The Curative Effect of Methylsulfonylmethane on an Experimental Model of Ulcerative Colitis in Rats

Abstract

Objective: Ulcerative colitis is a chronic inflammatory bowel disease of unknown etiology that affects the mucosa and submucosa of the colon and rectum. It is associated with oxidative stress, neutrophil infiltration and production of inflammatory mediators. In the present study, the anti-inflammatory and antioxidant effects of Methylsulfonylmethane (MSM) in treatment the acetic acid (A) induced model for UC in rats were examined.
 Methods: Ulcerative colitis was induced by intra colonic administration of 2 ml of 4% acetic acid in adult male Wistar rats which were divided into 3 groups: normal control (N), acetic acid (A) control, and MSM (1000 mg/kg). Rats received treatment for six consecutive days. On day 7, the rats were sacrificed, the colon was removed, the body weight, colon weight/length ratio, tissue glutathione (GSH) concentration, and macroscopic evaluations, were performed.
 Results: The colon weight/length ratio was decreased significantly (????<0.05). The glutathione (GSH) concentration was increased significantly (????<0.05). The macroscopic parameters were decreased, but it didn’t reduce significantly in the MSM treated groups compared to group A. Contrariwise the parameters of group A. The levels of body weight were increased, and colon weight/length ratio was decreased significantly (????<0.05). The glutathione (GSH) concentration was increased significantly (????<0.05). The macroscopic parameters were decreased significantly in the MSM treated group, compared to the acetic acid-treated group.
 Conclusions: Methylsulfonylmethane is an effective, safety natural product with little side effects, has a good curative effect in a dose (1000 mg/kg) on experimental Ulcerative colitis induced by acetic acid.

Putative Role of Chloroquine In Amelioration of DSS Induced

“…The histopathological artifacts observed were scored according to the criteria described previously by Cooper et al in the year of 2011 with slight modification the score represented as bar graph . 17 Normal histological appearance score 0 , Mild changes in texture score 1…”

Recent Approaches Involved in Colonic Drug Targeting for the Treatment of Inflammatory Bowel Disease: A Review

“…Novel site-specific colon targeted approaches Reference Systemic absorption takes place because of delivery to nonspecific cells Systemic absorption is very low due to its site-specific nature [1] Side effects are possible due to systemic absorption of drugs Very minimal side effects due to its site-specific nature [1,4] High doses of the drug have to be administered for obtaining the desired therapeutic effect Reduction of doses is possible. The desired therapeutic effect can be obtained with very low doses [1] Decreased patient compliance due to high frequency and dose of drugs administered Increased patient compliance due to less frequency and dose of drugs administered [4,[7][8][9][10]…”

“…Further to the above approaches, there are novel approaches such as (i) pressure controlled delivery, (ii) CODESTM (combined approach of pH-dependent and microbially triggered delivery), (iii) osmotic drug delivery, and (iv) multiparticulate systems like microspheres and nanoparticles [6]. These approaches are developed for the therapy of IBD for increasing drug localization and accessibility of drug at the target site, reduction of dose, and side-effects associated with the conventional drug delivery systems [4,[7][8][9][10].…”