The crude extract of<i>Corni Fructus</i>inhibits the migration and invasion of U-2 OS human osteosarcoma cells through the inhibition of matrix metalloproteinase-2/-9 by MAPK signaling

Liao, Ching Lung; Lin, Jing‐Yuan; Lien, Jin‐Cherng; Hsu, Shu Chun; Chueh, Fu Shin; Yu, Chih Chieh; Huang, Yi Ping; Lin, Jaung Geng; Chung, Jing Gung

doi:10.1002/tox.21894

Cited by 32 publications

(33 citation statements)

References 41 publications

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“…Using a type 2 DM model, this paper explored the role of PC on WT1 expression in glomeruli and the mechanism of PC on early nephropathy. We found that PC had the following effects: (1) it significantly reduced the FBG and elevated INS levels; (2) it had the effect of protecting against the renal damage, such as reducing the NAG and mALB levels, the renal morphology score and the kidney coefficient; and (3) it enhanced WT1 expression in glomerular podocytes. The findings indicated that PC had the effect of protecting early nephropathy in type 2 diabetic rats.…”

Section: Corni Fructus Components On Early Diabetic Nephropathy 545mentioning

confidence: 83%

Effect of the Best Compatibility of Components in Corni Fructus on WT1 Expression in Glomerular Podocytes of Type 2 Diabetic Rats with Early Nephropathy

Liu

Shen

et al. 2012

Am. J. Chin. Med.

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Our previous studies have shown that the best compatibility of components in Corni Fructus (PC) had a protective effect on vascular endothelial cells. In this study, the effect of PC on WT1 expression in glomerular podocytes and the mechanism of PC on early nephropathy in type 2 diabetic rats have been investigated. Type 2 diabetic rats were generated by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin (STZ), then fed aminoguanidine (100 mg/kg), glimepiride (0.4 mg/kg), low-dose (60 mg/kg) or high-dose (120 mg/kg) of PC once a day for 12 weeks. Fasting blood glucose (FBG) was examined regularly. Insulin (INS) was measured by a radioimmunoassay. Microalbuminuria (mALB) was measured by an ELISA assay. Urinary creatinine (UCr), blood urea nitrogen (BUN) and N-acetyl-β-D-glucosaminidase (NAG) were measured by colorimetric assays. Renal morphological changes were observed by optical microscopy. The expression index of Wilms tumor 1 (WT1EI) in glomeruli was examined by immunohistochemistry. The results showed that PC led to a decrease in FBG (p < 0.01), NAG (p < 0.05) and mALB (p < 0.05) and an increase in INS (p < 0.01) and WT1EI (p < 0.05) compared to the diabetic group. PC could improve renal damage and greatly reduce the renal morphology score (p < 0.05). These results suggested that PC had the protective effect on early nephropathy in type 2 diabetic rats, which was closely related to the regulation of podocytes.

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Section: Corni Fructus Components On Early Diabetic Nephropathy 545mentioning

confidence: 83%

Effect of the Best Compatibility of Components in Corni Fructus on WT1 Expression in Glomerular Podocytes of Type 2 Diabetic Rats with Early Nephropathy

Liu

Shen

et al. 2012

Am. J. Chin. Med.

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“…U‐2 OS cell line was purchased from the Food Industry Research and Development Institute (Hsinchu, Taiwan). Cells were cultured in 90% McCoy's 5A medium with 10% FBS, 2 mM L‐glutamine and antibiotics (100 Units/ml penicillin, and 100 μg/ml streptomycin) in a 75 cm 2 tissue culture flask at 37°C in a humidified atmosphere of 5% CO 2 …”

Section: Methodsmentioning

confidence: 99%

“…U‐2 OS cells (1.6 × 10 5 cells/well) were placed in a 12‐well plate for overnight then incubated with or without ouabain. Cells were collected, counted and stained with PI (5 μg/ml) and were analyzed using flow cytometer (BD Biosciences, FACS Calibur, San Jose, CA) assay for total viable cell number as previously described …”

Section: Methodsmentioning

confidence: 99%

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Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U‐2 OS cells

Shih¹,

Liu³

et al. 2017

Environmental Toxicology

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Ouabain, the specific Na /K -ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies. Ouabain inhibited cell migration, invasion and the enzymatic activities of MMP-2, and also affected the expression of metastasis-associated protein in U-2 OS cells. The cDNA microarray assay indicated that CDH1, TGFBR3, SHC3 and MAP2K6 metastasis-related genes were increased, but CCND1, JUN, CDKN1A, TGFB1, 2 and 3, SMAD4, MMP13, MMP2 and FN1 genes were decreased. These findings provide more information regarding ouabain inhibited cell migration and invasion and associated gene expressions in U-2 OS cells after exposed to ouabain.

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“…A previous study reported that oroxylin A could inhibit migration and invasion of human breast MDA-MB-435 cancer cells via the inhibition of MMP-2 and MMP-9 (11). Thus, MMP-2 and MMP-9 have been considered as targets in the development of drugs against tumor invasion and metastasis (12,13).…”

Section: Introductionmentioning

confidence: 98%

Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9

Zhang

Han

Zhai

et al. 2012

Molecular Medicine Reports

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Ophiopogonin-D is one of steroidal saponins isolated from the root of the Chinese medicinal plant Ophiopogon japonicas. It has been claimed to possess anti-inflammatory and anti-oxidant properties. The present study was the first to examine the anti-tumor metastasis properties of ophiopogonin-D. An MTT assay showed that ophiopogonin-D inhibited the proliferation of MDA-MB-435 melanoma cells, and decreased invasion was demonstrated using a Transwell invasion assay. Furthermore, adhesion of MDA-MB-435 cells to human umbilical vascular endothelial cells and to fibronectin was inhibited by ophiopogonin-D. Gelatin zymography and western blot analysis showed that ophiopogonin-D inhibited the expression and secretion of matrix metalloproteinase-9 (MMP-9), but not that of MMP-2. Inhibition of phosphorylation of p38 by ophiopogonin-D indicated its inhibition of the mitogen-activated protein kinase pathway. Overall, the results suggested that ophiopogonin-D may be considered as a candidate drug for treating or preventing tumor metastasis.

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The crude extract ofCorni Fructusinhibits the migration and invasion of U-2 OS human osteosarcoma cells through the inhibition of matrix metalloproteinase-2/-9 by MAPK signaling

Cited by 32 publications

References 41 publications

Effect of the Best Compatibility of Components in Corni Fructus on WT1 Expression in Glomerular Podocytes of Type 2 Diabetic Rats with Early Nephropathy

Effect of the Best Compatibility of Components in Corni Fructus on WT1 Expression in Glomerular Podocytes of Type 2 Diabetic Rats with Early Nephropathy

Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U‐2 OS cells

Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9

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