The cytotoxic effects of resin‐based sealers on dental pulp stem cells

Trubiani, Oriana; Caputi, Sergio; Iorio, Donato Di; D’Amario, Maurizio; Paludi, Maya; Giancola, Raffaella; Maio, F. Di Nardo Di; Angelis, Francesco De; D’Arcangelo, Camillo

doi:10.1111/j.1365-2591.2010.01720.x

Cited by 24 publications

(24 citation statements)

References 40 publications

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“…As a group, XP demonstrated the lowest cell sur-vival rates, with cell viability values which were lower thanthe main control representing 100% viability. Other authorshave previously reported the low survival rates of XP Bond,with cell viability values of 32% [24], 30% [25] and even 10% [26]. This has been attributed to the higher levels of UDMA,TEGDMA, HEMA and camphorquinone present in the mixturerelative to other adhesives.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility and bond degradation of poly-acrylic acid coated copper iodide-adhesives

et al. 2017

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Objective. To investigate the effect of poly-acrylic acid (PAA) copper iodide (CuI) adhesives onbond degradation, tensile strength, and biocompatibility. Methods. PAA-CuI particles were incorporated into Optibond XTR, Optibond Solo and XP Bondin 0.1 and 0.5 mg/ml. Clearfil SE Protect, an MDPB-containing adhesive, was used as control.The adhesives were applied to human dentin, polymerized and restored with compositein 2 mm-increments. Resin–tensilebond strength after 24 h, 6 months and 1 year. Hourglass specimens (10 × 2 × 1 mm) wereevaluated for ultimate tensile strength (UTS). Cell metabolic function of human gingivalfibroblast cells exposed to adhesive discs (8 × 1 mm) was assessed with MTT assay. Copperrelease from adhesive discs (5 × 1 mm) was evaluated with UV–vis spectrophotometer afterimmersion in 0.9% NaCl for 1, 3, 5, 7, 10, 14, 21 and 30 days. SEM, EDX and XRF were conductedfor microstructure characterization. Results. XTR and Solo did not show degradation when modified with PAA-CuI regardless ofthe concentration. The UTS for adhesives containing PAA-CuI remained unaltered relative tothe controls. The percent viable cells were reduced for Solo 0.5 mg/ml and XP 0.1 or 0.5 mg/mlPAA-CuI. XP demonstrated the highest ion release. For all groups, the highest release wasobserved at days 1 and 14. Significance. PAA-CuI particles prevented the bond degradation of XTR and Solo after 1 yearwithout an effect on the UTS for any adhesive. Cell viability was affected for some adhesives.A similar pattern of copper release was demonstrated for all adhesives.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility and bond degradation of poly-acrylic acid coated copper iodide-adhesives

et al. 2017

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“…Subsequently, dental pulps were exposed using a cylindrical diamond rotary cutting instrument (Intensiv 314, Ø ISO 014, L.8.0 mm; Intensiv, Grancia, Switzerland) mounted on a high-speed handpiece (Bora L; Bien-Air, Bienne, Switzerland) with water-spray cooling. Pulps were then extracted with a sterile dentinal excavator and cut into small pieces and cultured in MSCM medium (Lonza Verviers Company, Belgium), according to established methods [59]. The PDLSCs and DPSCs were obtained and cultured in MSCM medium (Lonza Verviers Company, Belgium) according to Trubiani et al [59].…”

Section: Methodsmentioning

confidence: 99%

Proteome of Human Stem Cells from Periodontal Ligament and Dental Pulp

et al. 2013

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BackgroundMany adult tissues contain a population of stem cells with the ability to regenerate structures similar to the microenvironments from which they are derived in vivo and represent a promising therapy for the regeneration of complex tissues in the clinical disorder. Human adult stem cells (SCs) including bone marrow stem cells (BMSCs), dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) have been characterized for their high proliferative potential, expression of characteristic SC-associated markers and for the plasticity to differentiate in different lineage in vitro.Methodology/Principal FindingsThe aim of this study is to define the molecular features of stem cells from oral tissue by comparing the proteomic profiles obtained with 2-DE followed by MALDI-TOF/TOF of ex-vivo cultured human PDLSCs, DPSCs and BMSCs. Our results showed qualitative similarities in the proteome profiles among the SCs examined including some significant quantitative differences. To enrich the knowledge of oral SCs proteome we performed an analysis in narrow range pH 4–7 and 6–9, and we found that DPSCs vs PDLSCs express differentially regulated proteins that are potentially related to growth, regulation and genesis of neuronal cells, suggesting that SCs derived from oral tissue source populations may possess the potential ability of neuronal differentiation which is very consistent with their neural crest origin.Conclusion/SignificanceThis study identifies some differentially expressed proteins by using comparative analysis between DPSCs and PDLSCs and BMSCs and suggests that stem cells from oral tissue could have a different cell lineage potency compared to BMSCs.

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“…Adhesion promoting, acidic monomers, containing phosphoric, carboxylic or acrylic portions in the molecules, are considered to be responsible for the antibacterial effect of the primers or adhesive solutions [8]. Moreover, most DBAs and dental composites have extensive cytotoxic effects on human dental pulp mesenchymal stem cells and even on salivary cells [9,10,11]. …”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study

et al. 2017

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Literature presents inconsistent results on the antibacterial activity of dentine bonding systems (DBS). Antibacterial activity of adhesive systems depends on several factors, including composition and acidity. Flow cytometry is a novel detection method to measure multiple characteristics of a single cell: total cell number, structural (size, shape), and functional parameters (viability, cell cycle). The LIVE/DEAD® BacLight™ bacterial viability assay was used to evaluate an antibacterial activity of DBS by assessing physical membrane disruption of bacteria mediated by DBS. Ten commercial DBSs: four total-etching (TE), four self-etching (SE) and two selective enamel etching (SEE) were tested. Both total-etching DBS ExciTE F and OptiBond Solo Plus showed comparatively low antibacterial activity against E. faecalis. The lowest activity of all tested TE systems showed Te-Econom Bond. Among SE DBS, G-ænial Bond (92.24% dead cells) followed by Clearfil S3 Bond Plus (88.02%) and Panavia F 2.0 ED Primer II (86.67%) showed the highest antibacterial activity against E. faecalis, which was comparable to isopropranol (positive control). In the present study, self-etching DBS exhibited higher antimicrobial activity than tested total-etching adhesives against E. faecalis.

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The cytotoxic effects of resin‐based sealers on dental pulp stem cells

Abstract: CMF Bond adhesive allowed human dental pulp stem cells to survive and proliferate. All of the other dentine bonding agents had extensive cytotoxic effects.

Cited by 24 publications

References 40 publications

Biocompatibility and bond degradation of poly-acrylic acid coated copper iodide-adhesives

Biocompatibility and bond degradation of poly-acrylic acid coated copper iodide-adhesives

Proteome of Human Stem Cells from Periodontal Ligament and Dental Pulp

Antibacterial Activity of Commercial Dentine Bonding Systems against E. faecalis–Flow Cytometry Study

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