The cytogenetic map of the domestic pig (Sus scrofa domestica)

Yerle, Martine M.; Lahbib-Mansais, Y.; Pinton, Philippe; Robic, Annie; Goureau, A.; Milan, Denis; Gellin, Joël

doi:10.1007/s003359900512

Cited by 41 publications

(28 citation statements)

References 189 publications

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“…Genes mapped in this study are displayed in bold face type. Previously reported markers were used for RH map analysis, linkage analysis and map construction (Archibald et al, 1995;Rohrer et al, 1996, Yerle et al, 1997Hawken et al, 1999). RH map position is in centi-Rays, the sex-averaged linkage map position is in centi-Morgans and chromosome ideograms were drawn to represent the standard porcine karyotype described by Gustavsson (1988).…”

Section: Resultsmentioning

confidence: 99%

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Farber

Raney

Rilington

et al. 2003

Cytogenet Genome Res

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Genes located on human chromosome 12 (HSA12) are conserved on pig chromosomes 5 and 14 (SSC5 and SSC14), with HSA12q23.3→q24.11 harboring the evolutionary breakpoint between these chromosomes. For this study, pig sequence-tagged sites (STS) were developed for nine HSA12 genes flanking this breakpoint. Radiation hybrid (RH) mapping using the IMpRH panel revealed that COL2A1, DUSP6, KITLG, PAH and STAB2 map to SSC5, while PXN, PLA2G1B, SART3 and TCF1 map to SSC14. Polymorphisms identified in COL2A1, DUSP6, PAH, PLA2G1B and TCF1 were used for genetic linkage mapping and confirmed the map locations for these genes. Our results indicate that the HSA12 evolutionary breakpoint occurs between STAB2 and SART3 in a region spanning less than five million basepairs. These results refine the comparative map of the HSA12 evolutionary breakpoint region and help to further elucidate the extensive gene order rearrangements between HSA12 and SSC5 and 14.

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Section: Resultsmentioning

confidence: 99%

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Farber

Raney

Rilington

et al. 2003

Cytogenet Genome Res

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“…The total number of assigned markers represents a 10 % increase in physically assigned sequences (Yerle et al, 1997) and a 39 % increase in Type II anchor loci present in the MARC porcine genetic map . Markers were assigned to telomeric bands for 13 chromosomal regions (SSC 1p,3p,4q,5q,9p,9q,10q,12q,13q,15q,18q,Xp,and Xq).…”

Section: Discussionmentioning

confidence: 99%

“…This information is essential to construct a contiguous set of genomic clones and to isolate genes for quantitative trait loci (QTL) defined only by genetic map intervals. Yerle et al (1997) have summarized physical assignments for 436 loci in the pig, including those made by direct in situ single copy-polymerase chain reaction (DISC-PCR; Troyer et al, 1995), somatic cell hybrid, and in situ hybridization for Type I and II loci. This synopsis represents the most extensive physical map in a livestock species.…”

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confidence: 99%

Cytogenetic assignment of 53 microsatellites from the USDA-MARC porcine genetic map

Lopez-Corrales

Beattie

Rohrer³

1999

Cytogenet Genome Res

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This study provides 53 new fluorescent in situ hybridization cytogenetic assignments for microsatellite markers linked on the swine genetic map. Forty microsatellites are physically assigned for the first time. The chromosomal locations of eight markers were either confirmed or refined, while five loci were assigned to locations different from those given in previous reports. Markers were selected to provide physical anchors based on their presumed proximity to centromeres or telomeres and at approximately 30 cM intervals across the genetic map. The number of physical anchors for pig (SSC) chromosomes 8, 15, and 18 linkage groups was significantly improved. Centromeric regions were localized to areas less than 10 cM for SSC 1, 2, 3, 6, 7, 8, and 9. Although the recombination rate was generally higher across small biarmed chromosomes and lowest for large acrocentric chromosomes, two regions with particularly low (1q2.1→q2.9 and 13q2.3→q4.1) and three regions with extremely high (5p1.5→p1.2, 6p1.4→p1.3, and 12p1.5→p1.4) rates of recombination were detected. These assignments represent an overall 10% increase in the number of physically assigned markers in Sus scrofa and more than a 20% increase in the number of Type II loci assigned to the pig cytogenetic map.

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“…We decided to use a representative from a third order of mammals, the domestic pig (Sus scrofa) to enhance investigations into the evolution of this receptor family. The pig gene map is relatively dense (Yerle et al 1997) compared with many other mammals (excluding human or mouse) (O'Brien et al 1999), it is well characterized with respect to the role of NPY in cardiovascular regulation (Malmstrom et al 1998) and is interesting for studying the affects of the NPY system in feeding and metabolism. We report here the isolation of all currently identified NPY receptor genes from the pig, the receptor sequences, and their chromosomal localization within the genome.…”

Section: Af227956 (For Pig Y6 Sequence)]mentioning

confidence: 99%

Evolution of the Neuropeptide Y Receptor Family: Gene and Chromosome Duplications Deduced from the Cloning and Mapping of the Five Receptor Subtype Genes in Pig

Wraith¹,

Törnsten²,

Chardon³

et al. 2000

Genome Res.

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Neuropeptide Y (NPY) receptors mediate a variety of physiological responses including feeding and vasoconstriction. To investigate the evolutionary events that have generated this receptor family, we have sequenced and determined the chromosomal localizations of all five presently known mammalian NPY receptor subtype genes in the domestic pig, Sus scrofa (SSC). The orthologs of the Y 1 and Y 2 subtypes display high amino acid sequence identities between pig, human, and mouse (92%-94%), whereas the Y 4 , Y 5 , and y 6 subtypes display lower identities (76%-87%). The lower identity of Y 5 is due to high sequence divergence in the large third intracellular loop. The NPY1R, NPY2R, and NPY5R receptor genes were localized to SSC8, the NPY4R to SSC14, and NPY6R to SSC2. Our comparisons strongly suggest that the tight cluster of NPY1R, NPY2R, and NPY5R on human chromosome 4 (HSA4) represents the ancestral configuration, whereas the porcine cluster has been split by two inversions on SSC8. These 3 genes, along with adjacent genes from 14 other gene families, form a cluster on HSA4 with extensive similarities to a cluster on HSA5, where NPY6R and >13 other paralogs reside, as well as another large cluster on HSA10 that includes NPY4R. Thus, these gene families have expanded through large-scale duplications. The sequence comparisons show that the NPY receptor triplet NPY1R-NPY2R-NPY5R existed before these large-scale duplications.

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The cytogenetic map of the domestic pig (Sus scrofa domestica)

Cited by 41 publications

References 189 publications

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig

Cytogenetic assignment of 53 microsatellites from the USDA-MARC porcine genetic map

Evolution of the Neuropeptide Y Receptor Family: Gene and Chromosome Duplications Deduced from the Cloning and Mapping of the Five Receptor Subtype Genes in Pig

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