The Correlative Light and Electron Microscopy of the Islets of Langerhans in Some Lower Vertebrates

Watari, Nakazo; Tsukagoshi, Noboru; Honma, Yoshio

doi:10.1679/aohc1950.31.371

Cited by 41 publications

(12 citation statements)

References 27 publications

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“…In contrast to these findings, Trandaburu and Calugareanu (1969) reported five cell types by light microscopy and six cell types by electron microscopy. Conversely, Watari et al (1970) observed only A and B cells. Unfortunately, the review by Miller and Lagios (1970) did not resolve the question of the endocrine cell types present in the ophidian pancreas.…”

Section: Discussionmentioning

confidence: 90%

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Rhoten

1984

Anat. Rec.

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Insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) were localized in the pancreas of the common garter snake, Thamnophis sirtalis, by light and transmission electron microscopic (TEM) immunocytochemistry. Colloidal gold-protein A was used for TEM localization and the peroxidase--antiperoxidase complex technique was used for light microscopy. The glucagon-containing A cells and the insulin-positive B cells were the most numerous cell types. The somatostatin-containing D cells made up about 15% of the endocrine cells. PP-positive F cells were a minor cell type. The only topographic arrangement of the cells within the endocrine-rich areas that was apparent was the peripheral localization of the D and F cells. Cells of a specific cell type were sometimes grouped together. At the electron microscopic (EM) level, the gold particles (indicating the presence of hormone) were localized nearly exclusively over the secretory granules of the reactive cells. The alpha-granules were the largest found and were predominantly electron dense with a moderately electron-dense periphery. PP-containing granules were the smallest. The somatostatin-reactive delta-granules were round and moderately electron opaque. The beta-granules were heterogeneous in appearance. The morphognomy of the secretory granules of the major endocrine cell types is qualitatively similar to that of mammals. Whether or not the quantitative and/or associative differences contribute to the marked metabolic differences between reptiles and mammals, remains to be determined.

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Section: Discussionmentioning

confidence: 90%

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Rhoten

1984

Anat. Rec.

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“…An excretory ductule surrounded by several endocrine cells were observed in the islet of elasmobranchs and snake20, 21,26), and in early development in anura"), avian"), me), bovine") and human embryos9). The pancreatic ductular epithelium of the rabbit was observed to be contiguous with or within islets and new formation of B cells from duct epithelium could be traced under long term hyperglycemic status8'24).…”

Section: Discussionmentioning

confidence: 99%

Electron Microscopic Study of Intercalated Duct Cells in the Chicken Pancreatic Islet and Effects of Tolbutamide Administration

Mutoh

Wakuri

Liu

et al. 1998

Okajimas Folia Anatomica Japonica

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“…Some investigators (FUJITA and MATSUNO, 1967;WATARI et al, 1970) postulated that the second type of B granule release might be diacrinous secretion. MIKAMI and MUTOH (1971) reported that the B granule release might occur very rapidly or only during a limited period of time in the chick.…”

Section: Discussionmentioning

confidence: 99%

Fine Structure of the Pancreatic Islets in Domestic Fowl with Special Reference to the Cell Type and Secretion

Watanabe

Paik

Yasuda

1975

Arch. Histol. Jap., Arch. Histol. Jpn

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The Correlative Light and Electron Microscopy of the Islets of Langerhans in Some Lower Vertebrates

Cited by 41 publications

References 27 publications

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis

Electron Microscopic Study of Intercalated Duct Cells in the Chicken Pancreatic Islet and Effects of Tolbutamide Administration

Fine Structure of the Pancreatic Islets in Domestic Fowl with Special Reference to the Cell Type and Secretion

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