The cytotoxicity evaluation of Kevlar and silicon carbide by MTT assay

Abstract

The MTT test has been widely used as a rapid and sensitive method for screening anticancer drugs. In this paper, we used this method to assess the cytocompatibility of three materials: Kevlar 29, silicon carbide and polyvinyl chloride (PVC) in both a quantitative and a qualitative manner. The materials were prepared by cleaning in 70% ethanol, autoclaved or gammasterilized. Extracts were prepared at four time periods (1, 2, 3 and 4 weeks) and two temperatures (37°C and 80°C). The extracts were used in the MTT assay and the data were collected and analysed with ONEWAY and DUNCAN procedures using the statistical computer package SPSS x. The MTT staining procedure was also used in direct contact with the materials. The result from the MTT assay demonstrated that Kevlar, SiC and PVC extracted at 37 °C were not cytotoxic while PVC extracted at 80 °C did show some cytotoxicity, especially the material that had been gamma-sterilized. In the direct contact test the Kevlar showed no cytotoxicity. The SiC did show some localized toxicity when the material had been autoclaved, however, SiC subject to prior cleaning with ethanol showed no cytotoxicity. The PVC that had been autoclaved caused a cytotoxic response whereas the material that had been gamma-sterilized or cleaned in ethanol showed good cytocompatibility. This paper demonstrates that the MTT staining procedure is a useful technique to study the cytocompatibility of materials in both a quantitative and a qualitative manner. It is also shown that the cellular response to the materials tested is dependent on the method of preparation.

Endothelial cell growth on silicon modified hydrogenated amorphous carbon thin films

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Antibacterial applications of α-Fe2O3/Co3O4 nanocomposites and study of their structural, optical, magnetic and cytotoxic characteristics

“…The MTT assay is a colorimetric assay which upon cleavage by viable cell's mitochondrial enzyme succinate-dehydrogenase yields a blue colored product formazan. The scanning multiwall spectrophotometer is used to read the amount of formazan crystals produced, which is proportional to the number of viable cells present (Mosmann 1983;Denizot and Lang 1986;Wan et al 1994). The breast cancer cell line MCF7 cells were obtained from the National Center for Cell Sciences, Pune, India.…”

Interpenetrated Chitosan-Poly(Acrylic Acid-Co-Acrylamide) Hydrogels. Synthesis, Characterization and Sustained Protein Release Studies

“…They were placed on a roller mixer at 37˚C and the medium was removed at different time periods (8 h, 1, 4, 7 days) [27] and replaced with other 5 mL of fresh medium. All the extracts were obtained under sterile conditions.…”