The Correlation Between TNF-α Promoter Gene Polymorphism and Genetic Susceptibility to Cervical Cancer

Abstract

To investigate the association between the susceptibility to cervical cancer and the single nucleotide polymorphisms of 5 tumor necrosis factor-α promoter genes (rs361525, rs1800629, rs1800750, rs1799964, and rs673) in Chinese women. A total of 946 peripheral blood samples were collected from women of Han Ethnicity in Shandong province. Of them, 452 were diagnosed with cervical squamous cell carcinomas. The study also included a control group of 494 healthy women. The targeted single nucleotide polymorphisms were analyzed by TaqMan probe method. (1) The rate of high-risk subtype human papillomavirus infection in exfoliated cervical epithelial cells was significantly higher in patients with cervical cancer than the control group (91.4% vs 10.3%, P < .01). The rate of human papillomavirus infection was lower in patients with carcinoma in situ than those with invasive carcinoma (77.9% vs 95.4%, P < .01). (2) There was a significant difference for rs361525 genotype (CC/CT/TT) between the control, carcinoma in situ, and invasive carcinoma groups (P < .001). Both rs1800629 and rs1799964 genotypes (both GG/GA/AA) were also different between these groups (P < .001 and P < .001). (3) The allele frequencies of rs361525, rs1800629, and rs1799964 were significantly correlated with the diagnosis of cervical cancer. The frequency of T allele in rs361525 was significantly higher for cervical cancer group (10.8%) than control group (3.8%; odds ratio = 3.04, 95% confidence interval = 1.76-5.25, P < .01). The frequency of A allele in rs1800629 was significantly higher for cervical cancer (29.9%) than control group (14.2%; odds ratio = 2.58, 95% confidence interval = 1.87-3.56, P < .01). The frequency of A allele in rs1799964 was also higher for cervical cancer group (38.3%) than control group (16.4%; odds ratio = 1.43, 95% confidence interval = 1.07-1.91, P < .05). The rs361525, rs1800629, and rs17999645 were significantly correlated with the diagnosis of cervical cancer.

Association of TGF-β1 and TNF-α genes polymorphisms with the kidney scars forming risk in children with vesicoureteral reflux

“…A significant link was observed in the case of TNF-α -308 G/A, -238 C/T and -1031 G/A polymorphisms with an increased risk of cervical cancer (LI et al, 2018).…”

The association of TNF-α promoter polymorphisms with genetic susceptibility to cervical cancer in a Chinese Han population

“…showed that the A/A and G/A-A/A genotypes of rs361525 are related to the progression of CIN but not to the eventual occurrence of cervical cancer in Tunisia [41]. In 2018, Li et al reported that the rs361525 A allele is a risk factor for cervical cancer in a Chinese population from Shandong Province, northern China [18], and in 2019, Du et al showed that it is a protective factor against cervical cancer in Sichuan Province, southwestern China [12]. In the current study, the A allele of rs361525 was found to be a risk factor for cervical cancer in a Chinese population.…”

“…For example, in 2001, Jang et al reported that the A allele of rs361525 is a protective factor for cervical cancer in a Korean population [14]. However, in 2018, Li et al reported that the A allele of rs361525 is a risk factor for squamous cell carcinoma in a Chinese population from Shandong Province, northern China [18]. Thus, more populations from different regions should be investigated.…”

Polimorfismo -308 del gen TNFa y su expresión local en lesiones preinvasivas y cáncer de cuello uterino en el estado Zulia, Venezuela

“…Aunque, los hallazgos señalan el VPH como un factor de riesgo importante (3), no es causa suficiente para malignidad, ya que gran número de mujeres infectadas con el VPH nunca desarrollaron lesiones cervicales (8). Esto puede sugerir la implicación de los antecedentes genéticos en la transformación maligna de células epiteliales a cáncer de cuello uterino (9). La respuesta inmunitaria a nivel del cuello uterino es compleja, principalmente debido a su participación en los eventos reproductivos.…”

“…Algunos investigadores sugieren que el desarrollo de tumores puede estar relacionado a la predisposición genética del individuo de producir mayores niveles de TNFα. Actualmente, existen estudios enfocados en los efectos de variaciones en la región promotora del gen TNFa y su relación con el cáncer de cuello uterino, encontrándose resultados contradictorios (9,17,(20)(21)(22)(23). Sin embargo, otros autores sugieren que la diferencia entre los niveles de producción de TNFα radica en la base genética de las poblaciones estudiadas (23,24).…”