The current state of the art in gellan-based printing inks in tissue engineering

Cernencu, Alexandra I.; Ioniță, Mariana

doi:10.1016/j.carbpol.2023.120676

Cited by 18 publications

(12 citation statements)

References 50 publications

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“…One of its main drawbacks is the lack of mechanical strength and stability, which can be improved by combining Ge with other polysaccharides to give tough IPN systems 44,45 . Different biopolymer blends have been studied to enhance the processability and functionality of the bioink 46 . Therefore, several Ge‐based IPNs with increased mechanical properties and accuracy were proposed as potential matrices able to support tissue regeneration 47–49 .…”

Section: Resultsmentioning

confidence: 99%

“…44,45 Different biopolymer blends have been studied to enhance the processability and functionality of the bioink. 46 Therefore, several Ge-based IPNs with increased mechanical properties and accuracy were proposed as potential matrices able to support tissue regeneration. [47][48][49] Despite its interesting properties, unmodified Ge is a relatively bioinert material, showing a weak ability to support cell adhesion that limits its applications to anchorageindependent cells.…”

Section: Statistical Analysesmentioning

confidence: 99%

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3D printing of gellan‐dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives

Paoletti,

Baschieri,

Migliorini

et al. 2024

J Biomedical Materials Res

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The ever‐growing need for new tissue and organ replacement approaches paved the way for tissue engineering. Successful tissue regeneration requires an appropriate scaffold, which allows cell adhesion and provides mechanical support during tissue repair. In this light, an interpenetrating polymer network (IPN) system based on biocompatible polysaccharides, dextran (Dex) and gellan (Ge), was designed and proposed as a surface that facilitates cell adhesion in tissue engineering applications. The new matrix was developed in glycerol, an unconventional solvent, before the chemical functionalization of the polymer backbone, which provides the system with enhanced properties, such as increased stiffness and bioadhesiveness. Dex was modified introducing methacrylic groups, which are known to be sensitive to UV light. At the same time, Ge was functionalized with RGD moieties, known as promoters for cell adhesion. The printability of the systems was evaluated by exploiting the ability of glycerol to act as a co‐initiator in the process, speeding up the kinetics of crosslinking. Following semi‐IPNs formation, the solvent was removed by extensive solvent exchange with HEPES and CaCl2, leading to conversion into IPNs due to the ionic gelation of Ge chains. Mechanical properties were investigated and IPNs ability to promote osteoblasts adhesion was evaluated on thin‐layer, 3D‐printed disk films. Our results show a significant increase in adhesion on hydrogels decorated with RGD moieties, where osteoblasts adopted the spindle‐shaped morphology typical of adherent mesenchymal cells. Our findings support the use of RGD‐decorated Ge/Dex IPNs as new matrices able to support and facilitate cell adhesion in the perspective of bone tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Statistical Analysesmentioning

confidence: 99%

3D printing of gellan‐dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives

Paoletti,

Baschieri,

Migliorini

et al. 2024

J Biomedical Materials Res

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“…Besides, it is worth noting that gelatin is mostly used in combination with other materials in bioinks, and its main function is to maintain the shape of the printing scaffold before ink crosslinking. After 2018, more materials have been discovered to be suitable for use as bio-inks., including chitosan ( Kołodziejska et al 2021 ; Xu J. et al 2022 ; Lazaridou et al 2022 ), gellan gum ( Cernencu and Ioniță, 2023 ), hydroxyapatite ( Heid and Boccaccini, 2020 ), pectin ( Mahendiran et al 2021 ; Merli et al 2022 ), cellulose ( Ahlfeld et al 2020 ; Zennifer et al 2021 ) and polysaccharides ( Naranda et al 2021 ; Teixeira et al 2022 ). Meanwhile, we also noticed that dECM, GelMA, and alginate have received more attention recently.…”

Section: Discussionmentioning

confidence: 99%

Global hotspots and emerging trends in 3D bioprinting research

Ding

Tang

Huang

et al. 2023

Front. Bioeng. Biotechnol.

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Three-dimensional (3D) bioprinting is an advanced tissue engineering technique that has received a lot of interest in the past years. We aimed to highlight the characteristics of articles on 3D bioprinting, especially in terms of research hotspots and focus. Publications related to 3D bioprinting from 2007 to 2022 were acquired from the Web of Science Core Collection database. We have used VOSviewer, CiteSpace, and R-bibliometrix to perform various analyses on 3,327 published articles. The number of annual publications is increasing globally, a trend expected to continue. The United States and China were the most productive countries with the closest cooperation and the most research and development investment funds in this field. Harvard Medical School and Tsinghua University are the top-ranked institutions in the United States and China, respectively. Dr. Anthony Atala and Dr. Ali Khademhosseini, the most productive researchers in 3D bioprinting, may provide cooperation opportunities for interested researchers. Tissue Engineering Part A contributed the largest publication number, while Frontiers in Bioengineering and Biotechnology was the most attractive journal with the most potential. As for the keywords in 3D bioprinting, Bio-ink, Hydrogels (especially GelMA and Gelatin), Scaffold (especially decellularized extracellular matrix), extrusion-based bioprinting, tissue engineering, and in vitro models (organoids particularly) are research hotspots analyzed in the current study. Specifically, the research topics “new bio-ink investigation,” “modification of extrusion-based bioprinting for cell viability and vascularization,” “application of 3D bioprinting in organoids and in vitro model” and “research in personalized and regenerative medicine” were predicted to be hotspots for future research.

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“…[1][2][3] The structural similarity of the hydrogels composed of 3D hydrophilic networks to tissues, as well as their high-water contents of 80-99% and low coefficient frictions, make them ideal alternative scaffolds for tissue engineering. 4,5 An ideal hydrogel scaffold should possess good biosafety, strong mechanical support, and customized external geometry. 2,6 With the emphasis on environmentally friendly concepts and the increasing cost of synthetic petroleum-based chemical polymers, sustainable natural polymerbased hydrogels derived from biomass have been developed.…”

Section: Introductionmentioning

confidence: 99%

“…Great efforts have been made to enhance the mechanical support of natural polymer-based hydrogels. 5,13 Chemically and physically crosslinked double network hydrogels are well developed to improve the strength of hydrogels. However, chemical crosslinking methods usually leave unreacted chemical residues and cause poor biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

A natural polymer-based hydrogel with shape controllability and high toughness and its application to efficient osteochondral regeneration

et al. 2023

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The current state of the art in gellan-based printing inks in tissue engineering

Cited by 18 publications

References 50 publications

3D printing of gellan‐dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives

3D printing of gellan‐dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives

Global hotspots and emerging trends in 3D bioprinting research

A natural polymer-based hydrogel with shape controllability and high toughness and its application to efficient osteochondral regeneration

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