The crystal-forming strains of Bacillus laterosporus

Смирнова, Т. А.; Minenkova, I.B.; Orlova, Margarita V.; Lecadet, Marguerite-M.; Azizbekyan, R. R.

doi:10.1016/0923-2508(96)84709-7

Cited by 35 publications

(25 citation statements)

References 5 publications

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“…In this context, strains of B. laterosporus with and without parasporal bodies have been reported to show biocidal activity towards mosquito larvae. In particular, the strains 16-92 (921) and LAT 006 (615) showed significant activity against larvae of Aedes aegypti and Anopheles stephensis (21,34). It was demonstrated that both mosquitocidal strains produced parasporal crystals with different sizes and shapes which were believed to be responsible for the observed toxic activity, although no information was provided as to the composition of these structures.…”

Section: Resultsmentioning

confidence: 99%

“…b For more details about the strains, see the text and references (6,8,16,21,31,34,41 (38), with the application of a hot start (7 min at 95°C) to avoid initial mispriming and to enhance the specificity. Amplification of the variable spacer region between the 16S and 23S gene sequences was performed by established methods for intergenic transcribed spacer PCR (ITS-PCR) (10), utilizing 25 ng of genomic DNA as a template.…”

Section: Methodsmentioning

confidence: 99%

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Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control

Oliveira¹,

Rabinovitch²,

Monnerat

et al. 2004

Appl Environ Microbiol

129

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Thirty-three strains of Brevibacillus laterosporus, including three novel strains isolated from Brazilian soil samples, were examined for genetic variability by the use of different PCR-based methods. Molecular markers that could characterize bacterial strains with regards to their pathogenic potential were investigated. In addition, toxicity was assessed by the use of insects belonging to the orders Lepidoptera and Coleoptera and the mollusk Biomphalaria glabrata. Among the targets tested, Biomphalaria glabrata demonstrated the highest degree of sensitivity to B. laterosporus, with some strains inducing 90 to 100% mortality in snails aged 3 and 12 days posteclosion. Larvae of the coleopteron Anthonomus grandis were also susceptible, presenting mortality levels of between 33 and 63%. Toxicity was also noted towards the lepidopteron Anticarsia gemmatalis. In contrast, no mortality was recorded among test populations of Tenebrio molitor or Spodoptera frugiperda. The application of intergenic transcribed spacer PCR and BOX-PCR generated 15 and 17 different genotypes, respectively. None of the molecular techniques allowed the identification of a convenient marker that was associated with any entomopathogenic phenotype. However, a 1,078-bp amplicon was detected for all strains of B. laterosporus when a primer for amplification of the BOXA1R region was used. Similarly, a 900-bp amplicon was generated from all isolates by use of the primer OPA-11 for randomly amplified polymorphic DNA analysis. These amplicons were not detected for other phenotypically related Brevibacillus species, indicating that they represent markers that are specific for B. laterosporus, which may prove useful for the isolation and identification of new strains of this species.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control

Oliveira¹,

Rabinovitch²,

Monnerat

et al. 2004

Appl Environ Microbiol

129

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“…The B. laterosporus strain A60 has a well-known ability to act as a biocontrol agent, to induce systemic resistance in plants, and to produce various antagonistic factors, such as a parasporal crystal, an extracellular protease, a lipopeptide antibiotic, and a pseudopeptide (35,(54)(55)(56). However, the molecular mechanisms involved in the activation of systemic resistance by strain A60 have not yet been completely elucidated.…”

Section: Discussionmentioning

confidence: 99%

PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

Wang

Yang

Guo

et al. 2015

Appl Environ Microbiol

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We report the identification, characterization, and gene cloning of a novel protein elicitor (PeBL1) secreted from Brevibacillus laterosporus strain A60. Through a purification process consisting of ion-exchange chromatography and high-performance liquid chromatography (HPLC), we isolated a protein that was identified by electrospray ionization quadrupole time of flight tandem mass spectrometry ( , and PAL were upregulated to varying degrees by PeBL1. This research not only provides insights into the mechanism by which beneficial bacteria activate plant systemic resistance, but also sheds new light on a novel strategy for biocontrol using strain A60.

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“…aegypti and A. stephensi, and less active against C. pipiens. Both produce crystalline inclusions of various shapes and sizes (Smirnova et al 1996) responsible for the toxicity. Purified B. laterosporus 615 crystals are as toxic as those from Bti, to Ae.…”

Section: Brevibacillus Laterosporusmentioning

confidence: 99%

Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena

Charles

Nielsen-LeRoux

2000

Mem. Inst. Oswaldo Cruz

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The crystal-forming strains of Bacillus laterosporus

Cited by 35 publications

References 5 publications

Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control

Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control

PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena

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