Reading comprehension is a complex cognitive process involving many human brain activities. Plenty of works have studied the reading patterns and attention allocation mechanisms in the reading process. However, little is known about what happens in human brain during reading comprehension and how we can utilize this information as implicit feedback to facilitate information acquisition performance. With the advances in brain imaging techniques such as electroencephalogram (EEG), it is possible to collect highprecision brain signals in almost real time.With neuroimaging techniques, we carefully design a lab-based user study to investigate brain activities during reading comprehension. Our findings show that neural responses vary with different types of contents, i.e., contents that can satisfy users' information needs and contents that cannot. We suggest that various cognitive activities, e.g., cognitive loading, semantic-thematic understanding, and inferential processing, at the micro-time scale during reading comprehension underpin these neural responses. Inspired by these detectable differences in cognitive activities, we construct supervised learning models based on EEG features for two reading comprehension tasks: answer sentence classification and answer extraction. Results show that it is feasible to improve their performance with brain signals. These findings imply that brain signals are valuable feedback for enhancing human-computer interactions during reading comprehension.