The crayfish <i>Procambarus clarkii</i> CRY shows daily and circadian variation

Fanjul‐Moles, María Luisa; Escamilla‐Chimal, Elsa G.; Gloria‐Soria, Andrea; Hernández-Herrera, Gabriela

doi:10.1242/jeb.00900

Cited by 39 publications

(37 citation statements)

References 28 publications

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“…In addition, its presence as a single CRY-ir protein only and of a size similar to that of Drosophila CRY has been confirmed by Western blotting of tissue extracts (339). Whilst already assumed in the latter study, a clear-cut association of crayfish CRY with the extraretinal BPRs within the AMC cluster 6 has only recently been described for the crayfish C. destructor and P. clarkii (17) (cp.…”

Section: Crustacean Cryptochrome-genementioning

confidence: 60%

“…In the adult brain, CRY levels change with a circadian rhythm (but only in the AMC), which was considered suggestive of a possible function of CRY in crayfish circadian control. In eyestalk locations of the adult, apparently no significant circadian changes of CRY do occur (339). However, in the eyestalk of second post-embryonic stages (PO2) under LD conditions, CRY expression oscillates with a bimodal pattern but somewhat in antiphase to that in the brain (Eyestalk: high at 15:00ZT, low at 23:00 ZT; brain: low at 11:00ZT and high at 23:00ZT).…”

Section: Crustacean Cryptochrome-genementioning

confidence: 99%

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Circadian clocks in crustaceans: identified neuronal and cellular systems

Strauß

Dircksen²

2010

Front Biosci

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Circadian rhythms are known for locomotory and reproductive behaviours, and the functioning of sensory organs, nervous structures, metabolism and developmental processes. The mechanisms and cellular bases of control are mainly inferred from circadian phenomenologies, ablation experiments and pharmacological approaches. Cellular systems for regulation summarised here comprise the retina, the eyestalk neuroendocrine X-organ-sinus gland system, several neuropeptides such as red pigment concentrating, hyperglycaemic and pigment-dispersing hormones, and factors such as serotonin and melatonin. No master clock has been identified, but a model of distributed clockwork involves oscillators such as the retinular cells, neurosecretory systems in the optic lobes, putative brain pacemakers, and the caudal photoreceptor. Extraretinal brain photoreceptors mediate entrainment. Comparative analyses of clock neurons and proteins known from insects may allow the identification of candidate clock neurons in crustaceans as putative homologues in the two taxa. Evidence for the existence of "insect-like" intracellular clock proteins and (light sensitive) transcription factors is scarce, but clock-, period-, and cryptochrome-gene products have been localised in the CNS and other organs rendering further investigations into crustacean clockwork very promising.

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Section: Crustacean Cryptochrome-genementioning

confidence: 60%

Section: Crustacean Cryptochrome-genementioning

confidence: 99%

Circadian clocks in crustaceans: identified neuronal and cellular systems

Strauß

Dircksen²

2010

Front Biosci

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“…Blue light is present over the entire twilight depth range (Jerlov 1968) and likely entrains the diel rhythms of benthic decapods distributed on shelves and slopes (Aguzzi and Company 2010). This hypothesis is supported by the presence of blue light photoreceptors in crayfish (Fanjul-Moles et al 2005) and the ubiquity of such receptors in the animal kingdom (Hankins et al 2008).…”

Section: Methodsmentioning

confidence: 99%

Dusk but not dawn burrow emergence rhythms of <i>Nephrops norvegicus</i> (Crustacea: Decapoda)

Sbragaglia¹,

Aguzzi²,

García³

et al. 2013

Sci. Mar.

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SUMMARY: The Norway lobster, Nephrops norvegicus, can be captured by haul nets only during the emergence from its burrow. In the last few decades, an extensive field research revealed distinct diel (24-h-based) catchability patterns at different depths. Laboratory experiments suggested that burrow emergence (used as a proxy of catchability) is endogenously controlled via a circadian system. Results were usually presented in terms of mean effects without a quantification of interindividual variability and arrhythmia. Here, we studied the burrow emergence of 52 adult Nephrops by an infrared actograph endowed with an artificial burrow. Animals were exposed to 12-12 h light-darkness cycle, simulating photic condition of the lower shelf. Forty-five animals showed rhythmic emergence (87%), while seven were arrhythmic (13%). Rhythmic animals were clustered according to their timing of emergence: 54% at dusk and 4% at dawn. Moreover, other animals showed fully diurnal or nocturnal emergence (10% and 19%, respectively). The comparison of our results with those derived from temporally scheduled trawling indicates that bimodal catch patterns observed in shelf populations are poorly observed during individual experiments in the laboratory, where the same light conditions are simulated. Nephrops burrow emergence seems to be the result of a mixed endogenous-exogenous control, while arrhythmia could also be present in the wild.Keywords: Nephrops norvegicus, circadian system, emergence behaviour, dusk and dawn, arrhythmia.RESUMEN: Prevalencia del ritmo de emergencia al anochecer frente al amanecer en la cigala Nephrops Norvegicus (Crustacea: Decapoda). -La cigala, Nephrops norvegicus, es capturada con arte de arrastre cuando emerge de su madriguera. En las últimas décadas, una extensa investigación de campo ha revelado distintos patrones diarios (24-h) de capturas en diferentes profundidades. Se ha observado en experimentos de laboratorio que la emergencia desde la madriguera (interpretable como capturabilidad), está bajo el control endógeno de un sistema circadiano. En estos estudios los resultados se han presentado como efecto promedio en la población experimental, sin una cuantificación de la variabilidad inter individual o del porcentaje de arritmicidad. En este trabajo se estudió la actividad de emergencia desde la madriguera de Nephrops en 52 animales mediante un actógrafo de infrarrojos en un tanque dotado de una madriguera artificial, con un fotoperiodo de 12-12 luz-oscuridad, a fin de simular las condiciones fóticas de la plataforma marina. Cuarenta y cinco animales mostraron una emergencia rítmica (87%), mientras que siete fueron arrítmicos (13%). Los animales rítmicos fueron agrupados de acuerdo al momento de emergencia: 54% al "atardecer" y 4% al "amanecer", mientras que otros mostraron una emergencia totalmente diurna o nocturna (10% y 19%, respectivamente). La comparación de nuestros resultados con los datos procedentes de pescas experimentales de arrastre, indica que los patrones bimodales de captura observados e...

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“…This suggests that both proteins in crayfish are transcription modulators. Previous research from our laboratory has demonstrated the presence of another circadian protein, CRY, in the brain of crayfish, with the CRY cycle peaking at night (Fanjul-Moles et al, 2004). Hence, although some authors have recently demonstrated that CRY is linked to photoreception of the clock in crayfish (Sullivan et al, 2009), the possibility exists that, as with insects, CRY in crayfish functions pleiotropically in circadian rhythm generation.…”

mentioning

confidence: 95%

Putative pacemakers of crayfish show clock proteins interlocked with circadian oscillations

Escamilla‐Chimal

Velázquez-Amado

Fiordelisio

et al. 2010

Journal of Experimental Biology

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SUMMARYAlthough the molecular mechanisms that control circadian rhythms in many animals, particularly in the fly, are well known, molecular and biochemical studies addressing the location and function of the proteins and genes contributing to the cycling of the clock in crayfish Procambarus clarkii are scarce. In this study, we investigated whether three proteins that interact in the feedback loop of the molecular clock described for Drosophila are expressed in the putative circadian pacemakers of crayfish retina, eyestalk and brain and whether their expression cycles in a manner consistent with elements of the circadian clock. Here we identified PER, TIM and CLK immunoreactivity in the cytoplasm and nucleus of cells located in the retina as well as in clusters of cells and neuropils of the optic ganglia, lateral protocerebrum and brain. Brain clusters 6, 10, 9 and 11, in particular, showed Per, Tim and Clk-like immunoreactivity at the perikarya and nucleus, and these antigens colocalized at Zeitgeber time (

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The crayfish Procambarus clarkii CRY shows daily and circadian variation

Cited by 39 publications

References 28 publications

Circadian clocks in crustaceans: identified neuronal and cellular systems

Circadian clocks in crustaceans: identified neuronal and cellular systems

Dusk but not dawn burrow emergence rhythms of <i>Nephrops norvegicus</i> (Crustacea: Decapoda)

Putative pacemakers of crayfish show clock proteins interlocked with circadian oscillations

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