The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers

Barrias, Cristina C.; Martins, M. Cristina L.; Almeida-Porada, Graça; Barbosa, Mário A.; Granja, Pedro L.

doi:10.1016/j.biomaterials.2008.09.048

Cited by 145 publications

(116 citation statements)

References 30 publications

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“…The adsorption of matrix proteins such as fibronectin (FN) on substrate surfaces has been shown to be of large importance when culturing a variety of cells in vitro [10][11].…”

Section: Introductionmentioning

confidence: 99%

Fibronectin adsorption and cell response on electroactive poly(vinylidene fluoride) films

et al. 2012

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Due to the large potential of electroactive materials in novel tissue engineering strategies, the aim of this work is to determine if the crystalline phase and/or the surface electrical charge of electroactive poly(vinylidene fluoride), PVDF, have influence on the biological response in monolayer cell culture. Non-polar α-PVDF and electroactive β-PVDF were prepared. The β-PVDF films were poled by corona discharge to show negative or positive electrical surface charge density. It has been concluded that hydrophilicity of the PVDF substrates depends significantly on crystalline phase and

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“…The adsorption of matrix proteins such as fibronectin (FN) on substrate surfaces has been shown to be of large importance when culturing a variety of cells in vitro [10][11].…”

Section: Introductionmentioning

confidence: 99%

Fibronectin adsorption and cell response on electroactive poly(vinylidene fluoride) films

et al. 2012

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“…This magnitude increases as the fraction of hydroxyl groups on the surface does. As we observed with the FN western blot, the amount of adsorbed FN on mixed CH 3 /OH surfaces is lower as the fraction of hydroxyl terminated chains increases ( Figure 8) and this is in agreement with results obtained on this family of SAMs by radiolabeling the protein (Barrias et al, 2009). That is to say, it is known that FN is adsorbed in higher amount on hydrophobic (CH 3 ) surfaces than hydrophilic ones (OH) ).…”

Section: Fibronectin Adsorptionsupporting

confidence: 79%

Material-driven fibronectin fibrillogenesis to engineer cell function.

Hernández¹

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“…All three proteins are known to adhere to biomaterial surfaces, and fibronectin in particular is thought to be an important regulator of cellular response [5,7,14,[108][109][110]. As well as measuring the protein adhesion at physiological conditions, pH = 7.4, the protein adhesion was also measured at pH = 6.2.…”

Section: Using Protein-functionalized Atomic Force Microscopy Tips Tomentioning

confidence: 99%

Designing nanostructured block copolymer surfaces to control protein adhesion

Schricker

Palacio

Bhushan

2012

Phil. Trans. R. Soc. A.

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The profile and conformation of proteins that are adsorbed onto a polymeric biomaterial surface have a profound effect on its in vivo performance. Cells and tissue recognize the protein layer rather than directly interact with the surface. The chemistry and morphology of a polymer surface will govern the protein behaviour. So, by controlling the polymer surface, the biocompatibility can be regulated. Nanoscale surface features are known to affect the protein behaviour, and in this overview the nanostructure of self-assembled block copolymers will be harnessed to control protein behaviour. The nanostructure of a block copolymer can be controlled by manipulating the chemistry and arrangement of the blocks. Random, A-B and A-B-A block copolymers composed of methyl methacrylate copolymerized with either acrylic acid or 2-hydroxyethyl methacrylate will be explored. Using atomic force microscopy (AFM), the surface morphology of these block copolymers will be characterized. Further, AFM tips functionalized with proteins will measure the adhesion of that particular protein to polymer surfaces. In this manner, the influence of block copolymer morphology on protein adhesion can be measured. AFM tips functionalized with antibodies to fibronectin will determine how the surfaces will affect the conformation of fibronectin, an important parameter in evaluating surface biocompatibility.

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The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers

Cited by 145 publications

References 30 publications

Fibronectin adsorption and cell response on electroactive poly(vinylidene fluoride) films

Fibronectin adsorption and cell response on electroactive poly(vinylidene fluoride) films

Material-driven fibronectin fibrillogenesis to engineer cell function.

Designing nanostructured block copolymer surfaces to control protein adhesion

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