The Contribution of Interleukin (17) in Iraqi Patients with Type I Diabetic and Positive Agglutination Sera with Morganella Morganii Antigens

Abstract

Background: The rising population of patients with diabetes type 1 has caused in a fast increase in the amount of patients who have diabetic complications. Objective: Isolation of bacteria from diabetic patients and detection of their antibodies in the serum of patients and rabbits. Materials and Methods: Sixty samples of blood and urine were collected from diabetic patients, a similar proportion of genders were included in 50 samples of blood from healthy as controls. Patients with diabetes diseases diagnosed by a physician collected from specialized center for endocrinology and diabetes and Kindy hospital, in Baghdad. Results: This study showed that Morganella morganii (n=9, 18%) was isolated from urine of patients. With M. morganii antigens (complete cells) as three bacteria antigens agglutinated in titer 1:64 and three in titer 1:128 of serum of patients (antibodies) whereas 1 and fife of M. morganii antigens agglutinated in serum of patients in titer 1:256 and 1:16 Consecutively. Determination of the concentration of cytokinetic proteins IL-17, using an ELISA device. The concentration of some inflammation-initiating cytokines was estimated in the serum of the studied samples, which included using IL-17 ELISA technology. The statistical results by t-test showed were significant differences between this interleukin between the patients and the control sample and under the probability level P<0.05 in the serum of the patients compared to the control sample. The results also showed a decrease in the cytokinetic concentration, as the concentration in the infected serum was 0.011 ± 0.019 (pg/mm), while its concentration in the control sample was 0.007 ± 0.033 (pg/mm) in the infected serum. Recognize the agglutination ability slide agglutination exam (anti Morganella morganii rabbit serum). Conclusion: The ability of killed antigen bacteria isolated from diabetic patients to agglutinate with their serum, and its ability to agglutinate with the serum of rabbits injected with this bacterium. The increased prevalence of agglutination and increased antibodies of patients and rabbit blood suggest this as part of the multifactorial basis for disease penetrance and susceptibility.