The control of microbially induced corrosion by methyl eugenol – A dietary phytochemical with quorum sensing inhibitory potential

Packiavathy, Issac Abraham Sybiya Vasantha; Maruthamuthu, S.; Gnanasekaran, Gnanaselvan; Manoharan, Subbaiah; Paul, J. John; Annapoorani, Angusamy; Kannappan, Arunachalam; Ravi, Arumugam Veera

doi:10.1016/j.bioelechem.2019.04.010

Cited by 17 publications

(11 citation statements)

References 18 publications

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“…Similar to our results, a previous study showed that after several days of QSI treatment, a shift of community-dominating species from Oceanospirillales to Rhodobacterales was observed [26]. These results showed that QSI can regulate the relative abundances of bacteria and alter the biodiversity of a symbiotic community, thereby disturbing the "normal flora", and eventually leading to biofilm inhibition [27]. However, there was no significant difference between the experimental groups (A, D, and E) and the control group (C) at 180 h, suggesting that QSI exhibits biological activity during the early stage of biofilm formation, while only having limited ability at the later stage when the biofilm is reaching the maturity stage.…”

Section: Discussionsupporting

confidence: 91%

The Rhodamine Isothiocyanate Analogue as a Quorum Sensing Inhibitor Has the Potential to Control Microbially-Induced Biofouling

et al. 2020

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Quorum sensing inhibitors (QSIs) have been proven to be an innovative approach to interfering with biofilm formation, since this process is regulated by QS signals. However, most studies have focused on single-species biofilm formation, whereas studies of the effects of signal interference on the development of multispecies biofilm, especially in the natural environment, are still lacking. Here we develop and evaluate the anti-biofilm capability of a new QSI (rhodamine isothiocyanate analogue, RIA) in natural seawater. During the experiment, biofilm characteristics, microbial communities/functions and network interactions were monitored at 36, 80, and 180 h, respectively. The results showed that the biomass and 3D structure of the biofilm were significantly different in the presence of the QSI. The expression of genes involved in extracellular polysaccharide synthesis was also downregulated in the QSI-treated group. Dramatic differences in microbial composition, β-diversity and functions between the RIA-treated group and the control group were also observed, especially in the early stage of biofilm development. Furthermore, co-occurrence model analysis showed that RIA reduced the complexity of the microbial network. This study demonstrates that rhodamine isothiocyanate analogue is an efficient QS inhibitor and has potential applications in controlling biofouling caused by multispecies biofilm, especially in the early stage of biofouling formation.

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Section: Discussionsupporting

confidence: 91%

The Rhodamine Isothiocyanate Analogue as a Quorum Sensing Inhibitor Has the Potential to Control Microbially-Induced Biofouling

et al. 2020

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“…aeruginosa) and Figure 3G,H (E. coli). 55 This is an important finding as it proves that the peptide's function is retained after the covalent attachment into a conjugate.…”

Section: Resultsmentioning

confidence: 85%

“…Consistently, SEM images were recorded, and a prevention of the bacteria biofilm is observed. This suggests that the conjugates do not permit the quorum sensing of bacteria in the biofilm process; see Figure D,E ( P. aeruginosa ) and Figure G,H ( E. coli ) . This is an important finding as it proves that the peptide’s function is retained after the covalent attachment into a conjugate.…”

Section: Resultsmentioning

confidence: 91%

Antimicrobial Polymer–Peptide Conjugates Based on Maximin H5 and PEG to Prevent Biofouling of E. coli and P. aeruginosa

Ortiz-Gómez

Rodríguez-Ramos

Maldonado‐Hernández

et al. 2020

ACS Appl. Mater. Interfaces

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Many pathogens, such as Pseudomonas aeruginosa and Escherichia coli bacteria can easily attach to surfaces and form stable biofilms. The formation of such biofilms in surfaces presents a problem in environmental, biomedical, and industrial processes, among many others. Aiming to provide a plausible solution to this issue, the anionic and hydrophobic peptide Maximin H5 C-terminally deaminated isoform (MH5C) has been modified with a cysteine in the C-terminal (MH5C-Cys) and coupled to polyethylene glycol (PEG) polymers of varying sizes (i.e., 2 kDa and 5 kDa) to serve as a surface protective coating. Briefly, the MH5C-Cys was bioconjugated to PEG and purified by size exclusion chromatography while the reaction was confirmed via SDS-PAGE and MALDI ToF. Moreover, the preventive antimicrobial activity of the MH5C-Cys-PEG conjugates was performed via the growth curves method, showing inhibition of bacterial growth after 24 h. The efficacy of these peptide−polymer conjugates was extensively characterized via scanning electron microscopy (SEM), minimum inhibition concentration (MIC), minimum biofilm inhibition concentration (MBIC), and minimum biofilm eradication concentration (MBEC) assays to evaluate their ability to eradicate and prevent the biofilms. Interestingly, this work demonstrated a critical PEG polymer weight of 5 kDa as ideal when coupled to the peptide to achieve inhibition and eradication of the biofilm formation in both bacteria strains. According to the MICs (40 μM) and MBICs (300 μM), we can conclude that this conjugate (MH5C-Cys-5 kDa) has an action that prevents/inhibits the formation of biofilms and the eradication of biofilms (MBEC 500 μM). In contrast, the MH5C-Cys peptide with PEG polymer of 2 kDa did not show inhibition or eradication of the biofilms.

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“…Similarly, beta-diversity alteration was found according to the NMDS results ( Figure 4 d), indicating the potential separation of the treatment group (PL) from the control and blank groups (P, L and N) as early as the first week. These results suggest that TRP can change the biodiversity of a symbiotic community, alter the relative richness of bacteria, and disturb the normal flora, thereby suppressing biofilm formation [ 30 ]. Notably, the microbial communities of biofilms under the four different conditions were clearly separated in the initial stage (first week), whereas some overlap appeared between treatment and control groups in the final stage (fourth week) ( Figure S3 ).…”

Section: Discussionmentioning

confidence: 99%

The Microbial Mechanisms of a Novel Photosensitive Material (Treated Rape Pollen) in Anti-Biofilm Process under Marine Environment

Wang

Zeng

et al. 2022

IJMS

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Marine biofouling is a worldwide problem in coastal areas and affects the maritime industry primarily by attachment of fouling organisms to solid immersed surfaces. Biofilm formation by microbes is the main cause of biofouling. Currently, application of antibacterial materials is an important strategy for preventing bacterial colonization and biofilm formation. A natural three-dimensional carbon skeleton material, TRP (treated rape pollen), attracted our attention owing to its visible-light-driven photocatalytic disinfection property. Based on this, we hypothesized that TRP, which is eco-friendly, would show antifouling performance and could be used for marine antifouling. We then assessed its physiochemical characteristics, oxidant potential, and antifouling ability. The results showed that TRP had excellent photosensitivity and oxidant ability, as well as strong anti-bacterial colonization capability under light-driven conditions. Confocal laser scanning microscopy showed that TRP could disperse pre-established biofilms on stainless steel surfaces in natural seawater. The biodiversity and taxonomic composition of biofilms were significantly altered by TRP (p < 0.05). Moreover, metagenomics analysis showed that functional classes involved in the antioxidant system, environmental stress, glucose–lipid metabolism, and membrane-associated functions were changed after TRP exposure. Co-occurrence model analysis further revealed that TRP markedly increased the complexity of the biofilm microbial network under light irradiation. Taken together, these results demonstrate that TRP with light irradiation can inhibit bacterial colonization and prevent initial biofilm formation. Thus, TRP is a potential nature-based green material for marine antifouling.

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The control of microbially induced corrosion by methyl eugenol – A dietary phytochemical with quorum sensing inhibitory potential

Cited by 17 publications

References 18 publications

The Rhodamine Isothiocyanate Analogue as a Quorum Sensing Inhibitor Has the Potential to Control Microbially-Induced Biofouling

The Rhodamine Isothiocyanate Analogue as a Quorum Sensing Inhibitor Has the Potential to Control Microbially-Induced Biofouling

Antimicrobial Polymer–Peptide Conjugates Based on Maximin H5 and PEG to Prevent Biofouling of E. coli and P. aeruginosa

The Microbial Mechanisms of a Novel Photosensitive Material (Treated Rape Pollen) in Anti-Biofilm Process under Marine Environment

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