The current knowledge on the application of anti-biofilm enzymes in the food industry

Meireles, Ana; Borges, Anabela; Giaouris, Efstathios; Simões, Manuel

doi:10.1016/j.foodres.2016.06.006

Cited by 99 publications

(42 citation statements)

References 75 publications

(90 reference statements)

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“…Enzymes have been previously used as a biofilm removal strategy due to their specificity and their low environmental impact (Cordeiro & Werner 2011;Thallinger et al 2013;Meireles et al 2016). In this work, comparison between the effects of cellulase (CEL), DNaseI and pronase (PRN) demonstrated a maximum effect of a 400 µg/ml of DNaseI solution reducing about 2 log CFU/cm 2 the number of AVC in L. monocytogenes and E.…”

Section: 2) Almeida Et Al Reported That In 48 H L Monocytogenesmentioning

confidence: 92%

“…Thus, the idea of a combination of enzymes would be an interesting option to be considered for proper biofilm removal (Simões et al 2010;Meireles et al 2016) especially when dealing with Gram-negatives such as Pseudomonas sp. (Stiefel et al 2016).…”

mentioning

confidence: 99%

“…As a consequence, enzyme based disinfection may need to be performed in combination with biocides that are able to kill the cells avoiding the dispersion of live cells released from the biofilm (Meireles et al 2016;Stiefel et al 2016). …”

mentioning

confidence: 99%

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Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

et al. 2016

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Section: 2) Almeida Et Al Reported That In 48 H L Monocytogenesmentioning

confidence: 92%

mentioning

confidence: 99%

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Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

et al. 2016

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“…The rapid growth in interest in finding potent new applications for use in sanitization programs has stimulated research into novel possibilities regarding enzymatic technology (Khelissa, Abdallah, Jama, Faille, & Chihib, 2017). The employment of enzymatic treatments to break down EPS has become an alternative method because in certain situations the results of using standard cleaning products to remove biofilms are unacceptable (Meireles, Borges, Giaouris, & Simões, 2016). Because the biofilm matrix may be highly heterogenic, a combination of different enzyme activities might be needed for its sufficient degradation.…”

Section: Introductionmentioning

confidence: 99%

Removal of Salmonella enterica serovar Typhimurium and Cronobacter sakazakii biofilms from food contact surfaces through enzymatic catalysis

Ripolles‐Avila

Ríos-Castillo

Fontecha‐Umaña

et al. 2019

Journal of Food Safety

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Bacterial biofilms are highly difficult to control, hence significant economic resources have been allocated to develop strategies to eradicate them. This study evaluated the effect of an enzymatic treatment to be used as a cleaning product to control the presence of biofilms. Two different materials used in the food industry, polystyrene and stainless steel, were tested using Salmonella Typhimuirum and Cronobacter sakazakii. Biofilm formation was carried out by inoculating the surfaces with a standardized concentration of 4 log (CFU cm−2) and incubated for 48 hr with renewal of nutrients. The biofilm formation and subsequent enzymatic treatment were quantified using fluorescence microscopy and the conventional culture method. The enzymatic treatment showed significant reductions of 2–3 log (CFU cm−2) in biofilm cells, which was attributed to the degradation of the extracellular matrix and the further detachment of both microorganisms. The maximum biofilm detachment obtained with the preventive formula was 46.67%; however, this percentage could be increased by applying an aggressive treatment or by adding a subsequent disinfection step that would eliminate adhered microbial cells. Further, the enzymatic cleaning treatment could be exploited as a potent technology to control bacterial adherence and biofilm formation in the food industry.

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“…alcohol, antibiotics, and starvation (9). Genetic elements involved in such responses are mainly rsb and agr genes.…”

Section: Introductionmentioning

confidence: 99%

Overview of Genetic Background Beyond Polysaccharide Intercellular Adhesion Production in Staphylococcus epidermidis

Mekni¹,

Achour²,

Hassen³

2016

Jundishapur J Microbiol

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Background: An important observation during quantification experiments of Staphylococcus epidermidis biofilm is that there is a great difference in the biofilm biomass of different strains despite the same experimental conditions. Objectives: This study aimed to study the genotypic background beyond differential rates of Polysaccharide Intercellular Adhesion (PIA) production in S. epidermidis biofilm forming strains. Methods: A number of 126 strains were isolated from blood cultures (n = 40), catheter cultures (n = 50), and other specimens (n = 36). The strains were obtained from patients hospitalized at the bone marrow transplant center of Tunis. Biofilm micro-plate assay, hemagglutination, and susceptibility to proteinase K methods were used to assess biofilm characteristics in the studied strains. Conventional and real time PCR were used to assess genotypic background of biofilm formation. Results: Using PCR method, we demonstrated that there is a significant difference in ica genes (P < 0.01) and not in adhesion rsb and sar genes distribution between biofilm forming and non-biofilm forming strains. Almost all strains harbored agr type I. None of studied strains harbored IS256 inside ica operon. Ica-independent biofilm formation was detected in 11 strains that were confirmed to have proteinaceous matrix. Using Kernel density estimation, we established that biofilm biomass was higher in ica-dependent than ica-independent biofilm forming population. Using qRT-PCR, we found a significant correlation between biofilm biomass and RNAIII expression level (r 2 = 0.95); but no correlation was found for biofilm biomass neither with icaA nor with ccpA genes.

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The current knowledge on the application of anti-biofilm enzymes in the food industry

Cited by 99 publications

References 75 publications

Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

Removal of Salmonella enterica serovar Typhimurium and Cronobacter sakazakii biofilms from food contact surfaces through enzymatic catalysis

Overview of Genetic Background Beyond Polysaccharide Intercellular Adhesion Production in Staphylococcus epidermidis

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