The Crystallization of Sodium Hypochlorite on Gutta-percha Cones After the Rapid-Sterilization Technique: An SEM Study

Abstract

The purpose of this study was to identify the presence, crystallization, and subsequent removal of sodium hypochlorite crystals on gutta-percha cones after rapid sterilization. Seventy-two, fresh, standardized, gutta-percha cones were randomly selected. Each cone was observed under the scanning electron microscope and the elemental analysis machine before and after rapid sterilization using 5.25% and 2.5% sodium hypochlorite. Gutta-percha cones were rinsed after sterilization with 96% ethyl alcohol, 70% isopropyl alcohol, and distilled water independently to determine which of these agents removed the sodium-chloride crystals. There were no crystals present on the gutta-percha cones directly from the box. All gutta-percha cones had sodium-chloride crystals present after the rapid-sterilization technique using 5.25% and 2.5% sodium hypochlorite. However, the sodium-chloride crystals were removed by 96% ethyl alcohol, 70% isopropyl alcohol, and distilled water.

Effectiveness of different auxiliary chemical substances in the rapid disinfection of gutta-percha points - an in vitro study

“…However, gutta-percha points that are immersed in sodium hypochlorite solution formed chloride-crystal on its surface. These sodium hypochlorite crystals on the gutta-percha points impair the ability of the filling material to promote an adequate sealing 6 , which is a disadvantage of this kind of method for the rapid sterilization of gutta-percha points. Similarly, G3 (Ca(OCl) 2 ) and G4 (QMix) showed similar results to the 2.5% sodium hypochlorite and 2% chlorhexidine gel.…”

“…Due to its biological and physical properties, it is still an important dental material, emerging as the prime root canal filling material 5 . However, gutta-percha points are usually used directly from the package without regard to their sterility, and may showing contamination 6 . However, standard autoclave and high temperature sterilization methods deform this kind of material.…”

Efeito da desinfecção química nas superficies de guta-percha e Resilon

“…Em relação às alterações topográficas na superfície dos materiais devido ao uso das soluções descontaminantes, no presente estudo não foram observadas modificações na topografia. Estes resultados estão de acordo com estudos prévios que avaliaram os efeitos na topografia por MEV 2,4,6 . Entretanto diferem daqueles que utilizaram como técnica de análise a microscopia de força atômica (AFM) [8][9][10][11] .…”

“…Entretanto, a literatura é ainda bastante controversa nesse aspecto. Estudos utilizando microscopia eletrôni-ca e varredura (MEV) mostram, qualitativamente, não haver modificações topográficas na superfície desses materiais 2,4,6,7 . Ao passo que análises quantitativas, utilizando microscopia de força atômica, relatam modificações nessa topografia 8,11 .…”

Assessment of sterilization effect and the alteration of surface texture and physical properties of gutta-percha cone after short-term chemical disinfection

“…Because of its superior antibacterial effect, the sterilization of the GP cone with 5.25% NaOCl prior to canal filling has been recommended for a long time [4][5][6][7] . However, Short et al have shown that the cluster of cuboidal crystals on the surface of NaOClsoaked GP cone was detected at various levels and mentioned that at the time of canal filling, it might affect the apical sealing 14) . Our present study demonstrated not only cuboidal crystals but also round shaped structures that appeared to be the components of cone on the surface of NaOClsoaked GP cone under high magnification.…”