2018
DOI: 10.1111/jipb.12734
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The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Abstract: Since the discovery that nucleases of the bacterial CRISPR (clustered regularly interspaced palindromic repeat)‐associated (Cas) system can be used as easily programmable tools for genome engineering, their application massively transformed different areas of plant biology. In this review, we assess the current state of their use for crop breeding to incorporate attractive new agronomical traits into specific cultivars of various crop plants. This can be achieved by the use of Cas9/12 nucleases for double‐stra… Show more

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Cited by 102 publications
(65 citation statements)
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References 232 publications
(323 reference statements)
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“…The emergence of the CRISPR/Cas technology enabled targeted double‐strand break (DSB) induction at high efficiency in a simple way (Jinek et al ., ; Le Cong et al ., ). Since then, a broad range of new applications of the technology is currently transforming plant biology (Baltes et al ., ; Malzahn et al ., ; Puchta, ; Kumlehn et al ., ; Langner et al ., ; Chen et al ., ; Sedeek et al ., ; Wolter et al ., ). The stimulatory potential of targeted DSB induction for non‐homologous end‐joining (NHEJ)‐mediated gene knockout and homologous recombination (HR)‐mediated gene targeting (GT) in plants was already demonstrated long before the development of CRISPR/Cas (Puchta et al ., ; Salomon and Puchta, ).…”
Section: Introductionmentioning
confidence: 99%
“…The emergence of the CRISPR/Cas technology enabled targeted double‐strand break (DSB) induction at high efficiency in a simple way (Jinek et al ., ; Le Cong et al ., ). Since then, a broad range of new applications of the technology is currently transforming plant biology (Baltes et al ., ; Malzahn et al ., ; Puchta, ; Kumlehn et al ., ; Langner et al ., ; Chen et al ., ; Sedeek et al ., ; Wolter et al ., ). The stimulatory potential of targeted DSB induction for non‐homologous end‐joining (NHEJ)‐mediated gene knockout and homologous recombination (HR)‐mediated gene targeting (GT) in plants was already demonstrated long before the development of CRISPR/Cas (Puchta et al ., ; Salomon and Puchta, ).…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, “speed breeding” technology can not only be applied for faster population development but certainly also for generation advancement of transgenic plants. Most importantly, wheat and barley are similarly as amenable to gene editing methods as other crop and non‐crop species (Kumlehn et al ), providing the important genetic basis for efficient crop breeding and functional gene or allele characterization.…”
Section: Promising Research Areas For the Post‐genomics Eramentioning
confidence: 99%
“…Some Cas9-derived base editor systems have also been established by fusing an adenine or a cytidine deaminase to a Cas9n protein [109]. Being different from the classical Cas9 system, the base editor systems can catalyze two kinds of nucleotide replacement reactions, from adenine (A) to guanine (G) or from cytosine (C) to thymine (T), depending on the deaminase that is linked to Cas9n [109].…”
Section: Crispr/cas9 Systemmentioning
confidence: 99%