The Cultivation of Chelidonium majus L. Increased the Total Alkaloid Content and Cytotoxic Activity Compared with Those of Wild-Grown Plants

Abstract

The effect of cultivation practises on both the phytochemical profile and biological activity of aqueous ethanol extracts of Chelidonium majus L. was studied. Extracts were prepared from aerial parts of the same plant population collected in the wild and grown under organic farming conditions. Both qualitative and quantitative analyses of alkaloids and flavonoid derivatives were performed by LC/MS methods, and the cytotoxicity of lyophilised extracts was studied in B16-F10, HepG2, and CaCo-2 cells. Coptisine was the dominant alkaloid of extracts prepared from wild-grown plants, whereas after cultivation, chelidonine was the most abundant alkaloid. The total alkaloid content was significantly increased by cultivation. Ten flavonol glycoconjugates were identified in C. majus extracts, and quantitative analysis did not reveal significant differences between extracts prepared from wild-grown and cultivated specimens. Treatment with C. majus extracts resulted in a dose-dependent increase in cytotoxicity in all three cell lines. The extracts prepared from cultivated specimens showed higher cytotoxicity than the extracts prepared from wild-grown plants. The strongest cytotoxic effect of cultivated C. majus was observed in B16-F10 cells (IC50 = 174.98 ± 1.12 µg/mL). Cultivation-induced differences in the phytochemical composition of C. majus extracts resulted in significant increases in the cytotoxic activities of the preparations.

Identification of Flavonoids, Antioxidant and Antiproliferative Activity of Aqueous Infusions of Calendula officinalis L., Chelidonium majus L., Teucrium chamaedrys L. and Alchemilla vulgaris L.

“…Quercetin was also found as the predominant flavonoid aglycone in a C. majus extract from Poland, whereas isorhamnetin was not identified at all ( 27 ). Quercetin-3- O -rutinoside as the predominant compound and isorhamnetin-3- O -rutinoside as the second most abundant flavonol glycoside were detected in extracts of wild and cultivated C. majus ( 28 ). Fig.…”

“…The most important flavonoids of C. officinalis are rutin, isorhamnetin and quercetin ( 7 ). Extracts from Chelidonium majus L. ( Papaveraceae ) show antibacterial, antiviral, antifungal and anti-inflammatory effects ( 9 ). The species is important in herbal medicine as a remedy for whooping cough, chronic bronchitis, asthma, gallstones, insomnia and anxiety ( 10 , 11 ).…”

HR LC-MS/MS metabolomic profiling of Yucca aloifolia fruit and the potential neuroprotective effect on rotenone-induced Parkinson’s disease in rats

“…1483 and 611.1608. Fragmentation in the positive ion mode showed the base peak for the quercetin aglycone at m/z 303 and the intermediate peak at 465 due to the loss of the rhamnose moiety [M+H-146] [ 28 ]. Later, the monoglucoside quercetin hexoside was detected in the positive mode at its charged molecular ion at m/z 465.1028 at 6.81 min.…”

A Sustainable Approach to a Cleaner Production of Antimicrobial and Biocompatible Protein Fibers

“…Different parts of the plant are used in various topical applications, including wart removal, treatment of corns, eczema, and cuts, the treatment of skin wounds, skin irritation, allergic rashes, and dermatitis, in the treatment of boils, and cure blisters, rashes, scabies, skin eruptions, psoriasis, eczema, fungal infections and tumors of the skin [ 4 , 7 ].…”