The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome.

López‐Juez, Enrique; Nagatani, Akira; Tomizawa, Kenji; Deák, Mária; Kern, Rainer; Kendrick, Richard E.; Furuya, Masaki

doi:10.1105/tpc.4.3.241

Cited by 136 publications

(43 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The difference in elongation rate between the hypocotyl of the lh mutant and its WT under low FR-containing fluorescent light is dramatic, making the seedlings amenable to yhysiological analysis. It appears that phyA is unaffected by the Ih mutation (López-Juez et al, 1992). The main endogenous active GA has been identified as GA, in cucumber (Nakayama et al, 1989(Nakayama et al, , 1991, with the main GA active in other species, GA,, also present at levels lower than GA, (Smith et al, 1991).…”

mentioning

confidence: 99%

“…Studies of the tall cucumber (Cucumis sativus L.) Z h mutant, whose seedlings are insensitive to a pulse of FR at the Present address: Plant Biology Laboratory, The Salk Institute, end of each day (Adamse et al, 1988), have led to the observation that the mutant displays a constitutive shadeavoidance reaction (López-Juez et al, 1990) and lacks a PHYB-like protein (López-Juez et al, 1992). Therefore, it is proposed that phyB plays a dominant role in the shadeavoidance reaction in cucumber.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Phytochrome, Gibberellins, and Hypocotyl Growth (A Study Using the Cucumber (Cucumis sativus L.) long hypocotyl Mutant)

et al. 1995

View full text Add to dashboard Cite

The possible involvement of gibberellins (CAs) in the regulation of hypocotyl elongation by phytochrome was examined. Under white light the tal1 long hypocotyl (Ih) cucumber (Cucumis sativus L.) mutant, deficient in a type B-like phytochrome, shows an increased "responsiveness" (defined as response capability) to applied CA, (the main endogenous active CA) compared to the wild type. Supplementing far-red irradiation results in a similar increase in responsiveness in the wild type. Experiments involving application of the precursor GA, and of an inhibitor of CA, inactivation suggest that both the GA, activation and inactivation steps are phytochrome independent. Endogenous C A levels of whole seedlings were analyzed by combined gas chromatography-mass spectrometry using deuterated interna1 standards. The levels of GA, (and those of CA,,, the inactivated CA,) were lower in the //I mutant under low-irradiance fluorescent light compared with the wild type, similar to wild type under higher irradiance light during the initial hypocotyl extension phase, and higher during the phase of sustained growth, in which extension involved an increase in the number of cells in the upper region. In all cases, growth of the Ih mutant was more rapid than that of the wild type. It is proposed that CA, and phytochrome control cell elongation primarily through separate mechanisms that interact at a step close to the terminal response.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Phytochrome, Gibberellins, and Hypocotyl Growth (A Study Using the Cucumber (Cucumis sativus L.) long hypocotyl Mutant)

et al. 1995

View full text Add to dashboard Cite

show abstract

“…Arabidopsis phyB, phyD, and phyE mutants have been isolated and used to demonstrate that all three function in low fluence, R/FR-reversible responses and in shade avoidance responses to altered R to FR ratio (Reed et al, 1993;Aukerman et al, 1997;Devlin et al, 1998Devlin et al, , 1999. Again, mutations in several PHYB-related genes from other plant species affect low fluence, R/FR-reversible responses and shade avoidance responses in a similar way (Lopez-Juez et al, 1992;Weller et al, 1995;Childs et al, 1997;Devlin et al, 1997;Lazarova et al, 1998b). Mutations in the Arabidopsis PHYC gene or in PHYC homologs from other species have not yet been described.…”

mentioning

confidence: 99%

Patterns of Expression and Normalized Levels of the Five Arabidopsis Phytochromes

2002

View full text Add to dashboard Cite

Using monoclonal antibodies specific for each apoprotein and full-length purified apoprotein standards, the levels of the five Arabidopsis phytochromes and their patterns of expression in seedlings and mature plants and under different light conditions have been characterized. Phytochrome levels are normalized to the DNA content of the various tissue extracts to approximate normalization to the number of cells in the tissue. One phytochrome, phytochrome A, is highly light labile. The other four phytochromes are much more light stable, although among these, phytochromes B and C are reduced 4- to 5-fold in red- or white-light-grown seedlings compared with dark-grown seedlings. The total amount of extractable phytochrome is 23-fold lower in light-grown than dark-grown tissues, and the percent ratios of the five phytochromes, A:B:C:D:E, are measured as 85:10:2:1.5:1.5 in etiolated seedlings and 5:40:15:15:25 in seedlings grown in continuous white light. The four light-stable phytochromes are present at nearly unchanging levels throughout the course of development of mature rosette and reproductive-stage plants and are present in leaves, stems, roots, and flowers. Phytochrome protein expression patterns over the course of seed germination and under diurnal and circadian light cycles are also characterized. Little cycling in response to photoperiod is observed, and this very low amplitude cycling of some phytochrome proteins is out of phase with previously reported cycling ofPHY mRNA levels. These studies indicate that, with the exception of phytochrome A, the family of phytochrome photoreceptors in Arabidopsis constitutes a quite stable and very broadly distributed array of sensory molecules.

show abstract

“…Thus, these mutants display an elongated growth habit, are defective in end-of-day FR elongation growth responses, flower early compared with their isogenic wild types, and respond poorly to reduced R/FR ratio (10,14,24). Both of these mutants have been shown to be wholly or partially deficient in a phytochrome polypeptide that can be detected with monoclonal antibodies directed against phytochrome B (13,14,22). For hy3, it has also been established that, compared with wild-type seedlings, hybridizable phyB mRNA levels are reduced (22).…”

mentioning

confidence: 99%

Photophysiology of the Elongated Internode (ein) Mutant of Brassica rapa

Devlin

Rood²,

Somers³

et al. 1992

Plant Physiol.

116

View full text Add to dashboard Cite

Several phytochrome-controlled processes have been examined in etiolated and light-grown seedlings of a normal genotype and the elongated internode (ein/ein) mutant of rapid-cycling Brassica rapa. Although etiolated ein seedlings displayed normal sensitivity to prolonged far-red light with respect to inhibition of hypocotyl elongation, expansion of cotyledons, and synthesis of anthocyanin, they displayed reduced sensitivity to prolonged red light for all three of these deetiolation responses. In contrast to normal seedlings, light-grown ein seedlings did not show a growth promotion in response to end-of-day far-red irradiation. Additionally, whereas the first internode of light-grown normal seedlings showed a marked increase in elongation in response to reduced ratio of red to far-red light, ein seedlings showed only a small elongation response. When blots of protein extracts from etiolated and lighttreated ein and normal seedlings were probed with monoclonal antibody to phytochrome A, an immunostaining band at about 120 kD was observed for both extracts. The immunostaining intensity of this band was substantially reduced for extracts of light-treated normal and ein seedlings. A mixture of three monoclonal antibodies directed against phytochrome B from Arabidopsis thaliana immunostained a band at about 120 kD for extracts of etiolated and light-treated normal seedlings. This band was undetectable in extracts of ein seedlings. We propose that ein is a photoreceptor mutant that is deficient in a light-stable phytochrome B-like species.

show abstract

The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome.

Cited by 136 publications

References 33 publications

Phytochrome, Gibberellins, and Hypocotyl Growth (A Study Using the Cucumber (Cucumis sativus L.) long hypocotyl Mutant)

Phytochrome, Gibberellins, and Hypocotyl Growth (A Study Using the Cucumber (Cucumis sativus L.) long hypocotyl Mutant)

Patterns of Expression and Normalized Levels of the Five Arabidopsis Phytochromes

Photophysiology of the Elongated Internode (ein) Mutant of Brassica rapa

Contact Info

Product

Resources

About