in the rat

Celichowski, Jan; Taborowska, M.

doi:10.3109/08990220.2011.598692

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…This paper presents a straightforward and reproducible model of apoptosis of spinal cord VHMNs following third-degree burn injuries. Reportedly, third-degree burns on the hindpaw (dermatomes L3–L5) [ 31 , 32 ] induce apoptosis of the spinal cord VHMNs at the corresponding dermatomes and consequently cause gastrocnemius muscle wasting (innervated by L4 and L5) [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats

Huang

Cheng

et al. 2015

BioMed Research International

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Background. Severe burns result in hypercatabolic state and concomitant muscle atrophy that persists for several months, thereby limiting patient recovery. However, the effects of burns on the corresponding spinal dermatome remain unknown. This study aimed to investigate whether burns induce apoptosis of spinal cord ventral horn motor neurons (VHMNs) and consequently cause skeletal muscle wasting. Methods. Third-degree hindpaw burn injury with 1% total body surface area (TBSA) rats were euthanized 4 and 8 weeks after burn injury. The apoptosis profiles in the ventral horns of the lumbar spinal cords, sciatic nerves, and gastrocnemius muscles were examined. The Schwann cells in the sciatic nerve were marked with S100. The gastrocnemius muscles were harvested to measure the denervation atrophy. Result. The VHMNs apoptosis in the spinal cord was observed after inducing third-degree burns in the hindpaw. The S100 and TUNEL double-positive cells in the sciatic nerve increased significantly after the burn injury. Gastrocnemius muscle apoptosis and denervation atrophy area increased significantly after the burn injury. Conclusion. Local hindpaw burn induces apoptosis in VHMNs and Schwann cells in sciatic nerve, which causes corresponding gastrocnemius muscle denervation atrophy. Our results provided an animal model to evaluate burn-induced muscle wasting, and elucidate the underlying mechanisms.

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Section: Discussionmentioning

confidence: 99%

Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats

Huang

Cheng

et al. 2015

BioMed Research International

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Autologous adipose-derived stem cells attenuate muscular atrophy and protect spinal cord ventral horn motor neurons in an animal model of burn injury

Huang

et al. 2015

Cytotherapy

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Morphometric properties and innervation of muscle compartments in rat medial gastrocnemius

Taborowska¹,

Bukowska²,

Drzymała‐Celichowska³

et al. 2016

Somatosensory & Motor Research

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The rat medial gastrocnemius (MG) muscle is composed of the proximal and distal compartments. In this study, morphometric properties of the compartments and their muscle fibres at five levels of the muscle length and the innervation pattern of these compartments from lumbar segments were investigated. The size and number of muscle fibres in the compartments were different. The proximal compartment at the largest cross section (25% of the muscle length) had 34% smaller cross-sectional area but contained a slightly higher number of muscle fibres (max. 5521 vs. 5360) in comparison to data for the distal compartment which had the largest cross-sectional area at 40% of the muscle length. The muscle fibre diameters revealed a clear tendency within both compartments to increase along the muscle (from the knee to the Achilles tendon) up to 46.9 μm in the proximal compartment and 58.4 μm in the distal one. The maximal tetanic and single twitch force evoked by stimulation of L4, L5, and L6 ventral roots in whole muscle and compartments were measured. The MG was innervated from L4 and L5, only L5, or L5 and L6 segments. The proximal compartment was innervated by axons from L5 or L5 and L4, and the distal one from L5, L5 and L6, or L5 and L4 segments. The forces produced by the compartments summed non-linearly. The tetanic forces of the proximal and distal compartments amounted to 2.24 and 4.86 N, respectively, and their algebraic sums were 11% higher than the whole muscle force (6.37 N).

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The contractile properties of the medial gastrocnemius motor units innervated by L4 and L5 spinal nerves in the rat

Cited by 3 publications

References 29 publications

Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats

Third-Degree Hindpaw Burn Injury Induced Apoptosis of Lumbar Spinal Cord Ventral Horn Motor Neurons and Sciatic Nerve and Muscle Atrophy in Rats

Autologous adipose-derived stem cells attenuate muscular atrophy and protect spinal cord ventral horn motor neurons in an animal model of burn injury

Morphometric properties and innervation of muscle compartments in rat medial gastrocnemius

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