The controlling sequence for site-specific chromosome breakage in tetrahymena

Yao, Meng-Chao; Yao, Ching-Ho; Monks, Bob

doi:10.1016/0092-8674(90)90142-2

Cited by 125 publications

(103 citation statements)

References 28 publications

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“…First, we could not find any correlation between MAC chromosome size and G+C content in the T. thermophila somatic genome (R 2 = 0.027), using the 125 scaffolds from the draft genome assembly that are capped by telomeres at both ends (Supplemental Table S3 from Eisen et al 2006). This is consistent with our model, since MAC chromosome fragmentation results from site-specific cleavage of the germline DNA in Tetrahymena (Yao et al 1990) and there does not appear to be any correlation between the sizes of the ∼225 MAC chromosomes and the size of the five MIC chromosomes from which they derive, judging from available assignment of telomere-capped MAC scaffolds to MIC linkage groups (Supplemental Fig. 4 from Eisen et al 2006).…”

Section: Germline Chromosomessupporting

confidence: 78%

Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: A somatic view of the germline

Duret¹,

Cohen²,

Jubin³

et al. 2008

Genome Res.

112

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Ciliates are the only unicellular eukaryotes known to separate germinal and somatic functions. Diploid but silent micronuclei transmit the genetic information to the next sexual generation. Polyploid macronuclei express the genetic information from a streamlined version of the genome but are replaced at each sexual generation. The macronuclear genome of Paramecium tetraurelia was recently sequenced by a shotgun approach, providing access to the gene repertoire. The 72-Mb assembly represents a consensus sequence for the somatic DNA, which is produced after sexual events by reproducible rearrangements of the zygotic genome involving elimination of repeated sequences, precise excision of unique-copy internal eliminated sequences (IES), and amplification of the cellular genes to high copy number. We report use of the shotgun sequencing data (>10 6 reads representing 13× coverage of a completely homozygous clone) to evaluate variability in the somatic DNA produced by these developmental genome rearrangements. Although DNA amplification appears uniform, both of the DNA elimination processes produce sequence heterogeneity. The variability that arises from IES excision allowed identification of hundreds of putative new IESs, compared to 42 that were previously known, and revealed cases of erroneous excision of segments of coding sequences. We demonstrate that IESs in coding regions are under selective pressure to introduce premature termination of translation in case of excision failure.

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Section: Germline Chromosomessupporting

confidence: 78%

Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: A somatic view of the germline

Duret¹,

Cohen²,

Jubin³

et al. 2008

Genome Res.

112

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“…1A; refs. [17][18][19]. This mechanism seems to be shared by other unicellular eukaryotes, as short IR sequences also facilitate palindrome formation in yeast, either following a DSB (20) or when MRE11 is mutated (21).…”

mentioning

confidence: 92%

Short inverted repeats initiate gene amplification through the formation of a large DNA palindrome in mammalian cells

Tanaka

Tapscott

Trask

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

108

105

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Gene amplification is a common form of genomic instability in a wide variety of organisms and is often associated with tumor progression in mammals. One striking feature of many amplified genes is their organization as large inverted duplications (palindromes). Here, we describe a molecular mechanism for palindrome formation in mammalian cells that is also conserved in protists. We introduced a short (79 or 229 bp) inverted repeat into the genome of Chinese hamster ovary cells and showed that it promoted the formation of a large DNA palindrome after an adjacent DNA double-strand break. This finding suggests that short inverted repeats in the mammalian genome can have a critical role in the initiation of gene amplification. This specific mechanism may provide a novel target for cancer therapies.

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“…The wild-type 156 and s mutant strains have the same micronuclear genome, yet telomeres rcproducibly form at different positions of the G gene sequence at each autogamy. Whether or not the G gene macronuclear telomere is produced by a breakage of the micronuclear chromosome during macronuclear development, the different telomere positions in the two strains cannot be determined by a cis-acting element such as the chromosome breakage sequence (Cbs) recently identified in Tetrahymena (Yao et al 1990). Clearly, in this case, another mechanism is involved in the positioning of the telomeres, which is determined by the old macronucleus rather than by the micronuclear sequence.…”

Section: Influence Of the Macronucleus On The Processing Of The Macromentioning

confidence: 99%

Induction of specific macronuclear developmental mutations by microinjection of a cloned telomeric gene in Paramecium primaurelia.

Meyer¹

1992

Genes Dev.

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The controlling sequence for site-specific chromosome breakage in tetrahymena

Cited by 125 publications

References 28 publications

Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: A somatic view of the germline

Analysis of sequence variability in the macronuclear DNA of Paramecium tetraurelia: A somatic view of the germline

Short inverted repeats initiate gene amplification through the formation of a large DNA palindrome in mammalian cells

Induction of specific macronuclear developmental mutations by microinjection of a cloned telomeric gene in Paramecium primaurelia.

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