The Cytogenetics of Neurospora

Perkins, David; Barry, Edward G.

doi:10.1016/s0065-2660(08)60246-1

Cited by 199 publications

(139 citation statements)

References 278 publications

Supporting

Mentioning

130

Contrasting

Unclassified

Order By: Relevance

“…The Neurospora genetic map is estimated to span 1000 cM across its seven linkage groups (Perkins and Barry 1976), so we estimated $200 evenly spaced SNPs would be sufficient to establish linkage (,5 cM) with any locus on the genome. Since many pSNPs were close to others and would thus be functionally redundant in the context of recombination analysis, we performed a simple clustering of pSNPs by physical location and then sought to validate a single SNP that would be representative of the cluster within which it resided.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

Lambreghts¹,

Shi²,

Belden³

et al. 2009

Genetics

View full text Add to dashboard Cite

We report the discovery and validation of a set of single nucleotide polymorphisms (SNPs) between the reference Neurospora crassa strain Oak Ridge and the Mauriceville strain (FGSC 2555), of sufficient density to allow fine mapping of most loci. Sequencing of Mauriceville cDNAs and alignment to the completed genomic sequence of the Oak Ridge strain identified 19,087 putative SNPs. Of these, a subset was validated by cleaved amplified polymorphic sequence (CAPS), a simple and robust PCR-based assay that reliably distinguishes between SNP alleles. Experimental confirmation resulted in the development of 250 CAPS markers distributed evenly over the genome. To demonstrate the applicability of this map, we used bulked segregant analysis followed by interval mapping to locate the csp-1 mutation to a narrow region on LGI. Subsequently, we refined mapping resolution to 74 kbp by developing additional markers, resequenced the candidate gene, NCU02713.3, in the mutant background, and phenocopied the mutation by gene replacement in the WT strain. Together, these techniques demonstrate a generally applicable and straightforward approach for the isolation of novel genes from existing mutants. Data on both putative and validated SNPs are deposited in a customized public database at the Broad Institute, which encourages augmentation by community users.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Given estimates of the total genetic length of the N. crassa genome ranging from 500 ( Jin et al 2007) to 1000 (Perkins and Barry 1976) MU, the SNP map can be expected to contain several markers linked to any given genetic locus. This number is close to the saturation level for this cDNA library, for the chosen assay ( Figure 2C).…”

Section: Resultsmentioning

confidence: 99%

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

Lambreghts¹,

Shi²,

Belden³

et al. 2009

Genetics

View full text Add to dashboard Cite

show abstract

“…In most cases multiply-marked tester strains are used to improve efficiency (Perkins, 1990;Perkins, 1991). However, new mutations often fail to show linkage to any of the markers in these strains (Perkins, 2006). When this occurs one must conduct cosegregation analysis using a series of marked strains that collectively test for linkage to regions within each arm of the seven linkage groups.…”

Section: Introductionmentioning

confidence: 99%

Rapid genetic mapping in Neurospora crassa

Jin

Allan

Baber

et al. 2007

Fungal Genetics and Biology

View full text Add to dashboard Cite

Forward genetic analysis is the most broadly applicable approach to discern gene functions. However, for some organisms like the filamentous ascomycete Neurospora crassa, genetic mapping frequently represents a limiting step in forward genetic approaches. We describe an efficient method for genetic mapping in N. crassa that makes use of a modified bulked segregant analysis and PCR-based molecular markers. This method enables mapping with progeny from a single cross, and requires only 90 PCR amplifications. Genetic distances between syntenic markers have been determined to ensure complete coverage of the genome and to allow interpolation of linkage data. As a result, most mutations should be mapped in less than one month to within 1-5 map units, a level of resolution sufficient to initiate map-based cloning efforts. This system also will facilitate analyses of recombination at a genome-wide level, and is applicable to other perfect fungi when suitable markers are available.

show abstract

“…have been identified (Perkins & Barry, 1977). Using auxotrophs, Puhalla & Speith (1983) have suggested that at least four nuclear gene loci control heterokaryon incompatibility in Fusarium moniliforme.…”

Section: W O ' G a R R O And J M C L A R K S O Nmentioning

confidence: 99%

Heterokaryon Incompatibility and Heterozygous Diploid Production in Tomato Wilt Isolates of Verticillium dahliae

O'Garro

Clarkson

1988

Microbiology

View full text Add to dashboard Cite

Auxotrophs derived by UV or N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis from three North American, three European and four Australian tomato wilt isolates of Verticillium dahliae were used to investigate heterokaryon compatibility. Ninety 'crosses' involving race-1 and race-2 isolates were attempted using mixed conidial suspensions and a 'hanging drop' technique. Crosses between North American and Australian isolates failed to produce heterokaryons over a 20 d period but within each of these two groups of isolates (with one exception) the frequency of heterokaryon formation was 100%. In crosses involving the three European isolates the frequency of heterokaryon formation varied from 0 to 100%. Two of the European isolates produced heterokaryons both with North American and with Australian isolates. Prototrophic diploid conidia were recovered from 13 of the 30 crosses that produced heterokaryons. The rate of diploid production was greatest in crosses showing 100% compatibility but rare diploids were recovered from two crosses which had produced no observable heterokaryotic growth on minimal medium. The formation of heterozygous diploids between race-1 and race-2 isolates of V. dahliae indicates the potential for the genetic analysis of pathogenicity through the parasexual cycle.

show abstract

The Cytogenetics of Neurospora

Cited by 199 publications

References 278 publications

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

A High-Density Single Nucleotide Polymorphism Map for Neurospora crassa

Rapid genetic mapping in Neurospora crassa

Heterokaryon Incompatibility and Heterozygous Diploid Production in Tomato Wilt Isolates of Verticillium dahliae

Contact Info

Product

Resources

About