The contribution of farm animals to human health

Kues, Wilfried August

doi:10.1016/s0167-7799(04)00108-8

Cited by 117 publications

(54 citation statements)

References 35 publications

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“…Transcriptomic studies of in vivo developing embryos recovered from livestock species such as cattle are valuable for two main reasons (5,6). First, Bos taurus is an economically important species for which advanced reproduction technology has been developed (7).…”

Section: Genome-wide Expression Profiling Reveals Distinct Clusters Omentioning

confidence: 99%

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Kues

Sudheer

Herrmann³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

155

113

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Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their in vivo counterparts in many aspects and exhibit a higher proportion of developmental abnormalities. Here, we determined for the first time the transcriptomes of bovine metaphase II oocytes and all stages of preimplantation embryos developing in vivo up to the blastocyst using the Affymetrix GeneChip Bovine Genome Array which examines approximately 23,000 transcripts. The data show that bovine oocytes and embryos transcribed a significantly higher number of genes than somatic cells. Several hundred genes were transcribed well before the 8-cell stage, at which the major activation of the bovine genome expression occurs. Importantly, stage-specific expression patterns in 2-cell, 4-cell, and 8-cell stages, and in morulae and blastocysts, were detected, indicating dynamic changes in the embryonic transcriptome and in groups of transiently active genes. Pathway analysis revealed >120 biochemical pathways that are operative in early preimplantation bovine development. Significant differences were observed between the mRNA expression profiles of in vivo and in vitro matured oocytes, highlighting the need to include in vivo derived oocytes/embryos in studies evaluating assisted reproductive techniques. This study provides the first comprehensive analysis of gene expression and transcriptome dynamics of in vivo developing bovine embryos and will serve as a basis for improving assisted reproductive technology.

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Section: Genome-wide Expression Profiling Reveals Distinct Clusters Omentioning

confidence: 99%

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Kues

Sudheer

Herrmann³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

155

113

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“…Today, the most widely used methods for the production of transgenic farm animals are: (1) direct microinjection of foreign DNA into the pronuclei of fertilised eggs; (2) nuclear transfer using genetically modified embryonic or somatic donor cells; and (3) viral-based constructs as vectors for the introduction of exogenous DNA into embryos (Kues and Niemann 2004). These methods are afflicted by low efficiency, elevated management outlay and, last but not least, the manipulation of embryos at early stages of development.…”

Section: Introductionmentioning

confidence: 99%

Sperm-mediated gene transfer

Lavitrano

Busnelli

Cerrito

et al. 2006

Reprod. Fertil. Dev.

115

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Abstract. Since 1989, a new method for the production of transgenic animals has been available, namely spermmediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition, we successfully adapted and optimised the technique for use in large animals; it was, in fact, highly efficient in the generation of human decay accelerating factor transgenic pig lines, as well as multigene transgenic pigs in which three different reporter genes, namely enhanced green fluorescent protein, enhanced blue fluorescent protein and red fluorescent protein, were introduced. The major benefits of the SMGT technique were found to be its high efficiency, low cost and ease of use compared with other methods. Furthermore, SMGT does not require embryo handling or expensive equipment. Sperm-mediated gene transfer could also be used to generate multigene transgenic pigs that would be of benefit as large animal models for medical research, for agricultural and pharmaceutical applications and, in particular, for xenotransplantation, which requires extensive genetic manipulation of donor pigs to make them suitable for grafting to humans.

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“…Most cloned embryos died before birth, and a few that develop to term showed a high occurrence of newborn death and abnormalities (Renard et al 2002;Yang et al 2007). Generally, it has been reported that the survival rate of cloned pig embryos was much lower than that of other animals (Kues & Niemann 2004). In addition, cloning of gene-targeted animals by SCNT is very difficult and costly.…”

Section: Discussionmentioning

confidence: 96%

Methylation and expression changes in imprinted genesH19andIgf2during serial somatic cell nuclear transfer using piglet fibroblasts

Jang

Kim

et al. 2015

Animal Cells and Systems

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Cloned pigs produced by somatic cell nuclear transfer (SCNT) are important as a potential alternative source of organs. Although SCNT has created new possibilities for targeted gene modification, the successful cloning of pigs is rare. Here, we successfully conducted serial SCNT for three generations. We determined that the piglet genome was inherited from donor cell nuclei using microsatellite analysis of each generation. The methylation of differentially methylated regions (DMRs) in H19 was gradually reduced over the three generations of serial SCNT. By contrast, methylation of the insulin-like growth factor 2 (Igf2) DMR increased in the F1 generation, compared to the F0, and remained at the higher level in the F2 and F3 generations. The methylation patterns of housekeeping genes such as GAPDH and β-actin were unchanged in the serially cloned pigs. In addition, expression levels of H19 and Igf2 were variable for each generation of serial SCNT piglets, but there was no clear relationship between the methylation and gene expression patterns. Our study conclusively demonstrates that the methylation patterns of DMRs in H19 and Igf2 were altered, compare to the F0 donor, during serial SCNT, but housekeeping genes were unaffected.

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The contribution of farm animals to human health

Cited by 117 publications

References 35 publications

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo

Sperm-mediated gene transfer

Methylation and expression changes in imprinted genesH19andIgf2during serial somatic cell nuclear transfer using piglet fibroblasts

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